Showing papers by "Cooperative Research Centre published in 1998"

Influence of lime, fertilizer and manure applications on soil organic matter content and soil physical conditions: A review

Abstract: The effects of lime, fertilizer and manure applications on soil organic matter status and soil physical properties are of importance to agricultural sustainability. Their effects are complex and many interactions can occur. In the short-term, liming can result in dispersion of clay colloids and formation of surface crusts. As pH is increased the surface negative charge on clay colloids increases and repulsive forces between particles dominate. However, at higher lime rates, Ca2+ concentrations and ionic strength in soil solution increase causing compression of the electrical double layer and renewed flocculation. When present in sufficient quantities, both lime and hydroxy-Al polymers formed by precipitation of exchangeable Al, can act as cementing agents bonding soil particles together and improving soil structure. Liming often causes a temporary flush of soil microbial activity but the effect of this on soil aggregation is unclear. It is suggested that, in the long-term, liming will increase crop yields, organic matter returns, soil organic matter content and thus soil aggregation. There is a need to study these relationships on existing long-term liming trials. Fertilizers are applied to soils in order to maintain or improve crop yields. In the long-term, increased crop yields and organic matter returns with regular fertilizer applications result in a higher soil organic matter content and biological activity being attained than where no fertilizers are applied. As a result, long-term fertilizer applications have been reported, in a number of cases, to cause increases in water stable aggregation, porosity, infiltration capacity and hydraulic conductivity and decreases in bulk density. Fertilizer additions can also have physico-chemical effects which influence soil aggregation. Phosphatic fertilizers and phosphoric acid can favour aggregation by the formation of Al or Ca phosphate binding agents whilst where fertilizer NH4 + accumulates in the soil at high concentrations, dispersion of clay colloids can be favoured. Additions of organic manures result in increased soil organic matter content. Many reports have shown that this results in increased water holding capacity, porosity, infiltration capacity, hydraulic conductivity and water stable aggregation and decreased bulk density and surface crusting. Problems associated with large applications of manure include dispersion caused by accumulated K+, Na+ and NH4 + in the soil and production of water-repellant substances by decomposer fungi.

Biodiversity inventories, indicator taxa and effects of habitat modification in tropical forest

Abstract: Despite concern about the effects of tropical forest disturbance and clearance on biodiversity1,2, data on impacts, particularly on invertebrates, remain scarce3,4,5,6,7,8. Here we report a taxonomically diverse inventory on the impacts of tropical forest modification at one locality. We examined a gradient from near-primary, through old-growth secondary and plantation forests to complete clearance, for eight animal groups (birds, butterflies, flying beetles, canopy beetles, canopy ants, leaf-litter ants, termites and soil nematodes) in the Mbalmayo Forest Reserve, south-central Cameroon. Although species richness generally declined with increasing disturbance, no one group serves as a good indicator taxon9,10,11,12 for changes in the species richness of other groups. Species replacement from site to site (turnover) along the gradient also differs between taxonomic groups. The proportion of ‘morphospecies’ that cannot be assigned to named species and the number of ‘scientist-hours’ required to process samples both increase dramatically for smaller-bodied taxa. Data from these eight groups indicate the huge scale of the biological effort required to provide inventories of tropical diversity, and to measure the impacts of tropical forest modification and clearance.

Biological control of weeds

Abstract: Classical biological control, i.e. the introduction and release of exotic insects, mites, or pathogens to give permanent control, is the predominant method in weed biocontrol. Inundative releases of predators and integrated pest management are less widely used. The United States, Australia, South Africa, Canada, and New Zealand use biocontrol the most. Weeds in natural ecosystems are increasingly becoming targets for biocontrol. Discussion continues on agent selection, but host-specificity testing is well developed and reliable. Post-release evaluation of impact is increasing, both on the target weed and on non-target plants. Control of aquatic weeds has been a notable success. Alien plant problems are increasing worldwide, and biocontrol offers the only safe, economic, and environmentally sustainable solution.

