Showing papers by "Centre national de la recherche scientifique published in 1996"
[...]
Université catholique de Louvain1, McGill University2, Stanford University3, Pierre-and-Marie-Curie University4, Ludwig Maximilian University of Munich5, Centre national de la recherche scientifique6, École Normale Supérieure7, Washington University in St. Louis8, John Radcliffe Hospital9, Max Planck Society10, University of Basel11, University of Manchester12
TL;DR: The genome of the yeast Saccharomyces cerevisiae has been completely sequenced through a worldwide collaboration and provides information about the higher order organization of yeast's 16 chromosomes and allows some insight into their evolutionary history.
Abstract: The genome of the yeast Saccharomyces cerevisiae has been completely sequenced through a worldwide collaboration. The sequence of 12,068 kilobases defines 5885 potential protein-encoding genes, approximately 140 genes specifying ribosomal RNA, 40 genes for small nuclear RNA molecules, and 275 transfer RNA genes. In addition, the complete sequence provides information about the higher order organization of yeast's 16 chromosomes and allows some insight into their evolutionary history. The genome shows a considerable amount of apparent genetic redundancy, and one of the major problems to be tackled during the next stage of the yeast genome project is to elucidate the biological functions of all of these genes.
4,254 citations
TL;DR: In this article, two statistical tests for detecting a heterozygosity excess are described, and the most useful markers for bottleneck detection are those evolving under the infinite allele model (IAM) and they provide guidelines for selecting sample sizes of individuals and loci.
Abstract: When a population experiences a reduction of its effective size, it generally develops a heterozygosity excess at selectively neutral loci, i.e., the heterozygosity computed from a sample of genes is larger than the heterozygosity expected from the number of alleles found in the sample if the population were at mutation drift equilibrium. The heterozygosity excess persists only a certain number of generations until a new equilibrium is established. Two statistical tests for detecting a heterozygosity excess are described. They require measurements of the number of alleles and heterozygosity at each of several loci from a population sample. The first test determines if the proportion of loci with heterozygosity excess is significantly larger than expected at equilibrium. The second test establishes if the average of standardized differences between observed and expected heterozygosities is significantly different from zero. Type I and II errors have been evaluated by computer simulations, varying sample size, number of loci, bottleneck size, time elapsed since the beginning of the bottleneck and level of variability of loci. These analyses show that the most useful markers for bottleneck detection are those evolving under the infinite allele model (IAM) and they provide guidelines for selecting sample sizes of individuals and loci. The usefulness of these tests for conservation biology is discussed.
4,106 citations
TL;DR: It is shown that mutations in the Toll signaling pathway dramatically reduce survival after fungal infection and the intracellular components of the dorsoventral signaling pathway and the extracellular Toll ligand, spätzle, control expression of the antifungal peptide gene drosomycin in adults.
3,564 citations
TL;DR: The last version of the Généthon human linkage map is reported, which consists of 5,264 short tandem repeat polymorphisms with a mean heterozygosity of 70%.
Abstract: The great increase in successful linkage studies in a number of higher eukaryotes during recent years has essentially resulted from major improvements in reference genetic linkage maps, which at present consist of short tandem repeat polymorphisms of simple sequences or microsatellites. We report here the last version of the Genethon human linkage map. This map consists of 5,264 short tandem (AC/TG)n repeat polymorphisms with a mean heterozygosity of 70%. The map spans a sex-averaged genetic distance of 3,699 cM and comprises 2,335 positions, of which 2,032 could be ordered with an odds ratio of at least 1,000:1 against alternative orders. The average interval size is 1.6 cM; 59% of the map is covered by intervals of 2 cM at most and 1% remains in intervals above 10 cM.
2,982 citations
TL;DR: This paper unify the formulation of these different approaches using transformation theory and an eigenvector analysis of the covariance or coherency matrix of the scattering matrix for target decomposition theory in radar polarimetry.
