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Institution

Brno University of Technology

EducationBrno, Czechia
About: Brno University of Technology is a education organization based out in Brno, Czechia. It is known for research contribution in the topics: Fracture mechanics & Filter (video). The organization has 6339 authors who have published 15226 publications receiving 194088 citations. The organization is also known as: Vysoké učení technické v Brně & BUT.


Papers
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Journal ArticleDOI
TL;DR: Results show that at much lower concentrations, quercetin (QC), a dietary flavonoid isolated from an edible lichen, can be implemented as a sensitizing agent for FCZ-resistant C. albicans NBC099, enhancing the efficacy of FCZ.
Abstract: Quorum sensing (QS) regulates group behaviors of Candida albicans such as biofilm, hyphal growth, and virulence factors. The sesquiterpene alcohol farnesol, a QS molecule produced by C. albicans, is known to regulate the expression of virulence weapons of this fungus. Fluconazole (FCZ) is a broad-spectrum antifungal drug that is used for the treatment of C. albicans infections. While FCZ can be cytotoxic at high concentrations, our results show that at much lower concentrations, quercetin (QC), a dietary flavonoid isolated from an edible lichen (Usnea longissima), can be implemented as a sensitizing agent for FCZ-resistant C. albicans NBC099, enhancing the efficacy of FCZ. QC enhanced FCZ-mediated cell killing of NBC099 and also induced cell death. These experiments indicated that the combined application of both drugs was FCZ dose dependent rather than QC dose dependent. In addition, we found that QC strongly suppressed the production of virulence weapons—biofilm formation, hyphal development, phospholipase, proteinase, esterase, and hemolytic activity. Treatment with QC also increased FCZ-mediated cell death in NBC099 biofilms. Interestingly, we also found that QC enhances the anticandidal activity of FCZ by inducing apoptotic cell death. We have also established that this sensitization is reliant on the farnesol response generated by QC. Molecular docking studies also support this conclusion and suggest that QC can form hydrogen bonds with Gln969, Thr1105, Ser1108, Arg1109, Asn1110, and Gly1061 in the ATP binding pocket of adenylate cyclase. Thus, this QS-mediated combined sensitizer (QC)-anticandidal agent (FCZ) strategy may be a novel way to enhance the efficacy of FCZ-based therapy of C. albicans infections.

55 citations

Journal ArticleDOI
TL;DR: John T Sheridan, Raymond K Kostuk2, Antonio Fimia Gil, Y Wang4, W Lu4, H Zhong4, Y Tomita5, C Neipp6, J Francés6, S Gallego6, I Pascual6, V Marinova7,8, S-H Lin7, K-Y Hsu7, F Bruder9, S Hansen9, C Manecke9, R Meisenheimer
Abstract: This work was supported by Ministerio de Economia, Industria y Competitividad (Spain) under projects FIS2017-82919-R (MINECO/AEI/FEDER, UE) and FIS2015-66570-P (MINECO/FEDER), and by Generalitat Valenciana (Spain) under project PROMETEO II/2015/015.

55 citations

Journal ArticleDOI
TL;DR: It was found that WS/KL is highly compatible with hyaluronan due to its improved water solubility, which favours its use in designing new advanced composite hydrogels, and the cytotoxicity tests proved that glycinated KL lignin limits the viability of cells only slightly.

55 citations

Journal ArticleDOI
TL;DR: In this paper, electron-donating or withdrawing groups in the p-position of the phenyl were synthesized and studied using optical characterization (absorption, fluorescence, time-resolved fluorescence) and quantum chemical calculation.

55 citations

Journal ArticleDOI
TL;DR: In this paper, the impact of applied chemical agents for surface treatment onto the wettability is analyzed for polyimide, polyethylene terephthalate and polyethylenes naphthalene substrates.
Abstract: Purpose – This paper aims to find an optimal surface treatment of commonly used polymeric substrates for achieve the high adhesion of printed structures. For this reason, the investigation of substrates surfaces from different perspectives is presented in this paper. Design/methodology/approach – The contact angle measurements as well as the roughness measurements were realised for the analysis of surface properties of investigated substrates. The impact of applied chemical agents for surface treatment onto the wettability is analysed for polyimide, polyethylene terephthalate and polyethylene naphthalene substrates. Findings – The results prove the correlation among wettability, surface energy and work of adhesion with respect to the theoretical background. The surface treatment of polymeric substrates by chemical agents, such as acetone, toluene, ethanol, isopropyl and fluor silane polymer, has a significant impact onto the wettability of substrates which affects the final deposition process of nanoinks....

55 citations


Authors

Showing all 6383 results

NameH-indexPapersCitations
Georg Kresse111430244729
Patrik Schmuki10976352669
Michael Schmid8871530874
Robert M. Malina8869138277
Jiří Jaromír Klemeš6456514892
Alessandro Piccolo6228414332
René Kizek6167216554
George Danezis5920911516
Stevo Stević583749832
Edvin Lundgren5728610158
Franz Halberg5575015400
Vojtech Adam5561114442
Lukas Burget5325221375
Jan Cermak532389563
Hynek Hermansky5131714372
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Performance
Metrics
No. of papers from the Institution in previous years
YearPapers
202328
2022106
20211,053
20201,010
20191,214
20181,131