Showing papers by "Central Tuber Crops Research Institute published in 1984"
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TL;DR: A new method is reported for determination of cyanoglucosides in cassava, simple, rapid, and sensitive, and complete CNG extraction and rapid inactivation of endogenous linamarase is possible with 80% ethanol.
Abstract: A new method is reported for determination of cyanoglucosides in cassava. The method is simple, rapid, and sensitive. Ten g cassava tuber is homogenized with warm (65-70 degrees C) 80% ethanol (1 + 6, w/v) to extract cyanoglucosides (CNG). The ethanol is evaporated, and an aliquot of the extract (0.1-0.2 mL) is incubated with added linamarase in pH 6.0 phosphate buffer for 15 min at 30 degrees C. The reaction is stopped by adding 0.2 N sodium hydroxide, the solution is neutralized, and cyanide is estimated by adding chloramine T and barbituric acid-pyridine reagent and measuring the absorbance at 570 nm. Complete CNG extraction and rapid inactivation of endogenous linamarase is possible with 80% ethanol. There is no interference from extractives in the linamarase reaction or in the estimation of cyanide. Recovery of added linamarin (as cyanide) is 98% by this assay. The minimum detection limit of cyanide in the assay is 0.1 micrograms/mL.
21 citations
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TL;DR: The presence of pollen sterility in all the plants of Ce-258 throughout the season ruled out environmental factors and suggested it was of genic nature.
Abstract: A completely male sterile clone was identified in the germplasm and detailed meiosis showed it to be asynaptic. At pachytene all the chromosomes remained as univalents without any semblance to pairing. MI aslo showed 36 univalents in 97% of pollen mother cells. The bivalent like association in MI was as low as 0.03 per cell but the absence of pairing at pachytene and the lack of well defined x'mata suggested that they were only univalents lying closely together due to factors other than true pairing. The later stages were not well defined and univalents were noticed to divide. The scattering of chromosomes and irregular groupings resulted in polyads consequently all the pollen were sterile. The type of asynapsis noticed was ‘complete’. The presence of pollen sterility in all the plants of Ce-258 throughout the season ruled out environmental factors and suggested it was of genic nature.