Abstract: In this paper, we provide a review of the different approaches used for target decomposition theory in radar polarimetry. We classify three main types of theorem; those based on the Mueller matrix and Stokes vector, those using an eigenvector analysis of the covariance or coherency matrix, and those employing coherent decomposition of the scattering matrix. We unify the formulation of these different approaches using transformation theory and an eigenvector analysis. We show how special forms of these decompositions apply for the important case of backscatter from terrain with generic symmetries.
2,369 citations
TL;DR: A Macintosh computer program that can perform many time-series analysis procedures is now available on the Internet free of charge, originally designed for paleoclimatic time series.
Abstract: A Macintosh computer program that can perform many time-series analysis procedures is now available on the Internet free of charge. Although AnalySeries was originally designed for paleoclimatic time series, it can be useful for most fields of Earth sciences. The program's graphical user interface allows easy access even for people unfamiliar with computer calculations. Previous versions of the program are already used by hundreds of scientists worldwide.
2,015 citations
TL;DR: The relaxation processes of electrons and spins systems following the absorption of femtosecondoptical pulses in ferromagnetic nickel have been studied using optical and magneto-optical pump-probetechniques and the experimental results are adequately described by a model including three interacting reservoirs.
Abstract: The relaxation processes of electrons and spins systems following the absorption of femtosecond optical pulses in ferromagnetic nickel have been studied using optical and magneto-optical pump-probe techniques. The magnetization of the film drops rapidly during the first picosecond, but different electron and spin dynamics are observed for delays in the range 0--5 ps. The experimental results are adequately described by a model including three interacting reservoirs (electron, spin, and lattice).
1,920 citations
TL;DR: Investigation of the behavioural effects of morphine reveals that a lack of μ receptors abolishes the analgesic effect of morphine, as well as place-preference activity and physical dependence, and concludes that the µ-opioid-receptor gene product is a mandatory component of the opioid system for morphine action.
Abstract: Despite tremendous efforts in the search for safe, efficacious and non-addictive opioids for pain treatment, morphine remains the most valuable painkiller in contemporary medicine. Opioids exert their pharmacological actions through three opioid-receptor classes, mu, delta and kappa, whose genes have been cloned. Genetic approaches are now available to delineate the contribution of each receptor in opioid function in vivo. Here we disrupt the mu-opioid-receptor gene in mice by homologous recombination and find that there are no overt behavioural abnormalities or major compensatory changes within the opioid system in these animals. Investigation of the behavioural effects of morphine reveals that a lack of mu receptors abolishes the analgesic effect of morphine, as well as place-preference activity and physical dependence. We observed no behavioural responses related to delta- or kappa-receptor activation with morphine, although these receptors are present and bind opioid ligands. We conclude that the mu-opioid-receptor gene product is the molecular target of morphine in vivo and that it is a mandatory component of the opioid system for morphine action.
1,641 citations
TL;DR: It is shown that KVLQT1 associates with IsK to form the channel underlying the IKS cardiac current, which is a target of class-Ill anti-arrhythmic drugs and is involved in the L QT1 syndrome.
Abstract: In mammalian cardiac cells, a variety of transient or sustained K+ currents contribute to the repolarization of action potentials. There are two main components of the delayed-rectifier sustained K+ current, I(Kr) (rapid) and I(Ks), (slow). I(Kr) is the product of the gene HERG, which is altered in the long-QT syndrome, LQT2. A channel with properties similar to those of the I(Ks) channel is produced when the cardiac protein IsK is expressed in Xenopus oocytes. However, it is a small protein with a very unusual structure for a cation channel. The LQT1 gene is another gene associated with the LQT syndrome, a disorder that causes sudden death from ventricular arrhythmias. Here we report the cloning of the full-length mouse K(V)LQT1 complementary DNA and show that K(V)LQT1 associates with IsK to form the channel underlying the I(Ks) cardiac current, which is a target of class-III anti-arrhythmic drugs and is involved in the LQT1 syndrome.
