Chaminade University of Honolulu

About: Chaminade University of Honolulu is a education organization based out in Honolulu, Hawaii, United States. It is known for research contribution in the topics: Population & Experiential learning. The organization has 164 authors who have published 223 publications receiving 5381 citations.

Two-pore channel 1 interacts with citron kinase, regulating completion of cytokinesis.

Abstract: Two-pore channels (TPC1, 2, and 3) are recently identified endolysosmal ion channels, but remain poorly characterized. In this study, we show for the first time a role for TPC1 in cytokinesis, the final step in cell division. HEK 293 T-REx cells inducibly overexpressing TPC1 demonstrated a lack of proliferation accompanied by multinucleation and an increase in G2/M cycling cells. Increased TPC1 was associated with a concomitant accumulation of active RhoGTP and a decrease in phosphorylated myosin light chain (MLC). Finally, we demonstrated a novel interaction between TPC1 and citron kinase (CIT). These results identify TPC1 as a central component of cytokinetic control, specifically during abscission, and introduce a means by which the endolysosomal system may play an active role in this process.

Hirsutinolide Series Inhibit Stat3 Activity, Alter GCN1, MAP1B, Hsp105, G6PD, Vimentin, TrxR1, and Importin α-2 Expression, and Induce Antitumor Effects against Human Glioma.

Abstract: We report that hirsutinolide series, 6, 7, 10, 11, 20, and 22, and the semisynthetic analogues, 30, 31, 33, and 36, inhibit constitutively active signal transducer and activator of transcription (Stat)3 and malignant glioma phenotype. A position 13 lipophilic ester group is required for activity. Molecular modeling and nuclear magnetic resonance structural analyses reveal direct hirsutinolide:Stat3 binding. One-hour treatment of cells with 6 and 22 also upregulated importin subunit α-2 levels and repressed translational activator GCN1, microtubule-associated protein (MAP)1B, thioredoxin reductase (TrxR)1 cytoplasmic isoform 3, glucose-6-phosphate 1-dehydrogenase isoform a, Hsp105, vimentin, and tumor necrosis factor α-induced protein (TNAP)2 expression. Active hirsutinolides inhibited anchorage-dependent and three-dimensional spheroid growth, survival, and migration of human glioma lines and glioma patients’ tumor-derived xenograft cells harboring constitutively active Stat3. Oral gavage delivery of 6 or ...

Volatile Organic Compound Profiling from Postmortem Microbes using Gas Chromatography–Mass Spectrometry

Abstract: Volatile organic compounds (VOCs) are by-products of cadaveric decomposition and are responsible for the odor associated with decomposing remains. The direct link between VOC production and individual postmortem microbes has not been well characterized experimentally. The purpose of this study was to profile VOCs released from three postmortem bacterial isolates (Bacillus subtilis, Ignatzschineria indica, I. ureiclastica) using solid-phase microextraction arrow (SPME Arrow) and gas chromatography-mass spectrometry (GC-MS). Species were inoculated in headspace vials on Standard Nutrient Agar and monitored over 5 days at 24°C. Each species exhibited a different VOC profile that included common decomposition VOCs. VOCs exhibited upward or downward temporal trends over time. Ignatzschineria indica produced a large amount of dimethyldisulfide. Other compounds of interest included alcohols, aldehydes, aromatics, and ketones. This provides foundational data to link decomposition odor with specific postmortem microbes to improve understanding of underlying mechanisms for decomposition VOC production.

The political economic logic of RMB internationalization: A study in Sino-capitalism

Abstract: Over the past 7 years the Chinese government has begun in earnest a gradual process of seeking a more internationalized currency. By conceptualizing China’s domestic political economy as having generated Sino-capitalism, this article utilizes a ‘second image’ explanation to elucidate the logic of RMB internationalization policies. Therefore, rather than focusing on international incentives and constraints as they affect RMB internationalization the aim is to trace the origins of China’s unique policy stance with historical institutional analysis. The article begins with conceptualizing the domestic logic of Sino-capitalism and then highlights key aspects of Sino-capitalism’s institutional arrangements and policy approaches as they shape RMB internationalization. The article ends with arguing that the basic institutional logic and policy stances of Sino-capitalism are here to stay. With regard to RMB internationalization this indicates that full-scale liberalization measures, especially of China’s capital account, are unlikely to be undertaken in the near future. However, Sino-capitalism’s logic opens up new avenues by which China, as it becomes an ever larger part of the global political economy, could gradually foster an international currency.