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Institution

Department of Biotechnology

GovernmentNew Delhi, India
About: Department of Biotechnology is a government organization based out in New Delhi, India. It is known for research contribution in the topics: Population & Silver nanoparticle. The organization has 4800 authors who have published 5033 publications receiving 82022 citations.


Papers
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Journal ArticleDOI
TL;DR: The first report on gene expression dynamics in thrips infested Darjeeling tea leaves can be extrapolated with increase in volatiles which is responsible for enhancing the quality of Darjeels tea, specially the flavour and aroma of the infusion.
Abstract: Darjeeling teas are the highest grown teas in the world and preferred for its flavour, aroma and quality. Apart from the genetic makeup of the plant, earlier reports suggest that insect infestation, particularly jassids and thrips triggers the aroma and flavour formation in Darjeeling tea. The present work encompasses the identification of the genes/transcriptomes responsible for the typical flavour of Darjeeling tea, besides understanding the role of jassids and thrips in particular, in producing the best cup character and quality. The quantitative real time PCR analysis was based on a suppression subtractive hybridisation forward library of B157 (tea clone infested with thrips), providing us transcripts related to aroma and flavour formation. We observed the expression of genes like leucine zipper, ntd, nced, geraniolsynthase, raffinose synthase, trehalose synthase, amylase, farnesyl transferase, catalase, methyl transferase, linalool synthase, peroxidases, elicitor responsive proteins, linamarase, nerolidol linalool synthase 2, 12-oxophytodienoate reductase, glucosidase, MYB transcription factor, and alcohol dehydrogenase, highly regulated due to insect infestation, manufacturing stresses and mechanical injury. The first report on gene expression dynamics in thrips infested Darjeeling tea leaves can be extrapolated with increase in volatiles which is responsible for enhancing the quality of Darjeeling tea, specially the flavour and aroma of the infusion. We hope to model these responses in order to understand the molecular changes that occur during Darjeeling tea flavour formation.

46 citations

Journal ArticleDOI
01 Mar 2014
TL;DR: The present study suggests that the use of single strain inoculum of CKT1 with multiple plant growth promoting activities offers a recent concept to address multiple mode of action by combined use of multi-strain inocula of PGPR with each having specific capability and function.
Abstract: The use of plant growth promoting rhizobacteria (PGPR) is steadily increasing in agriculture and offers an attractive way to replace chemical fertilizers, pesticides, and supplements. The authors have isolated and characterized different PGPRs from the rhizosphere soils/roots of tomato with multiple plant growth promoting activities. Subsequently, to investigate the effects of PGPR isolates on the growth of tomato, a pot culture experiment was conducted under net house. Most of the isolates resulted in a significant increase in shoot length, root length and dry matter production of shoot and root of tomato seedlings. Among eleven isolates, N11 exhibited concomitant production of all plant growth promoting activities viz., phosphate solubilization, siderophore production, indole-acetic acid production including hydrogen cyanide production. Remarkable increase was observed in seed germination (36.08 %), shoot length (5.22 %), root length (21.12 %), shoot dry weight (63.50 %) and root dry weight (54.08 %), nitrogen (18.75 %), potassium (57.69 %) and phosphorus (22.22 %). Morphological, biochemical and 16S rRNA gene analysis identified strain N11 as Bacillus subtilis strain CKT1. The present study, therefore, suggests that the use of single strain inoculum of CKT1 with multiple plant growth promoting activities offers a recent concept to address multiple mode of action by combined use of multi-strain inoculum of PGPR with each having specific capability and function.

46 citations

Journal ArticleDOI
TL;DR: In this paper, the use of magnetic nanomaterials to adsorb dyes from aqueous solutions is discussed, as well as the impact of dyes on health and the environment, dye removal methodologies, and the advantages of magnetic separation.
Abstract: Dye pollution from industries such as dyeing, textile, leather, cosmetics and pigments is of increasing concern, calling for advanced methods of wastewater remediation. Many techniques have been applied to treat such effluents, but adsorption remains the easiest, low-cost, effective and reliable strategies. The recent development of magnetic composites combines properties of both organic and inorganic components and is thus opening new applications for color removal. Here, we review the use of magnetic nanomaterials to adsorb dyes from aqueous solutions. We present sources of water contamination, actual technologies for wastewater treatment, the impact of dyes on health and the environment, dye removal methodologies, and the advantages of magnetic separation. We also discuss nanoparticle synthesis, results on magnetic nanomaterials for dye adsorption, and adsorption models.