1,603 citations
TL;DR: In this article, the results of low-temperature experiments on a single crystal composed of superparamagnetic manganese clusters (Mn12-ac) were reported, which clearly demonstrate the existence of quantum-mechanical tunnelling of the bulk magnetization.
Abstract: THE precise manner in which quantum-mechanical behaviour at the microscopic level underlies classical behaviour at the macroscopic level remains unclear, despite seventy years of theoretical investigation. Experimentally, the crossover between these regimes can be explored by looking for signatures of quantum-mechanical behaviour—such as tunneling—in macroscopic systems1. Magnetic systems (such as small grains, spin glasses and thin films) are often investigated in this way2–12 because transitions between different magnetic states can be closely monitored. But transitions between states can be induced by thermal fluctuations, as well as by tunnelling, and definitive identification of macroscopic tunnelling events in these complex systems is therefore difficult13. Here we report the results of low-temperature experiments on a single crystal composed of super-paramagnetic manganese clusters (Mn12-ac), which clearly demonstrate the existence of quantum-mechanical tunnelling of the bulk magnetization. In an applied magnetic field, the magnetization shows hysteresis loops with a distinct 'staircase' structure: the steps occur at values of the applied field where the energies of different collective spin states of the manganese clusters coincide. At these special values of the field, relaxation from one spin state to another is enhanced above the thermally activated rate by the action of resonant quantum-mechanical tunnelling. These observations corroborate the results of similar experiments performed recently on a system of oriented crystallites made from a powdered sample4.
1,542 citations
TL;DR: Direct evidence is presented indicating that mitochondrial PT constitutes a critical early event of the apoptotic process, and a specific ligand of the mitochondrial adenine nucleotide translocator, bongkreik acid, inhibits PT and reduces apoptosis induction by mitochondria in a cell-free system.
Abstract: Anucleate cells can be induced to undergo programmed cell death (PCD), indicating the existence of a cytoplasmic PCD pathway that functions independently from the nucleus. Cytoplasmic structures including mitochondria have been shown to participate in the control of apoptotic nuclear disintegration. Before cells exhibit common signs of nuclear apoptosis (chromatin condensation and endonuclease-mediated DNA fragmentation), they undergo a reduction of the mitochondrial transmembrane potential (delta psi m) that may be due to the opening of mitochondrial permeability transition (PT) pores. Here, we present direct evidence indicating that mitochondrial PT constitutes a critical early event of the apoptotic process. In a cell-free system combining purified mitochondria and nuclei, mitochondria undergoing PT suffice to induce chromatin condensation and DNA fragmentation. Induction of PT by pharmacological agents augments the apoptosis-inducing potential of mitochondria. In contrast, prevention of PT by pharmacological agents impedes nuclear apoptosis, both in vitro and in vivo. Mitochondria from hepatocytes or lymphoid cells undergoing apoptosis, but not those from normal cells, induce disintegration of isolated Hela nuclei. A specific ligand of the mitochondrial adenine nucleotide translocator (ANT), bongkreik acid, inhibits PT and reduces apoptosis induction by mitochondria in a cell-free system. Moreover, it inhibits the induction of apoptosis in intact cells. Several pieces of evidence suggest that the proto-oncogene product Bcl-2 inhibits apoptosis by preventing mitochondrial PT. First, to inhibit nuclear apoptosis, Bcl-2 must be localized in mitochondrial but not nuclear membranes. Second, transfection-enforced hyperexpression of Bcl-2 directly abolishes the induction of mitochondrial PT in response to a protonophore, a pro-oxidant, as well as to the ANT ligand atractyloside, correlating with its apoptosis-inhibitory effect. In conclusion, mitochondrial PT appears to be a critical step of the apoptotic cascade.
TL;DR: The power of FST-estimator tests and of allelic goodness offit tests are similar when sampling is balanced, and higher than the power of genotypic goodness of fit tests.
