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Institution

Central Tuber Crops Research Institute

FacilityThiruvananthapuram, Kerala, India
About: Central Tuber Crops Research Institute is a facility organization based out in Thiruvananthapuram, Kerala, India. It is known for research contribution in the topics: Starch & Fermentation. The organization has 475 authors who have published 587 publications receiving 10285 citations.


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TL;DR: MS medium with benzyl adenine at 3 μM and Kinetin at 4 and 5 μM were found to be the best concentrations for developing shoots with good number of nodes and leaves and the addition of 1- naphthaleneacetic acid (NAA) along with cytokinin resulted in healthier leaf production.
Abstract: A protocol was developed for the in vitro propagation and microtuber induction in Dioscorea belophylla using nodal explants at Central Tuber Crops Research Institute, Thiruvananthapuram, India. MS medium with benzyl adenine (BA) at 3 μM and Kinetin (Kin) at 4 and 5 μM were found to be the best concentrations for developing shoots with good number of nodes and leaves. The addition of 1- naphthaleneacetic acid (NAA) along with cytokinin resulted in healthier leaf production. Among the different concentrations of sucrose tested, MS medium with 4% sucrose was the best for shooting, having the highest shoot length of 11.8 cm and mean number of 17.4 nodes and 11.4 leaves/shoot within 15.4 days of inoculation. Microtuber induction was recorded in the presence of BA (3-5 μM), Kin (3-4 μM) and BA 3 μM along with NAA (1 μM). Addition of 3-8% sucrose in the medium was found to be the best concentration for inducing 1-3 microtubers/culture.

2 citations

Journal ArticleDOI
TL;DR: In this paper, the authors investigated the uptake pattern and partitioning of nutrients in the total biomass of healthy and root (wilt) diseased palms grown in humid tropical Entisol of Kerala.
Abstract: Nutrient uptake in the biomass of healthy and root (wilt) disease affected coconut palms are different. Root (wilt) is a predominant phytoplasmal disease affecting coconut (Cocos nucifera L.) palms and to sustain the palm health and productivity, better nutrient management practices are required. The objective of this research was to understand the uptake pattern and the partitioning of nutrients in the total biomass of healthy and root (wilt) diseased palms grown in humid tropical Entisol of Kerala. 5 coconut palms each were selected from diseased palms viz., Early (DE), Middle (DM) and Advanced (DA) category along with the control, which is Apparently Healthy palm (AH), without any diseases. The total biomass component with respect to each part viz., leaf (L), petiole (P), spadix (S), spathe (Sp), stem (St), leaf sheath fiber (LSF), husk (H) and nuts (copra:C + shell (Sh)) (N) were estimated. Nutrient uptake by the entire biomass uptake is classified as 1. Recyclable portion (L, P, S, Sp, H, LSF and Sh) 2. Removable portion (C) 3. Reserve biomass (St) and then computed the nutrient partitioning by standard analytical procedures. For all nutrients except Mg, reserve biomass had the greatest nutrient uptake followed by recyclable biomass and removable biomass components. Among the nutrients, potassium recorded the highest total uptake. In AH palm, the total uptake of potassium was 1075 g per palm, whereas it was 407.3 g per palm in the diseased palms. The total uptake of nutrients which showed a significant relation with disease index followed the order K > N > Ca > S > P > Mg in apparently healthy palms and N > K > Ca > S > P > Mg in diseased palms. The percentage reduction in total uptake of N, P, K, Ca, Mg, S and B by diseased palms in comparison with healthy palms was 36.5, 37.6, 57.5, 44.1 23.3, 43.7 and 48.9. The results of the study indicated that the pattern of nutrient uptake in the different biomass components of apparently healthy and root (wilt) disease affected palms are significantly different. This can instigate the formulation of appropriate nutrient management strategies in coconut with emphasis on its residue recycling potential.

2 citations

Journal ArticleDOI
TL;DR: In this paper, the authors formulated a novel Levilactobacillus brevis enriched nutraceutical and studied its functional property in vitro in cancer cell lines and in vivo in Salmonella enterica serovar Typhimurium infected mouse model.
Abstract: The objective of the study was to formulate a novel Levilactobacillus brevis enriched nutraceutical and to study its functional property in vitro in cancer cell lines and in vivo in Salmonella enterica serovar Typhimurium infected mouse model. The formulation was prepared through the fermentation of carrot and beetroot extracts using L. brevis MTCC 4460 and optimized by response surface methodology and artificial neural networking. The optimized formulation (3.23 mg/ml lactic acid) could be obtained through 48 h fermentation with 2% of bacterial inoculum, 0.67% additional sugar and 30.11% of beetroot extract. The L. brevis MTCC 4460 content in the optimized product was 4 × 109 CFU/ml. GC-MS study indicated the generation of some novel flavouring and bioactive compounds such as γ-decalactone, and 1,2:5,6-dianhydrogalactitol during the fermentation. In vitro study with HCT116 and MDA-MB-231 cancer cell lines elucidated better antiproliferative and antimigratory effect of the optimized formulation. In vivo studies showed that the L. brevis MTCC 4460 could colonize in the colon of the mouse fed with the optimized product. In addition, the formulation effectively prevented Salmonella-induced colitis in the mouse model. Based on the aforesaid findings, the optimized formulation can be recommended as a potential dietary supplement for a healthy lifestyle.

2 citations

Journal ArticleDOI
TL;DR: The study indicates that the use of BAP could be an important approach in controlling tuber rot pathogen, P. palmivora.
Abstract: Auxins and cytokinins are implicated in a wide variety of developmental and physiological processes in plants. Phytophthora palmivora causes tuber rot in cassava growing regions of Tamil Nadu and Kerala, South India. The in vitro effect of cytokinin, benzyl amino purine (BAP) and auxins, naphthalene acetic acid (NAA) and indole acetic acid (IAA) on P. palmivora mycelium growth was investigated. The inhibitory activity varied among the growth regulators and complete inhibition of the pathogen was observed at 50, 2000 and 2500 ppm by the BAP, IAA and NAA, respectively. The effective growth regulator, BAP was also analysed on tubers before and after the invasion of the pathogen to observe its effect in tuber. Further, it was also checked against the bio-control agent Trichoderma harzianum. The study indicates that the use of BAP could be an important approach in controlling tuber rot pathogen, P. palmivora.

2 citations


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Performance
Metrics
No. of papers from the Institution in previous years
YearPapers
20231
20225
202129
202032
201927
201823