Institution
BIA Separations (Slovenia)
Company•Ljubljana, Slovenia•
About: BIA Separations (Slovenia) is a company organization based out in Ljubljana, Slovenia. It is known for research contribution in the topics: Monolithic HPLC column & Monolith. The organization has 84 authors who have published 152 publications receiving 4883 citations.
Papers published on a yearly basis
Papers
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TL;DR: The proposed approach suggested by Yamamoto for transfer of gradient methods between columns of different size can be successfully used for method scale-up on this type of column.
29 citations
TL;DR: Enzyme reactor was found to efficiently eliminate RNA contaminants from DNA samples and was active for several weeks of operation and processed 300 column volumes of sample.
28 citations
TL;DR: Investigating properties of commercial metal-chelate methacrylate monoliths-Convective Interaction Media revealed that the maximal capacity is achieved at protein concentration above 60 microg/ml, and dynamic binding capacity and resolution were found to be flow unaffected.
28 citations
TL;DR: It is demonstrated that citric, isocitric, pyruvic, fumaric, malic, and alpha-ketoglutaric acid can be successfully separated using a CIM monolithic column of suitable user-adjustable length and the dynamic binding capacity is found to be flow-unaffected.
Abstract: The separation of organic acids on the anion-exchange monolithic support, commercially available as Convective Interaction Media (CIM), is presented in this study. It is demonstrated that citric, isocitric, pyruvic, fumaric, malic, and α-ketoglutaric acid can be successfully separated using a CIM monolithic column of suitable user-adjustable length. The effect of the mobile phase composition on the separation is investigated. CIM monolithic columns of adjustable length from 3 to 18 mm are compared regarding the resolution and the back pressure. It is shown that the CIM monolithic column of 12 mm in length enables a good separation of all six organic acids within 3 min and exhibits a linear dependence of back pressure versus flow rate. The resolution and the dynamic binding capacity are found to be flow-unaffected. A filtrated sample of bioprocess supernatant is analyzed without previous pretreatment, which indicates the possibility of online monitoring of small molecules during the bioprocess using CIM monolithic columns.
28 citations
TL;DR: IgGs toward different plasma proteins were immobilized via the hydrazide coupling chemistry to provide oriented immobilization and DBC was found to be flow independent and was increasing with the size of bound protein.
27 citations
Authors
Showing all 84 results
| Name | H-index | Papers | Citations |
|---|---|---|---|
| Aleš Štrancar | 38 | 122 | 3748 |
| Djuro Josic | 36 | 177 | 4580 |
| Aleš Podgornik | 36 | 90 | 3274 |
| Thomas Muster | 33 | 67 | 7766 |
| Miloš Barut | 23 | 45 | 1500 |
| Matjaž Peterka | 17 | 32 | 1222 |
| Petra Kramberger | 14 | 23 | 642 |
| Janez Jančar | 11 | 14 | 425 |
| Jana Vidič | 10 | 19 | 686 |
| Nika Lendero Krajnc | 10 | 19 | 395 |
| Urh Černigoj | 9 | 25 | 267 |
| Rok Košir | 9 | 14 | 456 |
| Peter Brne | 8 | 8 | 330 |
| Marko Banjac | 7 | 10 | 206 |
| Lidija Urbas | 7 | 8 | 241 |