Abstract: We examine the power of different exact tests of differentiation for diploid populations. Since there is not necessarily random mating within populations, the appropriate hypothesis to construct exact tests is that of independent sampling of genotypes. There are two categories of tests, Fsrestimator tests and goodness of fit tests. In this latter category, we distinguish “allelic statistics”, which account for the nature of alleles within genotypes, from “genotypic statistics” that do not. We show that the power of Fs+stimator tests and of allelic goodness of fit tests are similar when sampling is balanced, and higher than the power of genotypic goodness of fit tests. When sampling is unbalanced, the most powerful tests are shown to belong to the allelic goodness of fit group.
TL;DR: The notion that MAPK cascades drive specific cell cycle responses to extracellular stimuli, at least in part, through the modulation of cyclin D1 expression and associated cdk activities is supported.
TL;DR: It is shown that mitochondria contain a pre-formed approximately 50-kD protein which is released upon delta psi m disruption and which, in a cell-free in vitro system, causes isolated nuclei to undergo apoptotic changes such as chromatin condensation and internucleosomal DNA fragmentation, and the effect of Bcl-2 on the formation, release, and action of AIF.
Abstract: Bcl-2 belongs to a family of apoptosis-regulatory proteins which incorporate into the outer mitochondrial as well as nuclear membranes. The mechanism by which the proto-oncogene product Bcl-2 inhibits apoptosis is thus far elusive. We and others have shown previously that the first biochemical alteration detectable in cells undergoing apoptosis, well before nuclear changes become manifest, is a collapse of the mitochondrial inner membrane potential (delta psi m), suggesting the involvement of mitochondrial products in the apoptotic cascade. Here we show that mitochondria contain a pre-formed approximately 50-kD protein which is released upon delta psi m disruption and which, in a cell-free in vitro system, causes isolated nuclei to undergo apoptotic changes such as chromatin condensation and internucleosomal DNA fragmentation. This apoptosis-inducing factor (AIF) is blocked by N-benzyloxycarbonyl-Val-Ala-Asp.fluoromethylketone (Z-VAD.fmk), an antagonist of interleukin-1 beta-converting enzyme (ICE)-like proteases that is also an efficient inhibitor of apoptosis in cells. We have tested the effect of Bcl-2 on the formation, release, and action of AIF. When preventing mitochondrial permeability transition (which accounts for the pre-apoptotic delta psi m disruption in cells), Bcl-2 hyperexpressed in the outer mitochondrial membrane also impedes the release of AIF from isolated mitochondria in vitro. In contrast, Bcl-2 does not affect the formation of AIF, which is contained in comparable quantities in control mitochondria and in mitochondria from Bcl-2-hyperexpressing cells. Furthermore, the presence of Bcl-2 in the nuclear membrane does not interfere with the action of AIF on the nucleus, nor does Bcl-2 hyperexpression protect cells against AIF. It thus appears that Bcl-2 prevents apoptosis by favoring the retention of an apoptogenic protease in mitochondria.
TL;DR: In this paper, the authors used the Canada-France Redshift Survey (CFRS) faint galaxy sample in three wave bands (2800 A, 4400 A and 1 μm) over the redshift range 0 < z < 1.
