scispace - formally typeset
Search or ask a question
Institution

Bielefeld University

EducationBielefeld, Nordrhein-Westfalen, Germany
About: Bielefeld University is a education organization based out in Bielefeld, Nordrhein-Westfalen, Germany. It is known for research contribution in the topics: Population & Quantum chromodynamics. The organization has 10123 authors who have published 26576 publications receiving 728250 citations. The organization is also known as: University of Bielefeld & UNIVERSITAET BIELEFELD.


Papers
More filters
Journal ArticleDOI
TL;DR: Results show that stromelysin-1, matrilysin, gelatinases A and B and fibroblast and neutrophil collagenases cleave at a common, preferred site in the aggrecan interglobular domain, and additionally that both fibro Blast and neutophil collagenase cleaves at a second sites in the interglOBular domain that is not available to stromelyin or gelatinases.
Abstract: The actions of recombinant human fibroblast collagenase (MMP1), purified polymorphonuclear leucocyte collagenase (MMP8) and their N-terminal catalytic domain fragments against cartilage aggrecan and an aggrecan G1-G2 fragment have been investigated in vitro. After activation with recombinant human stromelysin and typsin, both collagenases were able to degrade human and porcine aggrecans to a similar extent. An N-terminal G1-G2 fragment (150 kDa) was used to identify specific cleavage sites occurring within the proteinase-sensitive interglobular domain between G1 and G2. Two specific sites were found; one at an Asn341-Phe342 bond and another at Asp441-Leu442 (human sequence). This specificity of the collagenases for aggrecan G1-G2 was identical with that of the truncated metalloproteinase matrilysin (MMP7), but different from those of stromelysin (MMP3) and the gelatinases (MMP2 or gelatinase A; MMP9 or gelatinase B) which cleave at the Asn-Phe site, but not the Asp-Leu site. In addition, collagenase catalytic fragments lacking C-terminal hemopexin-like domains were tested and shown to exhibit the same specificities for the G1-G2 fragment as the full-length enzymes. Thus the specificity of the collagenases for cartilage aggrecan was not influenced by the presence or absence of the C-terminal domain. Together with our previous findings, the results show that stromelysin-1, matrilysin, gelatinases A and B and fibroblast and neutrophil collagenases cleave at a common, preferred site in the aggrecan interglobular domain, and additionally that both fibroblast and neutrophil collagenases cleave at a second site in the interglobular domain that is not available to stromelysin or gelatinases.

161 citations

Journal ArticleDOI
TL;DR: The lysC/asd gene cluster of Corynebacterium glutamicum ATCC 13032 was cloned and sequenced and found to contain two proteins of Mr 47000 and Mr 18000.
Abstract: The lysC/asd gene cluster of Corynebacterium glutamicum ATCC 13032 was cloned and sequenced. The lysC locus coding for aspartokinase consists of two in-frame overlapping genes, lysC-alpha encoding a protein of 421 amino acids (M(r) 44300) and lysC-beta encoding a protein of 172 amino acids (M(r) 18600). The C. glutamicum aspartokinase was purified and found to contain two proteins of M(r) 47 000 and M(r) 18 000. A C. glutamicum mutant expressing a feedback-resistant aspartokinase was shown to be changed in a single base pair of the lysC-beta gene, leading to an amino acid exchange in the beta-subunit of the aspartokinase. In addition, the identified mutation was found to be responsible for the enhanced expression of the asd gene located downstream of lysC.

161 citations

Journal ArticleDOI
TL;DR: The complete DNA sequence of the first mitochondrial genome of a methylotrophic yeast is reported, and the architecture of the putative alpha mating factor protein of P. pastoris CBS7435 turned out to be more complex than the corresponding protein of Saccharomyces cerevisiae.

161 citations

Journal ArticleDOI
TL;DR: A significant interaction effect of the endogenous cortisol level with increasing activation in the amygdala under placebo but not under betablocker condition is demonstrated, thereby extending the rodent based model of a synergistic effect ofThe two stress hormones to the human.

161 citations


Authors

Showing all 10375 results

NameH-indexPapersCitations
Stefan Grimme113680105087
Alfred Pühler10265845871
James Barber10264242397
Swagata Mukherjee101104846234
Hans-Joachim Werner9831748508
Krzysztof Redlich9860932693
Graham C. Walker9338136875
Christian Meyer93108138149
Muhammad Farooq92134137533
Jean Willy Andre Cleymans9054227685
Bernhard T. Baune9060850706
Martin Wikelski8942025821
Niklas Luhmann8542142743
Achim Müller8592635874
Oliver T. Wolf8333724211
Network Information
Related Institutions (5)
University of Tübingen
84.1K papers, 3M citations

94% related

University of Bonn
86.4K papers, 3.1M citations

94% related

ETH Zurich
122.4K papers, 5.1M citations

93% related

Ludwig Maximilian University of Munich
161.5K papers, 5.7M citations

92% related

Max Planck Society
406.2K papers, 19.5M citations

92% related

Performance
Metrics
No. of papers from the Institution in previous years
YearPapers
2023150
2022511
20211,696
20201,655
20191,410
20181,299