Showing papers by "Braunschweig University of Technology published in 2000"

New Syntheses with Oils and Fats as Renewable Raw Materials for the Chemical Industry.

Abstract: Oils and fats are the most important renewable raw materials for the chemical industry. Hitherto, industrial oleochemistry has concentrated predominantly on the carboxy functionality of fatty acids but, more recently, modern synthetic methods have been applied extensively to fatty compounds for the selective functionalization of the alkyl chain. Radical, electrophilic, nucleophilic, and pericyclic as well as transition metal catalyzed additions to the C-C double bond of, for example, oleic acid as the prototype of a readily accessible, unsaturated fatty acid have led to a large number of novel fatty compounds from which interesting properties are expected. Functionalization of C-H bonds in the alkyl chain is also feasible with remarkable selectivity. Effective and highly versatile catalysts for the metathesis of esters of unsaturated fatty acids have been developed, which lead to new and interesting omega-unsaturated fatty acids. The epoxidation of unsaturated fatty acids has been developed extensively. Enzymatic reactions allow syntheses with high selectivity and yield of mono- and diglycerides and esters of carbohydrates with a variety of surfactant properties. Regio- and enantioselective microbial hydrations and hydroxylations widen the spectrum of selective reactions. Of considerable significance is that, with the use of gene technology, natural oils and fats have been improved significantly and will be improved still further, insofar as they show a more uniform and often unusual fatty acid spectrum. Numerous fatty acids are now available in a purity which makes them attractive for synthesis and as raw materials for the chemical industry.

Multiple pathways control protein kinase C phosphorylation.

Abstract: The protein kinase C (PKC) family of signal transducers are characterized by a dependence upon lipids for activity. Specifically, the classical (cPKCα, β and γ) and novel (nPKCδ, ϵ, η and θ) PKC isotypes display a physiological requirement for diacylglycerol for activity. This property of PKC has defined a now well established signalling pathway operating through receptors to phosphatidylinositol‐specific phospholipase C and hence via diacylglycerol (DAG) [and inositol (1,4,5) trisphosphate Ins(1,4,5)P3/Ca2+] to PKC (Figure 1). The operation of this pathway has been described in many cell types, and numerous reviews have covered this signalling paradigm (see Nishizuka, 1986; Hug et al ., 1993; Dekker and Parker, 1994; Jaken, 1996). Figure 1. The classical pathway of PKC activation. The scheme illustrates the production of the immediate precursor lipid PtdIns(4,5)P2 from its parent lipid PtdIns. Various agonists are linked to the phospholipases (PtdIns‐PLC) that can cleave PtdIns(4,5)P2 to diacylglycerol (DAG) and the calcium mobilizer Ins(1,4,5)P3. Calcium can affect the cPKC class by promoting membrane recruitment, but the key allosteric activator at the membrane for both cPKC and nPKC isotypes is DAG. More recently, attention has been drawn to the phosphorylation of PKC itself. Intriguingly, what was once considered a purely effector‐driven transducer turns out to possess a complex amplitude control. The elucidation of this phosphorylation control in the cPKC isotypes has formed the basis for understanding the behaviour of the immediate family, with implications for other related AGC kinases (see Hanks and Hunter, 1995; further information is available at the Protein Kinase Resource: http://www.sdsc.edu/Kinases). The further characterization of the kinases that act upon the nPKCs provides evidence of three distinct input pathways converging upon PKC. Thus, PKC serves as an elegant example of the manner in which multiple signals are integrated …

Embeddings of Gromov hyperbolic spaces

Abstract: It is shown that a Gromov hyperbolic geodesic metric space X with bounded growth at some scale is roughly quasi-isometric to a convex subset of hyperbolic space. If one is allowed to rescale the metric of X by some positive constant, then there is an embedding where distances are distorted by at most an additive constant.

CYK-4: A Rho family gtpase activating protein (GAP) required for central spindle formation and cytokinesis.

Abstract: During cytokinesis of animal cells, the mitotic spindle plays at least two roles. Initially, the spindle positions the contractile ring. Subsequently, the central spindle, which is composed of microtubule bundles that form during anaphase, promotes a late step in cytokinesis. How the central spindle assembles and functions in cytokinesis is poorly understood. The cyk-4 gene has been identified by genetic analysis in Caenorhabditis elegans. Embryos from cyk-4(t1689ts) mutant hermaphrodites initiate, but fail to complete, cytokinesis. These embryos also fail to assemble the central spindle. We show that the cyk-4 gene encodes a GTPase activating protein (GAP) for Rho family GTPases. CYK-4 activates GTP hydrolysis by RhoA, Rac1, and Cdc42 in vitro. RNA-mediated interference of RhoA, Rac1, and Cdc42 indicates that only RhoA is essential for cytokinesis and, thus, RhoA is the likely target of CYK-4 GAP activity for cytokinesis. CYK-4 and a CYK-4:GFP fusion protein localize to the central spindle and persist at cell division remnants. CYK-4 localization is dependent on the kinesin-like protein ZEN-4/CeMKLP1 and vice versa. These data suggest that CYK-4 and ZEN-4/CeMKLP1 cooperate in central spindle assembly. Central spindle localization of CYK-4 could accelerate GTP hydrolysis by RhoA, thereby allowing contractile ring disassembly and completion of cytokinesis.