Abstract: The comoving luminosity density of the universe, (λ), is estimated from the Canada-France Redshift Survey (CFRS) faint galaxy sample in three wave bands (2800 A, 4400 A, and 1 μm) over the redshift range 0 < z < 1. In all three wave bands, increases markedly with redshift. For a (q0 = 0.5, Ω = 1.0) cosmological model, the comoving luminosity density increases as (1 + z)2.1 ± 0.5 at 1 μm, as (1 + z)2.7 ± 0.5 at 4400 A, and as (1 + z)3.9 ± 0.75 at 2800 A, these exponents being reduced by 0.43 and 1.12 for (0.05, 0.1) and (-0.85, 0.1) cosmological models, respectively. The (λ)-τ relation can be reasonably well modeled by an actively evolving stellar population with a Salpeter initial mass function (IMF) extending to 125 M☉, and a star formation rate declining as τ-2.5 with a turn-on of star formation at early epochs. A Scalo IMF extending to the same mass limit produces too many long-lived low-mass stars. This rapid evolution of the star formation rate and comoving luminosity density of the universe is in good agreement with the conclusions of Pei & Fall from their analysis of the evolving metallicity of the universe. One consequence of this evolution is that the physical luminosity density at short wavelengths has probably declined by 2 orders of magnitude since z ~ 1.
TL;DR: In this paper, the authors date fossil corals from Tahiti, which is far from plate boundaries and thus is likely to be tectonically relatively stable, and remote from the locations of large former ice sheets, and reveal that the meltwater pulse coincides with a short and intense climate cooling event that followed the initiation of the Bolling-Allerod warm period.
Abstract: THE timing of the last deglaciation is important to our understanding of the dynamics of large ice sheets1 and their effects on the Earth's surface2,3. Moreover, the disappearance of the glacial ice sheets was responsible for dramatic increases in freshwater fluxes to the oceans, which probably disturbed the ocean's thermohaline circulation and, hence, global climate4–7. Sea-level increases bear witness to the melting of continental ice sheets, but only two such records—from Barbados8,9 and New Guinea10,11 corals—have been accurately dated. But these corals overlie active subduction zones, where tectonic movements are large and often discontinuous (especially in New Guinea), so the apparent sea-level records may be contaminated by a complex tectonic component. Here we date fossil corals from Tahiti, which is far from plate boundaries (and thus is likely to be tectonically relatively stable) and remote from the locations of large former ice sheets. The resulting record indicates a large sea-level jump shortly before 13,800 calendar years BP, which corresponds to meltwater pulse 1A in the Barbados coral records8,9. The timing of this event is more accurately constrained in the Tahiti record, revealing that the meltwater pulse coincides with a short and intense climate cooling event12–15 that followed the initiation of the Bolling–Allerod warm period12–16, but preceded the Younger Dryas cold event by about 1,000 years.
National Institutes of Health1, Stanford University2, Massachusetts Institute of Technology3, Wellcome Trust Sanger Institute4, University of Oxford5, Wellcome Trust6, University of Michigan7, Rockefeller University8, Laval University9, J. Craig Venter Institute10, Centre national de la recherche scientifique11, Anschutz Medical Campus12, University of Cambridge13, McGill University14
TL;DR: The gene map unifies the existing genetic and physical maps with the nucleotide and protein sequence databases in a fashion that should speed the discovery of genes underlying inherited human disease.
Abstract: The human genome is thought to harbor 50,000 to 100,000 genes, of which about half have been sampled to date in the form of expressed sequence tags. An international consortium was organized to develop and map gene-based sequence tagged site markers on a set of two radiation hybrid panels and a yeast artificial chromosome library. More than 16,000 human genes have been mapped relative to a framework map that contains about 1000 polymorphic genetic markers. The gene map unifies the existing genetic and physical maps with the nucleotide and protein sequence databases in a fashion that should speed the discovery of genes underlying inherited human disease. The integrated resource is available through a site on the World Wide Web at http://www.ncbi.nlm.nih.gov/SCIENCE96/.
TL;DR: It is shown that the expression of MHC–I at the surface of lymphoid, monocytic and epithelial cells was reduced in the presence of Nef protein from various HIV–1 strains, representing a previously undocumented viral mechanism for evading the immune response.
