Showing papers by "Chung-Ang University published in 1978"
TL;DR: The results suggest that clonorchiasis could be a factor in inducing primary carcinoma of liver in albino rats.
Abstract: The present study was undertaken to observe the pathologic changes of the liver in albino rats with Clonorchis sinensis. Twenty five rats given 50 metacercariae respectively by mouth were autopsied at 3 days (group 1), one week (group 2), 4 weeks (group 3), 8 weeks (group 4) and 12 weeks (group 5) of infection. The following results were obtained: 1. Parasites were seen in bile ducts of group 2, 3, 4, 5 and increased in number with duration of infection. 2. The pathologic changes in the liver were prominent in intrahepatic bile ducts. Epithelial cells of bile ducts showed definite atypia and proliferation resulting in pseudostratification in group. Stratification of metaplastic squamous cells and glandular proliferation were prominent in group 3. The epithelial cells were keratinized with syncitium and cribriform formation in group 4 and almost suggestive of adenomatous hyperplasia in group 5. 3. Periductal fibrosis seen in group 4 was considerable as well as mature with hyalinized connective tissue in group 5. 4. Heavy inflammatory cell infiltrations around the affected bile ducts in group 1 became smaller in number with duration of infection. The inflammatory cells consisted of the majority of eosinophils in group 1 and chiefly plasma cells, lymphocytes and mononuclear cells in group 5. 5. Veins in portal spaces dilated markedly in group 1 became less prominent with duration of infection. 6. Although portal fibrosis increased definitely in group 3 often extended into the hepatic lobules in group 4, the changes of hepatic cells, sinusoids and central veins were negligible. Above results suggest that clonorchiasis could be a factor in inducing primary carcinoma of liver in albino rats.
34 citations
TL;DR: It is suggested that oxantel-pyrantel is more effective in the treatment of mixed infections of Ascaris and Trichuris, and was more acceptable to the patients, than mebendazole.
Abstract: A comparative trial was carried out in 4 orphanages in Seoul, to compare the anthelminthic efficacy of oxantel-pyrantel suspension and mebendazole tablets against mixed infections of Ascaris lumbricoides and Trichuris trichiura.
12 citations
TL;DR: The present study was undertaken to observe the changes of hepatocyte and biliary epithelium in the liver of guinea pigs infected with C. sinensis by light and electronmicroscopic observation of these tissue preparations.
Abstract: The present study was undertaken to observe the changes of hepatocyte and biliary epithelium in the liver of guinea pigs infected with C. sinensis. Ten guinea pigs infected with 500 metacercariae of C. sinensis each were reared for 11 weeks. After sacrifice, the liver tissues were processed both for conventional light microscopy and for electronmicroscopy. The following results were obtained by light and electronmicroscopic observation of these tissue preparations. 1. The dilatation of bile ducts with chronic inflammatory cell infiltration and fibrosis, and flattening of lining epithelium were observed by light microscopy. Bile duct showed a single low columnar or cuboidal epithelium and subepithelial small round cell infiltration. Papillary proliferation, adenomatous hyperplasia and increase of goblet cells in the subepithelial glands were also noted in some areas of bile ducts. Occasionally, small fibrous nodules in the portal tract replacing bile ducts, surrounded by a heavy infiltrate of small round cells and neutrophils were observed. 2. On light microscopic examination, no cellular damages were seen in the liver cells except mild fatty degeneration. 3. Electronmicroscopic examination of the biliary epithelium revealed increased mucin granules, cytoplasmic projection into lumen, decreased microvilli and obstruction of bile canaliculi. Indistinct and/or irregular appearances of intercellular lateral interdigitation were observed in most of biliary epithelium. 4. On the electronmicrographs of hepatocytes, dilation of endoplasmic reticulum(ER) and destruction of cristae in some mitochondriae were prominent features. Disappearance of cytoplasmic organells and dilatation of bile canaliculi were also monitored. 5. Endothelial cells in the sinusoids were remained intact.
9 citations
TL;DR: It was found that histone H2b fraction of Fasciola hepatica contained detectable amounts of epsilon-N-monomethyllysine, and no evidence for the presence of methylated lysine or other side-chain derivatives was reported on this histone fraction.
Abstract: In attempt to investigate histone fractions and non-histones of parasites, nuclei were isolated from Fasciola hepatica by the procedure of Pogo et al (1966) Histone fractions H1, H2a, H2b, H3 and H4 were prepared from isolated nuclei by the procedure of Johns (1964 and l967) The five histone fractions found in most tissues were also present in the Fasciola hepatica histones These histone fractions were characterized by amino acid analysis and by polyacrylamide disc gel electrophoresis Non-histone proteins were extracted from isolated Fasciola hepatica nuclei and separated by SDS-polyacrylamide gel electrophoresis The results of the experiment were summarized as follows: 1 The yield of whole histone recovered was 247 mg per 1 g of Fasciola hepatica 2 The yield of DNA was 102 mg per gm of tissues Consequently the DNA to histone ratio was 1:244 3 The relative amounts of five fractions, ie, Hl, H2a, H2b, H3 and H4 were 1996%, 2648%, 2960%, 1256% and 1437%, respectively 4 Amino acid analysis of the individual histone fractions showed that the over-all compositions were similar but not identical to those of corresponding fraction from calf thymus 5 It was found that histone H2b fraction of Fasciola hepatica contained detectable amounts of epsilon-N-monomethyllysine No evidence for the presence of methylated lysine or other side-chain derivatives was reported on this histone fraction 6 In SDS-polyacrylamide disc gel, it showed that 17 protein bands of nuclear acidic protein can be identified visually
1 citations
TL;DR: The recovery rate and survival rate of the worms decreased and the worms recovered in the capsule of the liver were degenerated and necrotized, Usually, there were remarkable capsulitis and granuloma formation around the eggs.
Abstract: In order to understand the effect of prednisolone injection on the histopathological changes of the mouse liver and the chronological changes of the worm structure of Clonorchis sinensis, when this fluke was inoculated to the mouse intraperitoneally. The recovery rate, survival rate, location and size of the inoculated worms as well as the histopathological changes of the liver were investigated for the comparison among the groups of mice, which were classified by number of worms and the duration of observation period. The result obtained were summarized as follows. 1. The recovery rate and survival rate of the worms decreased especially 28 days after the inoculation. 2. Most of worms (45.5%) were collected from the peritoneal cavity, and some of worms were found tightly adherent to the capsules of the liver, spleen, intestine and diaphragm. 3. The mean worm size after inoculation was constantly smaller than that before inoculation. 4. Remarkable atrophy in the reproductive organs of the worm, such as spermatheca, testes, vitelline gland and ovary was frequently observed at the 10th day of inoculation. 5. Histopathologically the liver failed to show any parasitic worm inside the intrahepatic biliary system. However, multiple well formed egg-containing granulomas were present along the liver capsule. These necrotic granulomas were occasionally found under the fibrotic liver capsule. Focal necrosis and focal phlebitis together with vascular dilatation were prominent features of the liver. 6. The worms recovered in the capsule of the liver were degenerated and necrotized. Usually, there were remarkable capsulitis and granuloma formation around the eggs.