Mantle convection with a brittle lithosphere: thoughts on the global tectonic styles of the Earth and Venus

Abstract: SUMMARY Plates are an integral part of the convection system in the fluid mantle, but plate boundaries are the product of brittle faulting and plate motions are strongly influenced by the existence of such faults. The conditions for plate tectonics are studied by considering brittle behaviour, using Byerlee’s law to limit the maximum stress in the lithosphere, in a mantle convection model with temperature-dependent viscosity. When the yield stress is high, convection is confined below a thick, stagnant lithosphere. At low yield stress, brittle deformation mobilizes the lithosphere which becomes a part of the overall circulation; surface deformation occurs in localized regions close to upwellings and downwellings in the system. At intermediate levels of the yield stress, there is a cycling between these two states: thick lithosphere episodically mobilizes and collapses into the interior before reforming. The mobile-lid regime resembles convection of a fluid with temperature-dependent viscosity and the boundary-layer scalings are found to be analogous. This regime has a well defined Nusselt number‐Rayleigh number relationship which is in good agreement with scaling theory. The surface velocity is nearly independent of the yield stress, indicating that the ‘plate’ motion is resisted by viscous stresses in the mantle. Analysis suggests that mobilization of the Earth’s lithosphere can occur if the friction coeYcient in the lithosphere is less than 0.03‐0.13—lower than laboratory values but consistent with seismic field studies. On Venus, the friction coeYcient may be high as a result of the dry conditions, and brittle mobilization of the lithosphere would then be episodic and catastrophic.

Infertility in female mice lacking the receptor for interleukin 11 is due to a defective uterine response to implantation

Abstract: During early pregnancy, in response to the implanting embryo, the surrounding uterine stroma undergoes a dramatic transformation into a specialized tissue known as the decidua. The de-cidua encapsulates the developing embryo, facilitating nutrient transfer and limiting tro-phoblast invasion. Here we show that female mice with a null mutation of the interleukin-11 receptor alpha chain are infertile because of defective decidualization. A temporal analysis revealed IL-11 expression is maximal in the normal pregnant uterus at the time of decidualization, and in situ hybridization studies showed expression of the IL-11 and the IL-11 receptor alpha chain in the developing decidual cells. These observations reveal a previously unrecognized critical role for IL-11 signaling in female reproduction.

Beetle species responses to tropical forest fragmentation

Abstract: The effects of forest fragmentation on beetle species composition were investigated in an experimentally fragmented tropical forest landscape in Central Amazonia. Leaf-litter beetles were sampled at seven distances from the forest edge (0–420 m) along forest edge-to-interior transects in two 100-ha forest fragments and two continuous forest edges, and at an identical series of distances along two deep continuous forest transects. Additional samples were taken at the centers of two 10-ha forest fragments and two 1-ha fragments. This sampling regime allowed discrimination between edge and fragment area effects. Beetle species composition changed significantly and independently with both decreasing distance from forest edge and decreasing fragment area. Edge effects on species composition were mediated by six important environmental variables: air temperature, canopy height, percent ground cover of twigs, litter biomass, litter moisture content, and an air temperature × distance from edge interaction effect, due to the different temperature profiles of edges with differing edge vegetation density. Population densities of 15 of the 32 most abundant beetle species tested (47%) were significantly affected by forest fragmentation. Species responses were classified empirically into four major categories: (A) edge sensitive, area insensitive; (B) area sensitive, edge insensitive; (C) edge and area sensitive; and (D) edge and area insensitive. Within these categories, trends in density were either positive (deep-forest species), or negative (disturbed-area species), with species showing the full spectrum of responses to fragmentation. The vast majority of species were adversely affected. Estimated species loss rates from forest fragments were: 49.8% of common species from 1-ha fragments, 29.8% from 10-ha fragments, and 13.8% from 100-ha fragments. Declining density was a significant precursor of species loss from forest fragments, but other species that did not show significant population density responses to fragmentation were also absent from some fragments, presumably by chance. The probability of species loss from forest fragments was not correlated with body size or trophic group for the 32 common species, although for the entire beetle assemblage (993 species) proportions of species in different trophic groups changed significantly with fragmentation. Rarity and population variability (in undisturbed forest) were significant predictors of susceptibility to fragmentation. Surprisingly, though, common species were significantly more likely to become locally extinct in small fragments than rarer species. This lends empirical support to models of multispecies coexistence under disturbance that suggest competitively dominant but poorly dispersing species are the first to become extinct due to habitat destruction. Thus, rarer species are predicted to be better dispersers and better at persisting.