Abstract: Like other pathogenic viruses, HIV-1 down-modulates surface expression of major histocompatibility complex class I (MHC-I) molecules in infected cells, thus impairing lysis by cytotoxic T lymphocytes. We have observed that this phenomenon depends on the expression of Nef. nef is an early gene of primate lentiviruses, which is necessary for maintaining high virus loads and inducing AIDS. Nef is not necessary for viral replication in vitro and stimulates the endocytosis of CD4. We show that the expression of MHC-I at the surface of lymphoid, monocytic and epithelial cells was reduced in the presence of Nef protein from various HIV-1 strains. Whereas MHC-I protein synthesis and transport through the endoplasmic reticulum and cis Golgi apparatus occurred normally in Nef(+) cells, surface MHC-I molecules were rapidly internalized, accumulated in endosomal vesicles and were degraded. The stimulation of MHC-I endocytosis by Nef represents a previously undocumented viral mechanism for evading the immune response.
TL;DR: A genome-wide search performed on two consecutive and independent panels of families with multiple affected members, using a non-parametric two-point sibling-pair linkage method, identified a putative CD-susceptibility locus on chromosome 16 which contains candidate genes which may be relevant to the pathogenic mechanism of inflammatory bowel diseases.
Abstract: Crohn's disease (CD) and ulcerative colitis are the major forms of chronic inflammatory bowel diseases in the western world, and occur in young adults with an estimated prevalence of more than one per thousand inhabitants. The causes of inflammatory bowel diseases remain unknown, but genetic epidemiology studies suggest that inherited factors may contribute in part to variation in individual susceptibility to Crohn's disease. A genome-wide search performed on two consecutive and independent panels of families with multiple affected members, using a non-parametric two-point sibling-pair linkage method, identified a putative CD-susceptibility locus on chromosome 16 (P less than 0.01 for each panel). The localization was centered around loci D16S409 and D16S419 by using multipoint sibpair analysis (P less than 1.5x10(-5)). This region of the genome contains candidate genes which may be relevant to the pathogenic mechanism of inflammatory bowel diseases.
TL;DR: In this article, an externalist and pragmatic conception of mind is proposed, which considers cognition as a social process and reintegrates it into the performance of situated actions, grasps performance as a genuine praxis and specifies the thinking and reflection which animate it in relation to the phenomenon of embodied agency.
Abstract: How may we conceive of cognition in practice? What kind of thinking and reflection animate the accomplishment of action? These problems are usually settled by an intellectualist argument: to perform an action is mainly to execute decisions, to carry out plans or intentions, or to follow instructions. According to that view, cognition produces action, but it does not take place in the accomplishment of action itself Such an intellectualist view has been taken up again and developed by recent trends in cognitive science. Why focus on such a view? Because, by its systematizing of current assumptions in most of (he theories of action, it makes the conceptual framework of those theories very clear and allows one to see the inconsistencies of its underpinning. The alternative view outlined in this paper is based on an externalist and pragmatic conception of mind. It considers cognition as a social process and reintegrates it into the performance of situated actions. To do so, it grasps performance as a genuine praxis and specifies the thinking and reflection which animate it in relation to the phenomenon of 'embodied agency.'
TL;DR: The force-displacement response of a single duplex DNA molecule was measured and a highly cooperative transition to a state here termed S-DNA is revealed, which yields a force plateau and suggests a structure for the extended form.
Abstract: The force-displacement response of a single duplex DNA molecule was measured. The force saturates at a plateau around 70 piconewtons, which ends when the DNA has been stretched about 1.7 times its contour length. This behavior reveals a highly cooperative transition to a state here termed S-DNA. Addition of an intercalator suppresses this transition. Molecular modeling of the process also yields a force plateau and suggests a structure for the extended form. These results may shed light on biological processes involving DNA extension and open the route for mechanical studies on individual molecules in a previously unexplored range.
TL;DR: In this article, Li and Haggans analyzed the convergence rate of the coupled-wave method for TM polarization of metallic lamellar gratings and provided numerical evidence that highly improved convergence rates similar to the TE polarization case can be obtained.
