Chung Yuan Christian University

About: Chung Yuan Christian University is a education organization based out in Taoyuan City, Taiwan. It is known for research contribution in the topics: Membrane & Fuzzy logic. The organization has 9819 authors who have published 11623 publications receiving 213139 citations. The organization is also known as: Tiong-gôan-tāi-ha̍k & CYCU.

Microcalorimetrics studies of the thermodynamics and binding mechanism between L-tyrosinamide and aptamer.

Abstract: In recent years, several high-resolution structures of aptamer complexes have shed light on the binding mode and recognition principles of aptamer complex interactions. In some cases, however, the aptamer complex binding behavior and mechanism are not clearly understood, especially with the absence of structural information. In this study, it was demonstrated that isothermal titration calorimetry (ITC) and circular dichroism (CD) were useful tools for studying the fundamental binding mechanism between a DNA aptamer and L-tyrosinamide (L-TyrNH2). To gain further insight into this behavior, thermodynamic and conformational measurements under different parameters such as salt concentration, temperature, pH value, analogue of L-TyrNH2, and metal ion were carried out. The thermodynamic signature along with the coupled CD spectral change suggest that this binding behavior is an enthalpy-driven process, and the aptamer has a conformational change from B-form to A-form. The results showed that the interaction is an induced fit binding, and the driving forces in this binding behavior may include electrostatic interactions, hydrophobic effects, hydrogen bonding, and the binding-linked protonation process. The amide group and phenolic hydroxyl group of the L-TyrNH2 play a vital role in this binding mechanism. In addition, it should be noted that Mg(2+) not only improves binding affinity but also helps change the structure of the DNA aptamer.

Study on the sensing characteristics and hysteresis effect of the tin oxide pH electrode

Abstract: Tin oxide thin films prepared by the radio frequency sputtering method were used as pH sensors based on the structure of the metal oxide electrode. The sensing characteristics and the hysteresis effect of the tin oxide pH electrode were studied. The hysteresis experiments were carried out under short- and long-loop circles, pH 7–4–7–10–7 and pH 7–2–7–12–6, respectively. Moreover, the hysteresis value was less than 7 mV in short-loop circle and the hysteresis value of pH 7 was 9 mV in acid side ( H A ) and 11 mV in alkali side ( H B ). The sensitivity of the tin oxide pH electrode was investigated between pH 2 and 12, whose pH sensitivity was about 59 mV/pH.