Institution
Colorado State University
Education•Fort Collins, Colorado, United States•
About: Colorado State University is a education organization based out in Fort Collins, Colorado, United States. It is known for research contribution in the topics: Population & Radar. The organization has 31430 authors who have published 69040 publications receiving 2724463 citations. The organization is also known as: CSU & Colorado Agricultural College.
Topics: Population, Radar, Poison control, Laser, Soil water
Papers published on a yearly basis
Papers
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TL;DR: This study is the first to demonstrate a relationship between urban and agricultural activity and microbial resistance in river sediments using quantitative molecular tools.
702 citations
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TL;DR: The Tn7 system allows engineering of diverse genetic traits into bacteria, as demonstrated by complementing a biofilm-growth defect of P. aeruginosa, establishing expression systems in P. putida and Yersinia pestis, and 'GFP-tagging' Y. pestis.
Abstract: For many bacteria, cloning and expression systems are either scarce or nonexistent. We constructed several mini-Tn7 vectors and evaluated their potential as broad-range cloning and expression systems. In bacteria with a single chromosome, including Pseudomonas aeruginosa, Pseudomonas putida and Yersinia pestis, and in the presence of a helper plasmid encoding the site-specific transposition pathway, site- and orientation-specific Tn7 insertions occurred at a single attTn7 site downstream of the glmS gene. Burkholderia thailandensis contains two chromosomes, each containing a glmS gene and an attTn7 site. The Tn7 system allows engineering of diverse genetic traits into bacteria, as demonstrated by complementing a biofilm-growth defect of P. aeruginosa, establishing expression systems in P. aeruginosa and P. putida, and 'GFP-tagging' Y. pestis. This system will thus have widespread biomedical and environmental applications, especially in environments where plasmids and antibiotic selection are not feasible, namely in plant and animal models or biofilms.
702 citations
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TL;DR: The environmental impacts of the nuclear accidents of Chernobyl and Fukushima are compared and monitoring campaigns after both accidents reveal that the environmental impact of the Chernobyl accident was much greater than of the Fukushima accident.
701 citations
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TL;DR: In contrast to existing chromosome integration systems, which are mostly based on species-specific phage or more-or-less randomly integrating transposons, the mini-Tn7 system is characterized by its ready adaptability to various bacterial hosts, its site specificity and its efficiency.
Abstract: Broad host-range mini-Tn7 vectors facilitate integration of single-copy genes into bacterial chromosomes at a neutral, naturally evolved site. Here we present a protocol for employing the mini-Tn7 system in bacteria with single attTn7 sites, using the example Pseudomonas aeruginosa. The procedure involves, first, cloning of the genes of interest into an appropriate mini-Tn7 vector; second, co-transfer of the recombinant mini-Tn7 vector and a helper plasmid encoding the Tn7 site-specific transposition pathway into P. aeruginosa by either transformation or conjugation, followed by selection of insertion-containing strains; third, PCR verification of mini-Tn7 insertions; and last, optional Flp-mediated excision of the antibiotic-resistance selection marker present on the chromosomally integrated mini-Tn7 element. From start to verification of the insertion events, the procedure takes as little as 4 d and is very efficient, yielding several thousand transformants per microgram of input DNA or conjugation mixture. In contrast to existing chromosome integration systems, which are mostly based on species-specific phage or more-or-less randomly integrating transposons, the mini-Tn7 system is characterized by its ready adaptability to various bacterial hosts, its site specificity and its efficiency. Vectors have been developed for gene complementation, construction of gene fusions, regulated gene expression and reporter gene tagging.
701 citations
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TL;DR: The cloning strategies for the construction of plasmids containing multiple repeats of defined DNA sequences, and the subsequent large-scale isolation of defined sequence DNA for nucleosome reconstitution are described.
Abstract: Publisher Summary The ability to prepare nucleosome core particles (NCPs), or nucleosomal arrays, from recombinant histone proteins and defined-sequence DNA has become a requirement in many projects that address the role of histone modifications, histone variants, or histone mutations in nucleosome and chromatin structure. The cloning strategies for the construction of plasmids containing multiple repeats of defined DNA sequences, and the subsequent large-scale isolation of defined sequence DNA for nucleosome reconstitution are described. This chapter also describes adapted procedures to prepare nucleosomes with histones from other species, and for the refolding and reconstitution of (H2A– H2B) dimers and (H3–H4) 2 tetramers. Methods to reconstitute nucleosomes from different histone subcomplexes are described. A flow chart for all procedures involved in the preparation of synthetic nucleosomes is also presented.
700 citations
Authors
Showing all 31766 results
Name | H-index | Papers | Citations |
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Mark P. Mattson | 200 | 980 | 138033 |
Stephen J. O'Brien | 153 | 1062 | 93025 |
Ad Bax | 138 | 486 | 97112 |
David Price | 138 | 1687 | 93535 |
Georgios B. Giannakis | 137 | 1321 | 73517 |
James Mueller | 134 | 1194 | 87738 |
Christopher B. Field | 133 | 408 | 88930 |
Steven W. Running | 126 | 355 | 76265 |
Simon Lin | 126 | 754 | 69084 |
Jitender P. Dubey | 124 | 1344 | 77275 |
Gregory P. Asner | 123 | 613 | 60547 |
Steven P. DenBaars | 118 | 1366 | 60343 |
Peter Molnar | 118 | 446 | 53480 |
William R. Jacobs | 118 | 490 | 48638 |
C. Patrignani | 117 | 1754 | 110008 |