Saffron - renewed interest in an ancient spice.

Abstract: Research into the chemical composition of saffron, the dried stigmas of Crocus sativus, has seen a renaissance in recent years. Different HPLC protocols for the analysis of saffron constituents have been established, enabling rapid authenticity control of the spice. Saffron flavor has attracted the interest of several research groups. Among the estimated 150 volatile compounds of saffron, approximately 40–50 constituents have so far been identified. Sensory studies allowed the detection of novel key flavor compounds. For some volatiles, generation from acid-labile progenitors was shown. Most recently, a considerable number of non-volatile aroma precursors could be isolated and structurally characterized. This paper reviews the present knowledge about the chemical composition of the world's most expensive spice and gives special emphasis to recent findings on saffron aroma formation.

Extended magnetic reconnection at the Earth's magnetopause from detection of bi-directional jets

Abstract: Magnetic reconnection is a process that converts magnetic energy into bi-directional plasma jets; it is believed to be the dominant process by which solar-wind energy enters the Earth's magnetosphere1,2. This energy is subsequently dissipated by magnetic storms and aurorae3,4. Previous single-spacecraft observations5,6,7 revealed only single jets at the magnetopause—while the existence of a counter-streaming jet was implicitly assumed, no experimental confirmation was available. Here we report in situ two-spacecraft observations of bi-directional jets at the magnetopause, finding evidence for a stable and extended reconnection line; the latter implies substantial entry of the solar wind into the magnetosphere. We conclude that reconnection is determined by large-scale interactions between the solar wind and the magnetosphere, rather than by local conditions at the magnetopause.

3-D shape measurement of complex objects by combining photogrammetry and fringe projection

Abstract: A new concept for 3-D shape measurement of complex ob- jects is proposed, which is based on photogrammetry and fringe projec- tion. The shape of a partial view is determined by a combination of the phase-shift method for fringe evaluation and a photogrammetric triangu- lation to calculate the 3-D coordinates related to the sensor coordinate system. For the measurement of complex objects, partial views are taken from different sensor positions. The problem of matching the par- tial views into each other is solved by transforming each sensor position and the relating point cloud of the shape in a global coordinate system by photogrammetric matching of reference targets. © 2000 Society of Photo- Optical Instrumentation Engineers. (S0091-3286(00)02701-X)

Entanglement of formation for isotropic states

Abstract: We give an explicit expression for the entanglement of formation for isotropic density matrices in arbitrary dimensions in terms of the convex hull of a simple function. For two qutrit isotropic states we determine the convex hull and we have strong evidence for its exact form for arbitrary dimension. Unlike for two qubits, the entanglement of formation for two qutrits or more is found to be a nonanalytic function of the maximally entangled fraction in the regime where the density matrix is entangled.

Plastid Genome Phylogeny and a Model of Amino Acid Substitution for Proteins Encoded by Chloroplast DNA

Abstract: Maximum likelihood (ML) phylogenies based on 9,957 amino acid (AA) sites of 45 proteins encoded in the plastid genomes of Cyanophora, a diatom, a rhodophyte (red algae), a euglenophyte, and five land plants are compared with respect to several properties of the data, including between-site rate variation and aberrant amino acid composition in individual species. Neighbor-joining trees from AA LogDet distances and ML analyses are seen to be congruent when site rate variability was taken into account. Four feasible trees are identified in these analyses, one of which is preferred, and one of which is almost excluded by statistical criteria. A transition probability matrix for the general reversible Markov model of amino acid substitutions is estimated from the data, assuming each of these four trees. In all cases, the tree with diatom and rhodophyte as sister taxa was clearly favored. The new transition matrix based on the best tree, called cpREV, takes into account distinct substitution patterns in plastid-encoded proteins and should be useful in future ML inferences using such data. A second rate matrix, called cpREV*, based on a weighted sum of rate matrices from different trees, is also considered.

Separation and purification of anthocyanins by high-speed countercurrent chromatography and screening for antioxidant activity.

