Department of Biotechnology

About: Department of Biotechnology is a government organization based out in New Delhi, India. It is known for research contribution in the topics: Population & Silver nanoparticle. The organization has 4800 authors who have published 5033 publications receiving 82022 citations.

Analysis of the opportunities and challenges of solar water heating system (SWHS) in India: Estimates from the energy audit surveys & review

Abstract: A solar water heating system (SWHS) is a device that makes available the thermal energy of the incident solar radiation for use in various water heating applications. SWHS largely depends on the performance of the collector's efficiency at capturing the incident solar radiation and transferring it to the water. With today's SWHS, water can be heated up to temperatures of 60–80 °C. Heated water is collected in a tank insulated to prevent heat loss. Circulation of water from the tank through the collectors and back to the tank continues automatically due to the thermosiphon principle. The hot water generated finds many end-use applications in domestic, commercial, and industrial sectors. India has the highest energy intensities in Asia. Very little investment and priority are being given to increase of the efficiency. On the other hand, the India has a high potential for developing energy production from renewable energy sources (RES): solar, water, wind and biomass. However, these potentials are not studied and exploited enough and the present situation for their utilization is not so good. Although energy is a critical foundation for economic growth and social progress of any country, there are many constraints for RES development in all of them (political, technological, financial, legislative, educational, etc.). Obviously, defining development strategies and new support measures is necessary since renewable energy sources can make an important contribution to the regional energy supply and security. The main purpose of this paper is to explore the solar water heating system (opportunities) in India.

Purification and characterization of alkaline chitinase from Paenibacillus pasadenensis NCIM 5434

Abstract: An alkaline chitinase was purified from the bacterium Paenibacillus pasadenensis NCIM 5434 isolated from alkaline littoral soil of Lonar Lake. The chitinase was purified by ammonium sulfate precipitation followed by DEAE cellulose column chromatography. Enzyme was purified by 8.87 folds with 24.96% yield. Molecular characterization through SDS–PAGE analysis showed that it has molecular weight of about ∼35 kDa. The enzyme kinetics studies of purified chitinase revealed the following characteristics, Km 6.25 mg ml−1 and Vmax 434.78 µM for colloidal chitin as a substrate. The chitinase showed optimum pH 10 and temperature 37 °C. The enzyme exhibited significant activity up to 3% salt concentration, indicating saline nature. Its activity was enhanced with calcium, potassium and magnesium; whereas copper and mercury were found to be inhibitory. Since, it showed antifungal activity against Penicillium and Aspergillus, it could be used as powerful biocontrol agent.

Biodegradation of beta-cyfluthrin by Pseudomonas stutzeri strain S1

Abstract: β-Cyfluthrin [α-cyano-4-fluoro-3-phenoxybenzyl-3(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylate] pesticide has been in agricultural use in the recent years for controlling Lepidopteran pests affecting solanaceous crops. The extensive use of synthetic pyrethroids like β-cyfluthrin has resulted in wide spread environmental contamination. The purpose of this study was to isolate bacteria from soil and to determine their ability to degrade β-cyfluthrin and identify the intermediates in culture broth using spectroscopy. An aerobic bacterium capable of degrading β-cyfluthrin was isolated by enrichment culture. The 16S ribosomal DNA sequence of the isolate (strain S1) had 100% identity to the sequence from Pseudomonas stutzeri. Finally products formed during degradation of β-cyfluthrin have been identified as α-cyano-4-fluoro-3-phenoxybenzyl-3(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylate (M.W. 341); 4-fluoro-3-phenoxy-α-cyanobenzyl alcohol (M.W. 243) and 3(2,2-dichlorovinyl)-2,2-dimethyl cyclopropanecarboxylic acid (M.W. 208).

Biology and survival of extremely halophilic archaeon Haloarcula marismortui RR12 isolated from Mumbai salterns, India in response to salinity stress.

Abstract: Haloarchaea are unique microorganism's resistant to environmental and osmotic stresses and thrive in their habitats despite extreme fluctuating salinities In the present study, haloarchaea were isolated from hypersaline thalossohaline salterns of Bhandup, Mumbai, India and were identified as Haloferax prahovense, Haloferax alexandrines, Haloferax lucentense, Haloarcula tradensis, Haloarcula marismortui and Haloarcula argentinensis The mechanism of adaptation to contrasting salinities (15 M and 45 M) was investigated in the extreme haloarchaeon, Hal marismortui RR12 Hal marismortui RR12 increased the intracellular sequestration of K(+) and Cl(-) ions in hypo salinity and hyper salinity respectively as detected by Energy-dispersive X-ray spectroscopy microanalysis (EDAX) and Inductively Coupled Plasma- atomic Emission Spectroscopy (ICP-AES) indicating the presence of 'salt-in' strategy of osmoadaptation As a cellular response to salinity stress, it produced small heat shock like proteins (sHSP) identified using MALDI-TOF MS and increased the production of protective red carotenoid pigment This is the first report on the study of the concomitant cellular, molecular and physiological mechanism adapted by Hal marismortui RR12 when exposed to contrasting salinities in external environment