Bowling Green State University

About: Bowling Green State University is a education organization based out in Bowling Green, Ohio, United States. It is known for research contribution in the topics: Population & Poison control. The organization has 8315 authors who have published 16042 publications receiving 482564 citations. The organization is also known as: BGSU.

Actinorhodopsin genes discovered in diverse freshwater habitats and among cultivated freshwater Actinobacteria

Abstract: Microbial rhodopsins are membrane proteins that utilize a retinal chromophore to harvest sunlight for energetic and photosensory functions. Recently, a group of novel rhodopsin sequences named ‘actinorhodopsins’ (ActRs) was hypothesized to exist among uncultured planktonic Actinobacteria. ActRs were discovered by mining metagenomic data obtained during the Venter Institute’s Global Ocean Sampling expedition, from a hypersaline lagoon, two estuaries and a freshwater lake. On the basis of these findings, and many studies that show Actinobacteria are common inhabitants of lakes, we predicted that ActR genes would likely be present in other freshwater habitats and among the genomes of cultivated Actinobacteria. Using degenerate polymerase chain reaction primers, we discovered an ActR gene present in an actinobacterial isolate of the family Microbacteriaceae. Isolate MWH-Uga1 was cultivated prior to this study from a freshwater pond in Uganda and belongs to a group of Actinobacteria previously identified in freshwater ecosystems. ActR genes were also discovered present in numerous mixed cultures containing freshwater Actinobacteria and among environmental DNA samples obtained from three freshwater sources; a small woodland pond and the Laurentian Great Lakes Superior and Erie. An analysis of small subunit ribosomal RNA genes from metagenomic DNA samples harboring ActR genes suggests that organisms belonging to the acI lineage, an uncultured group of Actinobacteria commonly present in fresh waters, may utilize rhodopsins. The co-occurrence of an acI organism with a specific ActR variant in a mixed culture supports our hypothesis.

At the Crossroads of Sexuality and Spirituality: The Sanctification of Sex by College Students.

Abstract: This study examines the intersection between spirituality and premarital sexuality. College students from a Midwestern university (N = 151) completed measures of their beliefs about the sanctification of sexual intercourse in loving, nonmarital relationships. A subsample of 65 participants completed parallel measures regarding current sexual relationships. Greater sanctification was related to increased sexual satisfaction in this subgroup. Increased belief in the sanctification of sexual intercourse was related to increased sexual behavior in the total sample. Finally, the sanctification of sexual intercourse demonstrated incremental validity in the prediction of sexual behavior and satisfaction beyond the effects of attitudes toward premarital sex, dating status, and general religiousness. Overall, these results suggest that sanctification is a unique and useful way to examine the connection between sexuality and spirituality.

Observation and Analysis of Suzuki String Teaching.

Abstract: This study is an examination of teacher and student behavior in 48 violin and cello lessons taught by 12 expert Suzuki string teachers. One representative excerpt of work on a repertoire piece that...

Controlling RNA self-assembly to form filaments.

Abstract: Fundamental control over supra-molecular selfassembly for organization of matter on the nanoscale is a major objective of nanoscience and nanotechnology. ‘RNA tectonics’ is the design of modular RNA units, called tectoRNAs, that can be programmed to self-assemble into novel nano- and mesoscopic architectures of desired size and shape. We report the three-dimensional design of tectoRNAs incorporating modular 4-way junction (4WJ) motifs, hairpin loops and their cognate loop–receptors to create extended, programmable interaction interfaces. Specific and directional RNA–RNA interactions at these interfaces enable conformational, topological and orientational control of tectoRNA selfassembly. The interacting motifs are precisely positioned within the helical arms of the 4WJ to program assembly from only one helical stacking conformation of the 4WJ. TectoRNAs programmed to assemble with orientational compensation produce micrometer-scale RNA filaments through supramolecular equilibrium polymerization. As visualized by transmission electron microscopy, these RNA filaments resemble actin filaments from the protein world. This work emphasizes the potential of RNA as a scaffold for designing and engineering new controllable biomaterials mimicking modern cytoskeletal proteins.

Caught With a Fake ID Ethical Questions About Slippage in Autoethnography

Abstract: Autoethnographers strive to present truthful accounts, the essence of their experience, rather than the Truth or an objective account. There is mindful slippage between Truth and truthful. The author reflects on the emotional consequences of getting “lost” in the slippage of a former partner’s autoethno-graphic writing and contemplates the ethical questions that should be confronted about slippage when making decisions about what to leave in and/or what to leave out. An ethic of accountability for autoethnographic writing is outlined.