Institution
BIA Separations (Slovenia)
Company•Ljubljana, Slovenia•
About: BIA Separations (Slovenia) is a company organization based out in Ljubljana, Slovenia. It is known for research contribution in the topics: Monolithic HPLC column & Monolith. The organization has 84 authors who have published 152 publications receiving 4883 citations.
Papers published on a yearly basis
Papers
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TL;DR: This work focuses on the development of novel CIM hydrazide (HZ) monoliths for the oriented immobilization of antibodies for convective interaction media use in affinity chromatography.
24 citations
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TL;DR: The present study describes a new methodology to quantify and monitor the purity of supercoiled plasmid DNA by using a monolithic column based on anion-exchange chromatography with UV detection, strengthening the possibility of using this monolithiccolumn associated with a powerful analytical method to control the process development of super coiled plasmsid DNA production and purification for therapeutic applications.
24 citations
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TL;DR: The binding capacity for both BSA and soybean trypsin inhibitor increased with increasing temperature and the effect on the dynamic binding capacity of dimers present in the BSA solution has been evaluated and a closed-loop frontal analysis was used to determine the equilibrium binding capacities.
24 citations
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TL;DR: It is shown that electroextraction gives a higher concentration of extracted pDNA than alkaline lysis, suggesting the use of electroporation as a potentially superior method for extracting pDNA from E. coli.
Abstract: The use of plasmid DNA (pDNA) as a pharmaceutical tool has increased since it represents a safer vector for gene transfer compared to viral vectors. Different pDNA extraction methods have been described; among them is alkaline lysis, currently the most commonly used. Although alkaline lysis represents an established method for isolation of pDNA, some drawbacks are recognized, such as entrapment of pDNA in cell debris, leading to lower pDNA recovery; the time-consuming process; and increase of the volume due to the buffers used, all leading to increased cost of production. We compared the concentration of extracted pDNA when two methods for extracting pDNA from Escherichia coli were used: alkaline lysis and a method based on membrane electroporation, electroextraction. At the same time, we also studied the effect of different pulse protocols on bacterial inactivation. The concentration of pDNA was assayed with anion exchange chromatography. When alkaline lysis was used, two incubations of lysis time (5 and 10 min) were compared in terms of the amount of isolated pDNA. We did not observe any difference in pDNA concentration regardless of incubation time used. In electroextraction, different pulse protocols were used in order to exceed the pDNA concentration obtained by alkaline lysis. We show that electroextraction gives a higher concentration of extracted pDNA than alkaline lysis, suggesting the use of electroporation as a potentially superior method for extracting pDNA from E. coli. In addition, electroextraction represents a quicker alternative to alkaline lysis for extracting pDNA.
24 citations
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TL;DR: In this chapter, an overview of such chromatographic bioreactor systems is given and some immobilization techniques to improve enzyme activity are discussed together with modern Chromatographic supports with improved hydrodynamic characteristics to be used in this context.
Abstract: In the last decade there were many papers published on the study of enzyme catalyzed reactions performed in so-called chromatographic reactors. The attractive feature of such systems is that during the course of the reaction the compounds are already separated, which can drive the reaction beyond the thermodynamic equilibrium as well as remove putative inhibitors. In this chapter, an overview of such chromatographic bioreactor systems is given. Besides, some immobilization techniques to improve enzyme activity are discussed together with modern chromatographic supports with improved hydrodynamic characteristics to be used in this context.
24 citations
Authors
Showing all 84 results
Name | H-index | Papers | Citations |
---|---|---|---|
Aleš Štrancar | 38 | 122 | 3748 |
Djuro Josic | 36 | 177 | 4580 |
Aleš Podgornik | 36 | 90 | 3274 |
Thomas Muster | 33 | 67 | 7766 |
Miloš Barut | 23 | 45 | 1500 |
Matjaž Peterka | 17 | 32 | 1222 |
Petra Kramberger | 14 | 23 | 642 |
Janez Jančar | 11 | 14 | 425 |
Jana Vidič | 10 | 19 | 686 |
Nika Lendero Krajnc | 10 | 19 | 395 |
Urh Černigoj | 9 | 25 | 267 |
Rok Košir | 9 | 14 | 456 |
Peter Brne | 8 | 8 | 330 |
Marko Banjac | 7 | 10 | 206 |
Lidija Urbas | 7 | 8 | 241 |