Abstract: The all-liquid chromatographic technique of high-speed countercurrent chromatography (HSCCC) has been applied for separations of anthocyanins. The biphasic mixture of tert-butyl methyl ether/n-butanol/acetonitrile/water (2:2:1:5) acidified with trifluoroacetic acid was found to be a suitable solvent system for anthocyanin separation. In some cases, enrichment of the pigments on Amberlite XAD-7 resin prior to HSCCC has been carried out. The anthocyanin mixtures from red cabbage, black currant, black chokeberry, and roselle were successfully fractionated using HSCCC. Peak purity control was done by nuclear magnetic resonance spectroscopy as well as electrospray ionization ion trap multiple mass spectrometry. Finally, antioxidant activity of the purified pigments was determined using the Trolox equivalent antioxidant capacity test.

Improvement of the mechanical properties of austenitic stainless steel after plasma nitriding

Abstract: In this paper, we report on a series of experiments designed to study the influence of plasma nitriding on the mechanical properties of austenitic stainless steel. Plasma nitriding experiments were conducted on AISI 304L steel in a temperature range of 375–475°C using pulsed-DC plasma with different N2–H2 gas mixtures and treatment times. Firstly the formation and the microstructure of the modified layer will be highlighted followed by the results of hardness measurement, adhesion testing, wear resistance and fatigue life tests. The modified surface was analyzed directly after plasma nitriding as well as using a depth profiling method. The microhardness after plasma nitriding is increased up to 19 GPa, that is a factor of five higher compared to the untreated material (3.3 GPa). The adhesion is examined by Rockwell indentation and scratch test. No delamination of the treated layer could be observed. The wear rate after plasma nitriding is significantly reduced compared to the untreated material. Plasma nitriding produces compressive stress inside the modified layer, which can be easily derived from the bending of thin metal foil, which was treated only on one side. The treatment influences the fatigue life, which can be raised by a factor of 10 at a low stress level (230 MPa).

Suppression of Tumorigenicity in Breast Cancer Cells by the Microfilament Protein Profilin 1

Abstract: Differential display screening was used to reveal differential gene expression between the tumorigenic breast cancer cell line CAL51 and nontumorigenic microcell hybrids obtained after transfer of human chromosome 17 into CAL51. The human profilin 1 (PFN1) gene was found overexpressed in the microcell hybrid clones compared with the parental line, which displayed a low profilin 1 level. A comparison between several different tumorigenic breast cancer cell lines with nontumorigenic lines showed consistently lower profilin 1 levels in the tumor cells. Transfection of PFN1 cDNA into CAL51 cells raised the profilin 1 level, had a prominent effect on cell growth, cytoskeletal organization and spreading, and suppressed tumorigenicity of the stable, PFN1-overexpressing cell clones in nude mice. Immunohistochemical analysis revealed intermediate and low levels of profilin 1 in different human breast cancers. These results suggest profilin 1 as a suppressor of the tumorigenic phenotype of breast cancer cells.

Biosynthesis and Metabolism of Pyrrolizidine Alkaloids in Plants and Specialized Insect Herbivores

Abstract: Pyrrolizidine alkaloids (PAs) encompass a typical class of plant secondary compounds. During recent years PAs have proved to be an excellent choice to exemplify various mechanistic and functional aspects of plant secondary metabolism. PAs appear to play an important role in constitutive plant chemical defense, particularly, in plant-herbivore interactions. Biochemical and physiological aspects of PA biosynthesis, allocation, and accumulation are reviewed with particular emphasis on key enzymatic steps and chemical diversification of PA backbone structures. A number of taxonomically unrelated specialized insect herbivores sequester PAs from their food plants. The biochemistry of PA sequestration in lepidopterans and leaf beetles is outlined along with the mechanisms of PA absorption, storage, pheromone biosynthesis, and insect-specific PA transformations (i.e., formation of insect alkaloids). The unique feature of PAs, that they exist in two easily interchangeable forms, the pro-toxic free base and the nontoxic N-oxide, is stressed and related to the function of PAs as defensive compounds. The first molecular evidence concerning the phylogenetic origin of PAs is presented.

Selenium, the element of the moon, in life on earth.

Abstract: The present status of selenium biochemistry is reviewed with particular emphasis on biomedical problems related to the selenium status of humans and experimental animals. Historical milestones of selenium biochemistry starting from the identification of the first selenoenzymes up to the elucidation of prokaryotic and eukaryotic selenoprotein biosynthesis are compiled. Topical hypotheses on the biological role of selenium in general and of individual selenoproteins in respect to antioxidant defense, redox regulation of metabolic processes, thyroid function, spermatogenesis, oncogenesis, and atherogenesis are critically evaluated.