Abstract: The coupled-wave method formulated by Moharam and Gaylord [ J. Opt. Soc. Am.73, 451 ( 1983)] is known to be slowly converging, especially for TM polarization of metallic lamellar gratings. The slow convergence rate has been analyzed in detail by Li and Haggans [ J. Opt. Soc. Am. A10, 1184 ( 1993)], who made clear that special care must be taken when coupled-wave methods are used for TM polarization. By reformulating the eigenproblem of the coupled-wave method, we provide numerical evidence and argue that highly improved convergence rates similar to the TE polarization case can be obtained. The discussion includes both nonconical and conical mountings.
TL;DR: This review summarizes the current knowledge on the biosynthesis, structure, and function of this new class of signaling molecules and discusses the possibility that these signals could be part of a new family of plant lipo-chitooligosaccharide growth regulators.
Abstract: Rhizobia elicit on their specific leguminous hosts the formation of new organs, called nodules, in which they fix nitrogen. The rhizobia] nodulation genes specify the synthesis of lipo-chitooligosaccharide signals, the Nod factors (NFs). Each rhizobial species has a characteristic set of nodulation genes that specifies the length of the chitooligosaccharide backbone and the type of substitutions at both ends of the molecule, thus making the NFs specific for a given plant host. At extremely low concentrations, purified NFs are capable of eliciting on homologous legume hosts many of the plant developmental responses characteristic of the bacteria themselves, including cell divisions, and the triggering of a plant organogenic program. This review summarizes our current knowledge on the biosynthesis, structure, and function of this new class of signaling molecules. Finally we discuss the possibility that these signals could be part of a new family of plant lipochitooligosaccharide growth regulators.
TL;DR: The relationships among these estimators of population differentiation are discussed and how a single analysis of variance framework can accomodate these qualitatively different data types is shown.
Abstract: Several estimators of population differentiation have been proposed in the recent past to deal with various types of genetic markers (i.e., allozymes, nucleotide sequences, restriction fragment length polymorphisms, or microsatellites). We discuss the relationships among these estimators and show how a single analysis of variance framework can accomodate these qualitatively different data types.
TL;DR: Testing the effect of a specific inhibitor of PT, bongkrekic acid (BA), a ligand of the mitochondrial adenine nucleotide translocator, on a prototypic model of apoptosis glucocorticoid-induced thymocyte death suggests that a number of apoptotic phenomena are secondary to PT.
Abstract: In a number of experimental systems, the early stage of the apoptotic process, i.e., the stage that precedes nuclear disintegration, is characterized by the breakdown of the inner mitochondrial transmembrane potential (delta psi m). This delta psi m disruption is mediated by the opening of permeability transition (PT) pores and appears to be critical for the apoptotic cascade, since it is directly regulated by Bcl-2 and since mitochondria induced to undergo PT in vitro become capable of inducing nuclear chromatinolysis in a cell-free system of apoptosis. Here, we addressed the question of which apoptotic events are secondary to mitochondrial PT. We tested the effect of a specific inhibitor of PT, bongkrekic acid (BA), a ligand of the mitochondrial adenine nucleotide translocator, on a prototypic model of apoptosis glucocorticoid-induced thymocyte death. In addition to abolishing the apoptotic delta psi m disruption, BA prevents a number of phenomena linked to apoptosis: depletion of nonoxidized glutathione, generation of reactive oxygen species, translocation of NF kappa B, exposure of phosphatidylserine residues on the outer plasma membrane, cytoplasmic vacuolization, chromatin condensation, and oligonucleosomal DNA fragmentation. BA is also an efficient inhibitor of p53-dependent thymocyte apoptosis induced by DNA damage. These data suggest that a number of apoptotic phenomena are secondary to PT. In addition, we present data indicating that apoptotic delta psi m disruption is secondary to transcriptional events. These data connect the PT control point to the p53- and ICE/ Ced 3-regulated control points of apoptosis and place PT upstream of nuclear and plasma membrane features of PCD.
