Deen Dayal Upadhyay Gorakhpur University

About: Deen Dayal Upadhyay Gorakhpur University is a education organization based out in Gorakhpur, Uttar Pradesh, India. It is known for research contribution in the topics: Thermal decomposition & Lymnaea acuminata. The organization has 1032 authors who have published 1591 publications receiving 21734 citations. The organization is also known as: Gorakhpur University.

Effect of the synthetic pyrethroid Deltamethrin and the neem-based pesticide Achook on the reproductive ability of zebrafish, Danio rerio (Cyprinidae)

Abstract: In the present research, both male and female zebrafish, Danio rerio (Hamilton), were exposed to 96-h LC5 values of Deltamethrin (0.016 µg dm -3 ) and Achook (0.025 µg dm -3 ) for three months. The fish were then returned to normal water and allowed to breed to permit observations of fecundity and hatchability. The results show significant reductions in fecundity and hatchability in comparison to the control group. Fecundity was reduced by 54.12% in the fish treated with Deltamethrin and by 17.81% in those treated with Achook. Significant decreases in hatchability of up to 49.7% were noted in the Deltamethrin group and of 36.9% in the Achook treated fish. The number of unhatched/dead eggs increased significantly (P < 0.05 for each case). It was concluded that low concentrations (96-h LC5 values) of both pesticides can have a significant impact on the reproduction of zebrafish.

Antifungal principle ofRanunculus sceleratus

Abstract: So far antibiotic substances have largely been obtained from microorganisms, most of which act as antibacterials and do not show antifungal activity. Search for antibiotics from higher plants remained neglected for a long time but during recent years a large number of plants have been found to contain antibacterial substances. Antifungal substances from higher plants are comparatively little known, but the search for them continues on an increasing scale. Several plants have been screened for their fungitoxicity (Gilliver, 1947; Basu and Bose, 1956; Abdullaeva, 1959; Petrushova and Meshechaninova, 1961; Nene and Thapliyal, 1965; Thapliyal and Nene, 1967; Dhar et al., 1968; Gupta and Banejee, 1970; Nicolls, 1970; Shekhawat and Prasad, 1971) but attempts to isolate and identify their active principle(s) are mostly wanting. The present paper records the isolation, identification, fungitoxicity, phytotoxicity and systemic activity of the antifungal active principle of Ranunculus sceleratus L. (Hindi: jaldhania; English: buttercup or water crowfoot), a widely occurring winter season herb with small, pale yellow flowers and nectaries at the base of the petals. It is also cultivated in gardens for its beautiful flowers and has been reported for its medicinal properties (Trivedi, 1965). Earlier, leaf extracts were found to possess strong fungicidal activity against several test fungi by the present authors (Misra et al., 1974; Misra, 1975; Misra and Dixit, 1976, 1977).

PURIFICATION AND CHARACTERIZATION OF A THERMOSTABLE SOLUBLE PEROXIDASE FROM Citrus medica LEAF

Abstract: A soluble and thermostable peroxidase enzyme (POD) was extracted from the leaf of Citrus medica. The enzyme was purified 15.10-fold with a total yield of 28.6% by ammonium sulfate precipitation followed by Sephadex G-100 gel filtration chromatography. The purified enzyme came as a single band on native polyacrylamide gel electrophoresis (PAGE) as well as sodium dodecyl sulfate (SDS) PAGE. The molecular mass of the enzyme was about 32 kD as determined by SDS-PAGE. The enzyme was optimally active at pH 6.0 and 50°C temperature. The enzyme was active in wide range of pH (5.0–8.0) and temperature (30–80°C). From the thermal inactivation studies in the range of 60–75°C, the half-life (t1/2) values of the enzyme ranged from 8 to 173 min. The inactivation energy (Ea) value of POD was estimated to be 21.7 kcal mol−1. The Km values for guaiacol and H2O2 were 8 mM and 1.8 mM, respectively. This enzyme was activated by some metals and reagents such as Ca2+, Cu2+, Mg2+, Co2+, ferulic acid, and indole acetic acid (IAA...

A Review on Chalcones Synthesis and their Biological Activity

Abstract: Chalcones are a valuable molecule of medicinal importance due to presence of reactive ketoethylenic group –CO–CH=CH–, belonging to the flavonoid family These reactive α,β-unsatutated keto function in chalcones are responsible for their biological activity Chalcone can be synthesized by several methods using aldehydes and ketones as starting material This review is focused about different methods of synthesis and versatile biological activity of chalcones including antimicrobial, anticancer, antioxidant, antimalarial, antituberculosis etc