Dna methylation in plants

Abstract: Methylation of cytosine residues in DNA provides a mechanism of gene control. There are two classes of methyltransferase in Arabidopsis; one has a carboxy-terminal methyltransferase domain fused to an amino-terminal regulatory domain and is similar to mammalian methyltransferases. The second class apparently lacks an amino-terminal domain and is less well conserved. Methylcytosine can occur at any cytosine residue, but it is likely that clonal transmission of methylation patterns only occurs for cytosines in strand-symmetrical sequences CpG and CpNpG. In plants, as in mammals, DNA methylation has dual roles in defense against invading DNA and transposable elements and in gene regulation. Although originally reported as having no phenotypic consequence, reduced DNA methylation disrupts normal plant development.

Tethered Lipid Bilayer Membranes: Formation and Ionic Reservoir Characterization

Abstract: Using novel synthetic lipids, a tethered bilayer membrane (tBLM) was formed onto a gold electrode such that a well-defined ionic reservoir exists between the gold surface and the bilayer membrane. Self-assembled monolayers of reservoir-forming lipids were first adsorbed onto the gold surface using gold−sulfur interactions, followed by the formation of the tBLM using the self-assembly properties of phosphatidylcholine-based lipids in aqueous solution. The properties of the tBLM were investigated by impedance spectroscopy. The capacitance of the tBLM indicated the formation of bilayer membranes of comparable thickness to solvent-free black (or bilayer) lipid membranes (BLM). The ionic sealing ability was comparable to those of classical BLMs. The function of the ionic reservoir was investigated using the potassium-specific ionophore valinomycin. Increasing the size of the reservoir by increasing the length of the hydrophilic region of the reservoir lipid or laterally spacing the reservoir lipid results in a...

Algicidal Effects of a Novel Marine Pseudoalteromonas Isolate (Class Proteobacteria, Gamma Subdivision) on Harmful Algal Bloom Species of the Genera Chattonella, Gymnodinium, and Heterosigma

Abstract: During a bacterial survey of the Huon Estuary in southern Tasmania, Australia, we isolated a yellow-pigmented Pseudoalteromonas strain (class Proteobacteria, gamma subdivision), designated strain Y, that had potent algicidal effects on harmful algal bloom species. This organism was identified by 16S rRNA sequencing as a strain with close affinities to Pseudoalteromonas peptidysin. This bacterium caused rapid cell lysis and death (within 3 h) of gymnodinoids (including Gymnodinium catenatum) and raphidophytes (Chattonella marina and Heterosigma akashiwo). It caused ecdysis of armored dinoflagellates (e.g., Alexandrium catenella, Alexandrium minutum, and Prorocentrum mexicanum), but the algal cultures then recovered over the subsequent 24 h. Strain Y had no effect on a cryptomonad (Chroomonas sp.), a diatom (Skeletonema sp.), a cyanobacterium (Oscillatoria sp.), and two aplastidic protozoans. The algicidal principle of strain Y was excreted into the seawater medium and lost its efficacy after heating. Another common bacterial species, Pseudoalteromonas carrageenovora, was isolated at the same time and did not have these algicidal effects. The minimum concentrations of strain Y required to kill G. catenatum were higher than the mean concentrations found in nature under nonbloom conditions. However, the new bacterium showed a chemotactic, swarming behavior that resulted in localized high concentrations around target organisms. These observations imply that certain bacteria could play an important role in regulating the onset and development of harmful algal blooms.