TL;DR: This work states that within a landscape, a permanent migration of propagules or individuals from source to sink habitats may lead to a stabilization of the overall demographic system, and this simple situation, explored in the recent literature, has surprising properties.
Abstract: The various habitats inhabited by a given species are never of the same quality. When demographic models take into account this habitat heterogeneity, the source-sink concept naturally emerges: a local demographic surplus arises in good quality habitats (source), and a local demographic deficit occurs in habitats of poor quality (sink). Within a landscape, a permanent migration of propagules or individuals from source to sink habitats may lead to a stabilization of the overall demographic system. This simple situation, explored in the recent literature, has surprising properties. In particular, it requires a change in our view of classical concepts such as ecological niche and carrying capacity, it can explain the existence and persistence of local maladaptation and it can improve conservation practice.
TL;DR: In this article, it is shown that phase objects can lead to spurious contrast in x-ray diffraction images (topographs) of crystals, which can be eliminated through random phase plates, which provide an effective way of tailoring the angular size of the source.
Abstract: Phase objects are readily imaged through Fresnel diffraction in the hard x-ray beams of third-generation synchrotron radiation sources such as the ESRF, due essentially to the very small angular size of the source. Phase objects can lead to spurious contrast in x-ray diffraction images (topographs) of crystals. It is shown that this contrast can be eliminated through random phase plates, which provide an effective way of tailoring the angular size of the source. The possibilities of this very simple technique for imaging phase objects in the hard x-ray range are explored experimentally and discussed. They appear very promising, as shown in particular by the example of a piece of human vertebra, and could be extended to phase tomography.
TL;DR: In this paper, the authors developed detailed emission inventories for the amount of both black and organic carbon particles from biomass burning sources (wood fuel, charcoal burning, dung, charcoal production, agricultural, savanna and forest fires).
Abstract: We have developed detailed emission inventories for the amount of both black and organic carbon particles from biomass burning sources (wood fuel, charcoal burning, dung, charcoal production, agricultural, savanna and forest fires). We have also estimated an inventory for organic carbon particles from fossil fuel burning and urban activities from an existing inventory for fossil fuel sources of black carbon. We also provide an estimate for the natural source of organic matter. These emissions have been used together with our global aerosol model to study the global distribution of carbonaceous aerosols. The accuracy of the inventories and the model formulation has been tested by comparing the model simulations of carbonaceous aerosols in the atmosphere and in precipitation with observations reported in the literature. For most locations and seasons, the predicted concentrations are in reasonable agreement with the observations, although the model underpredicts black carbon concentrations in polar regions. The predicted concentrations in remote areas are extremely sensitive to both the rate of removal by wet deposition and the height of injection of the aerosols. Finally, a global map of the aerosol single scattering albedo was developed from the simulated carbonaceous particle distribution and a previously developed model for aerosol sulfates. The computed aerosol single scattering albedos compare well with observations, suggesting that most of the important aerosol species have been included in the model. For most locations and seasons, the single scattering albedo is larger than 0.85, indicating that these aerosols, in general, lead to a net cooling.
TL;DR: In this paper, a new route to the successful arc-discharge synthesis of pure boron nitride (BN) nanotubes is presented, where carbon-free plasma is established between the electrodes in a nitrogen atmosphere.
Abstract: A new route to the successful arc-discharge synthesis of pure boron nitride (BN) nanotubes is presented. The carbon-free plasma is established between ${\mathrm{HfB}}_{2}$ electrodes in a nitrogen atmosphere. This technique leads to the formation of BN nanotubes with very few layers including single- and double-layer tubes. Electron-energy-loss spectroscopy yields a B:N ratio of approximately 1. Most tube ends are closed by flat layers perpendicular to the tube axis. A closure by a triangular facet, resulting from three 120\ifmmode^\circ\else\textdegree\fi{} disclinations, is proposed to account for this specific shape.