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Institution

Deen Dayal Upadhyay Gorakhpur University

EducationGorakhpur, Uttar Pradesh, India
About: Deen Dayal Upadhyay Gorakhpur University is a education organization based out in Gorakhpur, Uttar Pradesh, India. It is known for research contribution in the topics: Thermal decomposition & Lymnaea acuminata. The organization has 1032 authors who have published 1591 publications receiving 21734 citations. The organization is also known as: Gorakhpur University.


Papers
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Journal ArticleDOI
TL;DR: The extracellular α-l-rhamnosidase has been purified by growing a new fungal strain Aspergillus awamori MTCC-2879 in the liquid culture growth medium containing orange peel, thereby contributing to the preparation of pharmaceutically important compounds like prunin and l- rhamnose.
Abstract: Summary The extracellular α-l-rhamnosidase has been purified by growing a new fungal strain Aspergillus awamori MTCC-2879 in the liquid culture growth medium containing orange peel. The purification procedure involved ultrafiltration using PM-10 membrane and anion-exchange chromatography on diethyl amino ethyl cellulose. The purified enzyme gave single protein band in SDS-PAGE analysis corresponding to molecular mass 75.0 kDa. The native PAGE analysis of the purified enzyme also gave a single protein band, confirming the purity of the enzyme. The Km and Vmax values of the enzyme for p-nitrophenyl-α-l-rhamnopyranoside were 0.62 mm and 27.06 μmole min−1 mg−1, respectively, yielding kcat and kcat/km values 39.90 s−1 and 54.70 mm−1 s−1, respectively. The enzyme had an optimum pH of 7.0 and optimum temperature of 60 °C. The activation energy for the thermal denaturation of the enzyme was 35.65 kJ−1 mol−1 K−1. The purified enzyme can be used for specifically cleaving terminal α-l-rhamnose from the natural glycosides, thereby contributing to the preparation of pharmaceutically important compounds like prunin and l-rhamnose.

17 citations

Journal ArticleDOI
TL;DR: In this article, the reactions of bis(cyclopentadienyl)titanium(IV) chloride with a new series of Schiff bases (LH2), derived by condensing 3-(phenyl/2-chlorophenyl/4-nitrophenyl)-4-amino-5-hydrazino-1,2,4-triazoles with salicylaldehyde or 2-hydroxyacetophenone, have been studied both by conventional stirring method and also by using microwave technology.
Abstract: The reactions of bis(cyclopentadienyl)titanium(IV) chloride with a new series of Schiff bases (LH2), derived by condensing 3-(phenyl/2-chlorophenyl/4-nitrophenyl)-4-amino-5-hydrazino-1,2,4-triazoles with salicylaldehyde or 2-hydroxyacetophenone, have been studied both by conventional stirring method and also by using microwave technology. The products of type [(η5-C5H5)2Ti(L)] have been isolated in both cases. Tentative structural conclusions are drawn for the reaction products based upon physico-chemical and spectroscopic methods. The ligands behave as dibasic, tetradentate chelating agents and a six-coordinated structure have been assigned to these derivatives. Studies were conducted to assess the growth inhibiting potential of the free Schiff bases and their complexes against various bacterial strains.

17 citations

Journal ArticleDOI
TL;DR: Garlic holds a unique therapeutic potential as it inhibits invasion of carcinoma, provides cardiovascular protection, lowering of cholesterol, blood pressure, anti-platelet activities, and thromboxane formation, and its constituents could be used to develop alternatives to conventional insecticides for control of serious fruit and vegetable pests.
Abstract: Garlic (Allium sativum) holds a unique therapeutic potential as it inhibits invasion of carcinoma, provides cardiovascular protection, lowering of cholesterol, blood pressure, anti-platelet activities, and thromboxane formation. It provides protection against atherosclerosis and associated disorders and helps to decrease serum levels of glucose, insulin, triglycerides, and uric acid, as well as insulin resistance, and reduces cytokine levels. It shows hypolipidemic, anti-platelet, and procirculatory effects, and antimutagenic and antiproliferative properties. It prevents cold and flu symptoms through immune enhancement and exhibits anticancer and chemopreventive activities. The main active component of garlic is alliin (S-allyl cysteine sulfoxide), a potent antioxidant which shows cardioprotective and neuroprotective actions. Diallyl trisulfide, major garlic derivatives, could inhibit the cell proliferation by triggering either cell cycle arrest or apoptosis in a variety of cancer cells. Organosulfur compounds from garlic inhibit the growth of transplanted as well as spontaneous cancers in preclinical animal models without any adverse side effects. Garlic is a good source of anti-invasive, antioxidant, anti-inflammatory, immunomodulatory, chemopreventive, hepatoprotective, antimicrobial, apoptotic, cardioprotective, antidiabetic agents and shows insecticidal effects against lepidopteran, coleopteran, dipteran and homopteran insect pests. Hence, its constituents could be used to develop alternatives to conventional insecticides for control of serious fruit and vegetable pests. Garlic herbal preparations can reduce non-target exposure to hazardous insecticides and curb resistance development in insects. No doubt garlic based different ailments and concoctions can be used to alleviate a variety of health problems. Its various supplements contain a different concentration of organosulfur compounds are available commercially in market

17 citations

Journal ArticleDOI
TL;DR: The non-ideal behaviour of the systems studied is explained on the basis of dipole-induced dipole interactions.

17 citations

Journal ArticleDOI
TL;DR: The biophysical studies on pectin lyase showed that its secondary structure belongs to α + β class of protein with comparatively less of β‐sheets, and it showed efficient retting of Crotolaria juncea fibers.
Abstract: An extracellular pectin lyase secreted by Fusarium decemcellulare MTCC 2079 under solid state fermentation condition has been purified to electrophoretic homogeniety by using ammonium sulfate fractionation, carboxymethyl cellulose and gel filtration (Sephadex G-100) column chromatographies. The purified enzyme showed single protein band corresponding to molecular mass 45 ± 01 kDa on sodium dodecyl sulfate polyacrylamide gel electrophoresis. The enzyme had maximum activity at pH 9.0 and showed maximum stability in the pH range of 9.0-12.0. The optimum temperature of the purified enzyme was 50 °C and it showed maximum stability upto 40 °C. The energy of activation for the thermal denaturation (Ea ) was 59.06 kJ mol(-1) K(-1). The Km and kcat values using citrus pectin as the substrate were 0.125 mg ml(-1) and 72.9 s(-1) in 100 mM sodium carbonate buffer pH 9.0 at 50 °C. The biophysical studies on pectin lyase showed that its secondary structure belongs to α + β class of protein with comparatively less of β-sheets. Purified pectin lyase showed efficient retting of Crotolaria juncea fibers.

17 citations


Authors

Showing all 1045 results

NameH-indexPapersCitations
Rudra Deo Tripathi571389640
Nawal Kishore Dubey5022910796
Harikesh Bahadur Singh463077372
Souvik Maiti432375759
Ajay Singh392568464
Alok C. Gupta391314052
Suman K Mishra382404989
Gurdip Singh361575173
Ram C. Mehrotra355066259
Nidhi Gupta352664786
Ajay K. Mishra342195050
Seema Mishra33794312
Narsingh Bahadur Singh331944062
Manish Naja321103383
Maya Shankar Singh312454261
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Performance
Metrics
No. of papers from the Institution in previous years
YearPapers
20239
202216
2021118
202094
201965
201869