Changes in Cell Wall Composition during Ripening of Grape Berries

Abstract: Cell walls were isolated from the mesocarp of grape (Vitis vinifera L.) berries at developmental stages from before veraison through to the final ripe berry. Fluorescence and light microscopy of intact berries revealed no measurable change in cell wall thickness as the mesocarp cells expanded in the ripening fruit. Isolated walls were analyzed for their protein contents and amino acid compositions, and for changes in the composition and solubility of constituent polysaccharides during development. Increases in protein content after veraison were accompanied by an approximate 3-fold increase in hydroxyproline content. The type I arabinogalactan content of the pectic polysaccharides decreased from approximately 20 mol % of total wall polysaccharides to about 4 mol % of wall polysaccharides during berry development. Galacturonan content increased from 26 to 41 mol % of wall polysaccharides, and the galacturonan appeared to become more soluble as ripening progressed. After an initial decrease in the degree of esterification of pectic polysaccharides, no further changes were observed nor were there large variations in cellulose (30–35 mol % of wall polysaccharides) or xyloglucan (approximately 10 mol % of wall polysaccharides) contents. Overall, the results indicate that no major changes in cell wall polysaccharide composition occurred during softening of ripening grape berries, but that significant modification of specific polysaccharide components were observed, together with large changes in protein composition.

Positive-definite Toeplitz completion in DOA estimation for nonuniform linear antenna arrays. II. Partially augmentable arrays

Abstract: This paper considers the problem of direction-of arrival (DOA) estimation for multiple uncorrelated plane waves incident on so-called "fully augmentable" sparse linear arrays. In situations where a decision is made on the number of existing signal sources (m) prior to the estimation stage, we investigate the conditions under which DOA estimation accuracy is effective (in the maximum-likelihood sense). In the case where m is less than the number of antenna sensors (M), a new approach called "MUSIC-maximum-entropy equalization" is proposed to improve DOA estimation performance in the "preasymptotic region" of finite sample size (N) and signal-to-noise ratio. A full-sized positive definite (p.d.) Toeplitz matrix is constructed from the M/spl times/M direct data covariance matrix, and then, alternating projections are applied to find a p.d. Toeplitz matrix with m-variate signal eigensubspace ("signal subspace truncations"). When m/spl ges/M, Cramer-Rao bound analysis suggests that the minimal useful sample size N is rather large, even for arbitrarily strong signals. It is demonstrated that the well-known direct augmentation approach (DAA) cannot approach the accuracy of the corresponding Cramer-Rao bound, even asymptotically (as N/spl rarr//spl infin/) and, therefore, needs to be improved. We present a new estimation method whereby signal subspace truncation of the DAA augmented matrix is used for initialization and is followed by a local maximum-likelihood optimization routine. The accuracy of this method is demonstrated to be asymptotically optimal for the various superior scenarios (m/spl ges/M) presented.

Habitat structure and regulation of local species diversity in a stony, upland stream

