Showing papers by "Chaminade University of Honolulu published in 2002"
TL;DR: The preliminary work detailed here demonstrates, for the first time, the successful application of mtDNA sequencing techniques to the analysis of necrophagous beetle larvae, and demonstrates the robust nature of human mtDNA and the ability to recover valuable mtDNA evidence from forensically important, late decompositional stage insect species.
Abstract: The isolation, amplification, and characterization of human DNA from hematophagous (blood feeding) and necrophagous (carrion feeding) arthropods have been advanced significantly by the development of polymerase chain reaction (PCR) DNA sequencing methodologies. Historically, DNA technology has been successfully utilized to identify individual hosts upon which species of hematophagous arthropods have fed. The analysis of hematophagous insects' gut content blood meals has led to major advances in medical entomology and vector-borne disease epidemiology. In the forensic arena, the ability to apply similar techniques to insects recovered from badly decomposed remains has been greatly enhanced through the advent of mitochondrial DNA (mtDNA) techniques. Mitochondrial DNA analyses have been utilized to identify both the human remains upon which fly larvae (maggots) have fed and the species of the larvae themselves. The preliminary work detailed here demonstrates, for the first time, the successful application of mtDNA sequencing techniques to the analysis of necrophagous beetle larvae. A small sample of sap beetle larvae, Omosita spp. (Coleoptera: Nitidulidae), was collected from human skeletal remains during anthropological examination and analyzed for human DNA using mtDNA sequencing. The beetle larvae yielded mtDNA matching that of the host human bone. The results detailed here further demonstrate the robust nature of human mtDNA and the ability to recover valuable mtDNA evidence from forensically important, late decompositional stage insect species.
35 citations
15 Jul 2002
TL;DR: A phylogenetic analysis of Ilyscritonryia and members of the greenbottle genus Lueilia based on rnitochondrial cytochrome oxidase (COI+II) sequence data recovered the Lucilia species-groups that are supported by morphological criteria, and llyseritoayia was the sister lineage to Lucilia.
Abstract: greenbottle fiies, although the exact systematic position ofthe genus has never been known. This is part of a larger problem in that the limits and systematic position of many calliphorid species are unclear, and the monophyly of the Calliphoridae itself is in dispute. As part of a larger investigation of calliphorid systematics, we perfbrmed a phylogenetic analysis of Ilyscritonryia nd members of the greenbottle genus Lueilia based on rnitochondrial cytochrome oxidase (COI+II) sequence data, The resulting cladogram had }l87% bootstrap support for all branches and it recovered the Lucilia species-groups that are supported by morphological criteria, llyseritoayia was the sister lineage to Lucilia. Therefbre it is unlikely that the Hawaiian genus evolyed from any Lucilia speeies. Although these results are preliminary it appears that COI+II sequence data will provide a strong phylogenetic signal for any future investigation f Ilyscritonryia and other greenbottle flies.
18 citations
TL;DR: A new character of a nude tactile seta on femur III is noted for the genus and a key to the species of Polylopadium is presented.
Abstract: peruensis is described as new from specimens collected from Oryzomys yunganus, Proechimys quadruplicatus, and Oryzomys megacephalus taken in Peru. A new character of a nude tactile seta on femur III is noted for the genus and a key to the species of Polylopadium is presented.
1 citations