Physics of mass loaded plasmas

Abstract: In space plasmas the phenomenon of mass loading is common. Comets are one of the most evident objects where mass loading controls to a large extent the structure and dynamics of its plasma environment. New charged material is implanted to the fast streaming solar wind by planets, moons, other solar system objects, and even by the interstellar neutral gas flowing through our solar system. In this review we summarize both the current observations and the relevant theoretical approaches. First we survey the MHD methods, starting with a discussion how mass loading affects subsonic and supersonic gasdynamics flows, continuing this with single and multi-fluid MHD approaches to describe the flow when mass, momentum and energy is added, and we finish this section by the description of mass loaded shocks. Next we consider the kinetic approach to the same problem, discussing wave excitations, pitch angle and energy scattering in linear and quasi-linear approximations. The different descriptions differ in assumptions and conclusions; we point out the differences, but it is beyond the scope of the paper to resolve all the conflicts. Applications of these techniques to comets, planets, artificial ion releases, and to the interplanetary neutrals are reviewed in the last section, where observations are also compared with models, including hybrid simulations as well. We conclude the paper with a summary of the most important open, yet unsolved questions.

Ascosalipyrrolidinone A, an antimicrobial alkaloid, from the obligate marine fungus Ascochyta salicorniae.

Abstract: From the green alga Ulva sp., the endophytic and obligate marine fungus Ascochyta salicorniae was isolated. A. salicorniae was mass cultivated and found to produce the unprecedented and structurally unusual tetramic acid containing metabolites ascosalipyrrolidinones A (1) and B (2). Additionally, the new natural product ascosalipyrone (3) and the known metabolites 4 and 5 were obtained. Ascosalipyrrolidinone A (1) has antiplasmodial activity toward Plasmodium falciparum strains K1 and NF 54, as well as showing antimicrobial activity and inhibiting tyrosine kinase p56lck.

Timing precision in continuation and synchronization tapping.

Abstract: Wing and Kristofferson (1973) have shown that temporal precision in self-paced tapping is limited by variability in a central timekeeper and by variability arising in the peripheral motor system. Here we test an extension of the Wing–Kristofferson model to synchronization with periodic external events that was proposed by Vorberg and Wing (1994). In addition to the timekeeper and motor components, a linear phase correction mechanism is assumed which is triggered by the last or the last two synchronization errors. The model is tested in an experiment that contrasts synchronized and self-paced trapping, with response periods ranging from 200–640 ms. The variances of timekeeper and motor delays and the error correction parameters were estimated from the auto-covariance functions of the inter-response intervals in continuation and the asynchronies in synchronization. Plausible estimates for all parameters were obtained when equal motor variance was assumed for synchronization and continuation. Timekeeper variance increased with metronome period, but more steeply during continuation than during synchronization, suggesting that internal timekeeping processes are stabilized by periodic external signals. First-order error correction became more important as the metronome period increased, whereas the contribution of second-order error correction decreased. It is concluded that the extended two-level model accounts well for both synchronization and continuation performance.

Transient wave propagation in a one-dimensional poroelastic column

Abstract: Biot's theory of porous media governs the wave propagation in a porous, elastic solid infiltrated with fluid. In this theory, a second compressional wave, known as the slow wave, has been identified. In this paper, Biot's theory is applied to a one-dimensional continuum. Despite the simplicity of the geometry, an exact solution of the full model, and a detailed analysis of the phenomenon, so far have not been achieved. In the present approach, an analytical solution in the Laplace transform domain is obtained showing clearly two compressional waves. For the special case of an inviscid fluid, a closed form exact solution in time domain is obtained using an analytical inverse Laplace transform. For the general case of a viscous fluid, solution in time domain is evaluated using the Convolution Quadrature Method of Lubich. Of all the inverse methods previously investigated, it seems that only the method of Lubich is efficies and stable enough to handle the highly transient cases such as impact and step loadings. Using properties of three widely different real materials, the wave propagating behavior, in terms of stress, pore pressure, displacement, and flux, are examined. Of most interest is the identification of second compressional wave and its sensitivity of material parameters.

Meta-heuristics: The State of the Art

Abstract: Meta-heuristics support managers in decision-making with robust tools that provide high-quality solutions to important applications in business, engineering, economics and science in reasonable time horizons. In this paper we give some insight into the state of the art of meta-heuristics. This primarily focuses on the significant progress which general frames within the meta-heuristics field have implied for solving combinatorial optimization problems, mainly those for planning and scheduling.

Monitoring the thermal deformation of steel beams via vision metrology

Abstract: The multi-epoch deformation monitoring of a series of super-hot steel beams by digital close-range photogrammetry is reported An on-line configuration of three CCD cameras was established to measure both stable reference points and targets subject to positional displacement Measurements for each beam were conducted at 70–80 epochs over 2 h as the steel cooled from 1100°C to near room temperature Special targeting was required to accommodate the changing colour of the beams from white-hot to brown as they cooled and ensure target survival through a large temperature range A computational approach was employed whereby the photogrammetric triangulation process for any given recording epoch utilised all images obtained up until that time The paper discusses all aspects of the project in which seven beams were monitored to a dimensional tolerance of close to 1 mm (RMS 1-sigma)