Abstract: Habitat structure may regulate species diversity at local scales, with complex habitats being normally associated with greater species richness than simple ones. We employed a new method of quantifying habitat structure to examine community structure in a stone-dwelling community of mobile macroinvertebrates inhabiting a perennial, upland stream (the Steavenson River) in southeastern Australia. We distinguished between the effects of increasing habitat structure by adding similar physical elements (habitat complexity) from that produced by adding qualitatively different sources of habitat structure (habitat heterogeneity) at spatial scales relevant to the biota. We used a field experiment to ask: (1) Does variation in habitat structure at local scales (i.e., between individual stones) result in variation in species richness (S) and numbers of individuals (N), and if so, are changes in S wrought passively by changes in N, or is there evidence of local regulation of S? (2) Are macroalgae, which are a source of habitat structure for invertebrates, also affected by stone surface structure? (3) What are the effects of habitat structure on faunal composition and body sizes? We used clay bricks as substrata and manipulated three sources of habitat structure in a crossed design: large surface pits and cracks (low density/high density); small pits caused by variation in surface texture (rough/smooth); and the abundance of macroalgae (begun with algae, begun without algae). The bricks were sampled for both fauna and epilithon on days 14 and 28 of colonization, when species richness and densities of individuals were comparable to natural stream stones. Habitat structure altered faunal diversity and abundances, with the majority of common species reaching higher abundances on creviced or rough surfaces. Rough surfaces were additionally associated with shifts in overall faunal composition and markedly smaller body sizes. Each element of habitat structure (large crevices, roughness, and macroalgae) promoted both increased species richness and densities of individuals. Rarefaction indicated that changes in S were disproportionate to changes in N, which suggests that S is regulated by local processes. Overall species richness was highest on day 14 with no difference in S between simple and complex surfaces. By day 28, simple surfaces had lost taxa relative to complex surfaces, suggesting that species richness in this stream community is regulated at a local scale, even though faunal composition changes continually and is contingent upon habitat structure. Habitat structure also affected the epilithon, suggesting that sources of habitat complexity and heterogeneity are interwoven in this system. Furthermore, the epilithon response to surface structure depended on the spatial scale of habitat complexity, with more of the red, filamentous alga Audouinella hermannii being found on rough surfaces than on smooth surfaces, but less on bricks with large crevices than without. These different responses to surface structure at different spatial scales demonstrate the importance of quantifying and manipulating substrate complexity at scales that are comparable with natural surfaces.

Evidence for a Role for AtMYB2 in the Induction of the Arabidopsis Alcohol Dehydrogenase Gene (ADH1) by Low Oxygen

Abstract: The transcription factor AtMYB2 binds to two sequence motifs in the promoter of the Arabidopsis ADH1 gene. The binding to the GT-motif (5'-TGGTTT-3') is essential for induction of ADH1 by low oxygen, while binding to the second motif, MBS-2, is not essential for induction. We show that AtMYB2 is induced by hypoxia with kinetics compatible with a role in the regulation of ADH1. Like ADH1, AtMYB2 has root-limited expression. When driven by a constitutive promoter, AtMYB2 is able to transactivate ADH1 expression in transient assays in both Arabidopsis and Nicotiana plumbaginifolia protoplasts, and in particle bombardment of Pisum sativum leaves. Mutation of the GT-motif abolished binding of AtMYB2 and caused loss of activity of the ADH1 promoter in both transient assays and transgenic Arabidopsis plants. These results are consistent with AtMYB2 being a key regulatory factor in the induction of the ADH1 promoter by low oxygen.

Aspects of geochronology and crustal evolution in the Eastern Fold Belt, Mt Isa Inlier∗

Abstract: Zircon U‐Pb SHRIMP ages and crustal Sm‐Nd signatures are reported for metasediments, meta‐volcanics, and a variety of pre‐ and post‐tectonic granitoid intrusives in the Eastern Fold Belt of the Mt Isa Inlier. These enable tracing of the broad crustal evolution of the rocks, erection of a chrono‐stratigraphic framework in the Eastern Fold Belt, and correlation of these rocks with those in other parts of the Mt Isa Inlier and in other Proterozoic inliers of northern Australia. The SHRIMP results and Sm‐Nd model ages provide quantitative information related to the provenance history of the metasediments and the sources and crustal interactions of magmatic rocks. Most of the latter have Nd Tdm of 2270–2200 Ma, suggesting similar source(s) to the metasediments and gneisses (2360–2140 Ma). The Nd TDM model ages are younger than those found in the Kalkadoon‐Leichhardt Belt, suggesting that the Eastern Fold Belt crust received less Archaean source input. Rocks of Barramundi age (1870–1840 Ma) are not found in the...

