Institution
Bielefeld University
Education•Bielefeld, Nordrhein-Westfalen, Germany•
About: Bielefeld University is a education organization based out in Bielefeld, Nordrhein-Westfalen, Germany. It is known for research contribution in the topics: Population & Quantum chromodynamics. The organization has 10123 authors who have published 26576 publications receiving 728250 citations. The organization is also known as: University of Bielefeld & UNIVERSITAET BIELEFELD.
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TL;DR: Results show that stromelysin-1, matrilysin, gelatinases A and B and fibroblast and neutrophil collagenases cleave at a common, preferred site in the aggrecan interglobular domain, and additionally that both fibro Blast and neutophil collagenase cleaves at a second sites in the interglOBular domain that is not available to stromelyin or gelatinases.
Abstract: The actions of recombinant human fibroblast collagenase (MMP1), purified polymorphonuclear leucocyte collagenase (MMP8) and their N-terminal catalytic domain fragments against cartilage aggrecan and an aggrecan G1-G2 fragment have been investigated in vitro. After activation with recombinant human stromelysin and typsin, both collagenases were able to degrade human and porcine aggrecans to a similar extent. An N-terminal G1-G2 fragment (150 kDa) was used to identify specific cleavage sites occurring within the proteinase-sensitive interglobular domain between G1 and G2. Two specific sites were found; one at an Asn341-Phe342 bond and another at Asp441-Leu442 (human sequence). This specificity of the collagenases for aggrecan G1-G2 was identical with that of the truncated metalloproteinase matrilysin (MMP7), but different from those of stromelysin (MMP3) and the gelatinases (MMP2 or gelatinase A; MMP9 or gelatinase B) which cleave at the Asn-Phe site, but not the Asp-Leu site. In addition, collagenase catalytic fragments lacking C-terminal hemopexin-like domains were tested and shown to exhibit the same specificities for the G1-G2 fragment as the full-length enzymes. Thus the specificity of the collagenases for cartilage aggrecan was not influenced by the presence or absence of the C-terminal domain. Together with our previous findings, the results show that stromelysin-1, matrilysin, gelatinases A and B and fibroblast and neutrophil collagenases cleave at a common, preferred site in the aggrecan interglobular domain, and additionally that both fibroblast and neutrophil collagenases cleave at a second site in the interglobular domain that is not available to stromelysin or gelatinases.
161 citations
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TL;DR: The lysC/asd gene cluster of Corynebacterium glutamicum ATCC 13032 was cloned and sequenced and found to contain two proteins of Mr 47000 and Mr 18000.
Abstract: The lysC/asd gene cluster of Corynebacterium glutamicum ATCC 13032 was cloned and sequenced. The lysC locus coding for aspartokinase consists of two in-frame overlapping genes, lysC-alpha encoding a protein of 421 amino acids (M(r) 44300) and lysC-beta encoding a protein of 172 amino acids (M(r) 18600). The C. glutamicum aspartokinase was purified and found to contain two proteins of M(r) 47 000 and M(r) 18 000. A C. glutamicum mutant expressing a feedback-resistant aspartokinase was shown to be changed in a single base pair of the lysC-beta gene, leading to an amino acid exchange in the beta-subunit of the aspartokinase. In addition, the identified mutation was found to be responsible for the enhanced expression of the asd gene located downstream of lysC.
161 citations
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TL;DR: The complete DNA sequence of the first mitochondrial genome of a methylotrophic yeast is reported, and the architecture of the putative alpha mating factor protein of P. pastoris CBS7435 turned out to be more complex than the corresponding protein of Saccharomyces cerevisiae.
161 citations
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TL;DR: A significant interaction effect of the endogenous cortisol level with increasing activation in the amygdala under placebo but not under betablocker condition is demonstrated, thereby extending the rodent based model of a synergistic effect ofThe two stress hormones to the human.
161 citations
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01 Jan 2010
160 citations
Authors
Showing all 10375 results
Name | H-index | Papers | Citations |
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Stefan Grimme | 113 | 680 | 105087 |
Alfred Pühler | 102 | 658 | 45871 |
James Barber | 102 | 642 | 42397 |
Swagata Mukherjee | 101 | 1048 | 46234 |
Hans-Joachim Werner | 98 | 317 | 48508 |
Krzysztof Redlich | 98 | 609 | 32693 |
Graham C. Walker | 93 | 381 | 36875 |
Christian Meyer | 93 | 1081 | 38149 |
Muhammad Farooq | 92 | 1341 | 37533 |
Jean Willy Andre Cleymans | 90 | 542 | 27685 |
Bernhard T. Baune | 90 | 608 | 50706 |
Martin Wikelski | 89 | 420 | 25821 |
Niklas Luhmann | 85 | 421 | 42743 |
Achim Müller | 85 | 926 | 35874 |
Oliver T. Wolf | 83 | 337 | 24211 |