Charles University in Prague

About: Charles University in Prague is a education organization based out in Prague, Czechia. It is known for research contribution in the topics: Population & Large Hadron Collider. The organization has 32392 authors who have published 74435 publications receiving 1804208 citations.

HPV Involvement in Head and Neck Cancers: Comprehensive Assessment of Biomarkers in 3680 Patients

Abstract: BACKGROUND: We conducted a large international study to estimate fractions of head and neck cancers (HNCs) attributable to human papillomavirus (HPV-AFs) using six HPV-related biomarkers of viral detection, transcription, and cellular transformation. METHODS: Formalin-fixed, paraffin-embedded cancer tissues of the oral cavity (OC), pharynx, and larynx were collected from pathology archives in 29 countries. All samples were subject to histopathological evaluation, DNA quality control, and HPV-DNA detection. Samples containing HPV-DNA were further subject to HPV E6*I mRNA detection and to p16(INK4a), pRb, p53, and Cyclin D1 immunohistochemistry. Final estimates of HPV-AFs were based on HPV-DNA, HPV E6*I mRNA, and/or p16(INK4a) results. RESULTS: A total of 3680 samples yielded valid results: 1374 pharyngeal, 1264 OC, and 1042 laryngeal cancers. HPV-AF estimates based on positivity for HPV-DNA, and for either HPV E6*I mRNA or p16(INK4a), were 22.4%, 4.4%, and 3.5% for cancers of the oropharynx, OC, and larynx, respectively, and 18.5%, 3.0%, and 1.5% when requiring simultaneous positivity for all three markers. HPV16 was largely the most common type. Estimates of HPV-AF in the oropharynx were highest in South America, Central and Eastern Europe, and Northern Europe, and lowest in Southern Europe. Women showed higher HPV-AFs than men for cancers of the oropharynx in Europe and for the larynx in Central-South America. CONCLUSIONS: HPV contribution to HNCs is substantial but highly heterogeneous by cancer site, region, and sex. This study, the largest exploring HPV attribution in HNCs, confirms the important role of HPVs in oropharyngeal cancer and drastically downplays the previously reported involvement of HPVs in the other HNCs.

Computation of wave fields in inhomogeneous media — Gaussian beam approach

Abstract: Summary. An asymptotic procedure for the computation of wave fields in two-dimensional laterally inhomogeneous media is proposed. It is based on the simulation of the wave field by a system of Gaussian beams. Each beam is continued independently through an arbitrary inhomogeneous structure. The complete wave field at a receiver is then obtained as an integral superposition of all Gaussian beams arriving in some neighbourhood of the receiver. The corresponding integral formula is valid even in various singular regions where the ray method fails (the vicinity of caustic, critical point, etc.). Numerical examples are given.

Singular value decomposition methods for wave propagation analysis

Abstract: [1] We describe several newly developed methods for propagation analysis of electromagnetic plasma waves. We make use of singular value decomposition (SVD) technique and we determine the wave vector direction, ellipticity and directions of axes of the polarization ellipse, wave refractive index, transfer function of electric antennas, estimators of the planarity of polarization, and electromagnetic planarity. Simulations of Z-mode waves, which simultaneously propagate with different wave vectors, indicate that the SVD methods give reasonable results even if the assumption on the presence of a single plane wave is invalid. Simulations of whistler mode waves show that these methods can be used to recognize cases when the waves simultaneously propagate with wave vectors in two opposite hemispheres. Finally, we show an example of analysis of natural whistler mode and Z-mode emissions measured in the high-altitude auroral region by the MEMO experiment onboard the INTERBALL spacecraft.