Motion estimation using a complex-valued wavelet transform

Abstract: This paper describes a new motion estimation algorithm that is potentially useful for both computer vision and video compression applications. It is hierarchical in structure, using a separable two-dimensional (2-D) discrete wavelet transform (DWT) on each frame to efficiently construct a multiresolution pyramid of subimages. The DWT is based on a complex-valued pair of four-tap FIR filters with Gabor-like characteristics. The resulting complex DWT (CDWT) effectively implements an analysis by an ensemble of Gabor-like filters with a variety of orientations and scales. The phase difference between the subband coefficients of each frame at a given subpel bears a predictable relation to a local translation in the region of the reference frame subtended by that subpel. That relation is used to estimate the displacement field at the coarsest scale of the multiresolution pyramid. Each estimate is accompanied by a directional confidence measure in the form of the parameters of a quadratic matching surface. The initial estimate field is progressively refined by a coarse-to fine strategy in which finer scale information is appropriately incorporated at each stage. The accuracy, efficiency, and robustness of the new algorithm are demonstrated in comparison testing against hierarchical implementations of intensity gradient-based and fractional-precision block matching motion estimators.

Ants as bioindicators of habitat disturbance: validation of the functional group model for Australia's humid tropics

Abstract: A functional group model of ant community composition has been widely used in Australia to analyse biogeographical patterns of ant community structure and the responses of ant communities to disturbance. The model has provided valuable support to the widespread use of ant communities as bioindicators of ecological change. However, the model was developed from studies of arid-zone faunas, and its applicability to the World Heritage rainforests of Queensland's humid tropics has not yet been validated. Here we test predictions based on the functional group model for ant communities in Queensland's humid rainforests, by documenting ant community composition and its responses to disturbance on the Atherton Tablelands. Five sites were studied, comprising two relatively undisturbed reference sites representing contrasting rainforest types, and three previously cleared sites, two of which were undergoing revegetation. A variety of sampling techniques were employed, including pitfall trapping, litter extractions, baiting, and general searching. A total of 50 ant species from 29 genera were collected. Site species richness was highest at the reference sites, and lowest at the unvegetated disturbed site, and overall was negatively related to mean ground temperature. As predicted by the functional group model, behaviorally dominant dolichoderines were uncommon or absent at the reference sites, and the most common ants were Generalized myrmicines and Opportunists. Also as predicted, habitat disturbance favored Opportunists, and, as the disturbance involved canopy clearance, this led to colonization by Iridomyrmex and other Dominant dolichoderines. Opportunists represented about 40% of total ants in traps at the reference sites, compared with 80–95% at the disturbed sites. Except one species, Tropical Climate Specialists and Specialist Predators were absent from disturbed sites. In conclusion, patterns of ant composition in relation to disturbance on the Atherton Tablelands conform to the functional group model that has been widely applied to ant faunas elsewhere in Australia. The model may therefore play an important role in the use of ants as bioindicators of ecological change in the World Heritage rainforests of this region.

Fractionation and Distribution of Arsenic in Soils Contaminated by Cattle Dip

Abstract: Soils surrounding cattle dips in Australia are known to be highly contaminated with As and are potentially of major concern to the environment and human health. A study of 11 dip sites in northern New South Wales (NSW) has revealed considerable surface soil (0-10 cm) contamination with As (37-3542 mg As kg -1 soil). In addition it was shown that considerable movement of As down through the soils had occurred with concentrations at 20 to 40 cm ranging from 57 to 2282 mg As kg -1 soil. At one particular site, an As concentration of above 14 000 mg As kg -1 soil was determined at a depth of 40 to 45 cm. A sequential fractionation scheme, based on a soil P fractionation, was developed to assess the chemical nature, and thus the potential bioavailability and mobility of As at the sites. Soil As is separated into six fractions with (i) anion exchange resin, (ii) NaHCO 3 , (iii) NaOH, (iv) NaOH following sonication, (v) HCl, and (vi) HCl/HNO 3 . Although substantial differences between sites and soil depths were present, some general trends were apparent. Most sites contained substantial concentrations of As in the two most labile fractions, indicating high potential for phytotoxicity and leaching. The bulk of the contaminant As at the sites seemed to be associated with soil amorphous Fe and Al minerals.

Transitions between Auhcoseira and Anabaena dominance in a turbid river weir pool

Abstract: The transitions between the diatoms Aulacoseira spp. (Melosira) and the cyanobacteria Anabaena spp. as dominant phytoplankton species in a turbid-river weir pool are shown to depend directly on the establishment or destruction of persistent thermal stratification. A transition from high to low flow through the pool resulted in the establishment of persistent thermal stratification, causing Aulacoseira to sink out of the euphotic zone at a speed of 0.95 m d-l. Concurrently, the slightly buoyant Anabaena grew within the euphotic zone with a specific growth rate of 0.37 d-l, climaxing after approximately 14 d at a population of 20,000-30,000 cells ml I, at which point its biomass may have been limited by the availability of phosphorus. The stratification thus caused the phytoplankton population to separate into two distinct layers, with Anabaena occupying the illuminated surface layer and Aulacoseiru found only in the lower layer below the euphotic depth. Under stratified conditions, the ratio of the surface layer depth to euphotic depth, z,, : z,,, was approximately 1, whereas for a mixed water column that ratio was >3. Access to light appeared to be the main factor determining the dominant phytoplankton species.

Analysis of starch structure using fluorophore‐assisted carbohydrate electrophoresis

Abstract: The analysis of the fine structure of starches is important to the investigation of linkages between starch structure and function and to the investigation of the properties and roles of starch biosynthetic, modifying and degradation enzymes. Fluorophore-assisted carbohydrate electrophoresis has recently been introduced as a method for the analysis of the oligosaccharide populations released by the enzymatic digestion of starches, which has advantages in resolution and sensitivity over previously used methods, and provides the capacity for the facile analysis of oligosaccharide populations on either a molar or mass basis. The use of fluorophore-assisted carbohydrate electrophoresis for the analysis of oligosaccharides is reviewed with particular reference to the choice of label, efficiency of labeling and separation techniques. Examples of separations using slab gel electrophoresis, DNA sequencer analysis and capillary electrophoresis are presented and we conclude that on the basis of resolution and reproducibility, capillary electrophoresis is the method of choice for the separation of oligosaccharides of degree of polymerization from 1 to 100. Examples of isoamylase-debranched starches and glycogens analyzed by capillary electrophoresis are presented. The capillary electrophoresis analysis of starch structure through the analysis of oligosaccharides released by the debranching of limit dextrins derived from starches and glycogens is introduced as a useful diagnostic of starch structure. The potential for future development of novel diagnostics for starch structure using fluorophore-assisted carbohydrate electrophoresis is discussed.

Late Quaternary sediment facies in Prydz Bay, East Antarctica and their relationship to glacial advance onto the continental shelf

Abstract: A marine survey in Prydz Bay, provides an unparalleled view of glacigenic and marine sedimentation across Prydz Channel and Amery Depression during the Late Quaternary. Gravity cores and a suite of eight radiocarbon dates indicate that the Late Wisconsin Glacial Maximum (LGM) was associated with grounding of a palaeo-ice shelf along the periphery of Prydz Channel. Deposition in front of the grounding line was dominated by ice-rafting. A granulated facies, containing angular clay and diamicton clasts, was producd by a combination of regelation freezing, near to the grounding line, and remelting of this basal debris in the sub-ice shelf setting. Beneath these LGM marine deposits lie two key beds of diatom ooze that are distinct in size sorting and Pliocene diatoms. These “interstadial” units can be traced across most of the Prydz Channel, and are underlain by additional glacial marine units. Debris related to the Lambert Deep is distinct from detritus from eastern Prydz Bay and deposition of these two sources within the channel oscillated during the LGM. We suggest that coastal drainage systems contributed to a limited glaciation of the shelf during the LGM, rather than direct outflow via the Lambert/Amery system. It is proposed that shelf-wide glaciation is related to the duration of glacial sea level lowstands rather than the absolute magnitude of eustatic fall during such episodes.