46 citations

Journal ArticleDOI
TL;DR: The catalytic activity of synthesised AgNPs was assessed against azo dyes such a Methylene Blue and Congo Red that resulted in its effective degradation of toxic compounds in a short span of time.
Abstract: The present study focuses on the catalytic, antibacterial and antibiofilm efficacy of silver nanoparticles (AgNPs) in an easy, rapid and eco-friendly pathway. Herein, we have synthesised AgNPs using an aqueous extract of P. juliflora leaf. The bioactive compounds present in the extract are responsible for the reduction of Ag+ to Ag0. The particle synthesis was first observed by visual color change and then characterized using UV–visible spectroscopy to confirm the formation of AgNPs. The synthesis conditions were then optimised using critical parameters such as reaction time, AgNO3 concentration, extract to AgNO3 ratio and temperature of the reaction. The hydrodynamic size of the AgNPs with Dynamic light scattering (DLS) was 55.24 nm, while, was in the range of 10–20 nm as determined through Transmission Electron Microscopy (TEM). Further, Fourier transform infrared spectroscopy (FTIR) studies were conducted to discern the functional groups or compounds responsible for the reduction of silver nitrate as well as the capping of silver nanoparticles. Later, X-ray diffraction (XRD) results showed crystalline nature of the biosynthesized AgNPs. To evaluate their antibacterial potential, AgNPs were assessed through disc-diffusion assay, which resulted in an appreciable dose-dependent activity. The antibacterial potential was investigated through disc-diffusion assay against E. coli and P. aeruginosa. The Congo red agar (CRA) plate assay successfully revealed the anti-biofilm activity against B. subtilis and P. aeruginosa. Further, the catalytic activity of synthesised AgNPs was assessed against azo dyes such a Methylene Blue (MB) and Congo Red (CR) that resulted in its effective degradation of toxic compounds in a short span of time. Further, AgNPs were assessed for their wound healing potential.

46 citations

Journal ArticleDOI
01 Apr 2019
TL;DR: In this paper, the gold nanoparticles (GA-AuNPs) produced from Gelidiella acerosa, marine algae using green synthesis method was evaluated for its antidiabetic, antibacterial and antioxidant activity.
Abstract: In this present research, the Gold nanoparticles (GA-AuNPs) produced from Gelidiella acerosa, marine algae using green synthesis method was evaluated for its antidiabetic, antibacterial and antioxidant activity. Well-developed GA-AuNPs were effectively characterized using advanced analytical instruments like HRTEM, UV–visible, SEM and XRD. This analysis exposed GA-AuNPs to be present with spherical, hexagonal structure and crystalline nature. The average size of GA-AuNPs particles was estimated to lie between 5.81 nm to 117.59 nm by using Debye–Scherer equation. The enzyme inhibitory property of (IC50) of G. acerosa aqueous extract and synthesized gold nanoparticles from G. acerosa against α amylase and α- glucosidase enzyme was found to be 2.1 ± 0.01, 3.7 ± 0.01 µg/mL and 2.8 ± 0.02, 4.1 ± 0.01, respectively. Further antioxidant assessment of GA-AuNPs were examined using DPPH and FRAP assays, the results exhibited that the strong antioxidant activity were observed in all the concentrations when compared with standard. Finally antibacterial activity was evaluated the GA-AuNPs against gram positive and gram negative bacteria, the strong antibacterial activity was observed in both the bacterial strains when compared with standard antibiotic. The GA-AuNPs from marine algae was exhibited to be strong inhibitory activity against alpha amylase and alpha glucosidase enzyme, antioxidant activity and antibacterial activity, these GA-AuNPs are projected to have potential biological applications.

46 citations


Authors

Showing all 4812 results

NameH-indexPapersCitations
Ashok Pandey9679643038
Klaus Becker7932027494
Bansi D. Malhotra7537519419
Ashwani Kumar6670318099
Sanjay K. Banerjee6279830044
M. Michael Gromiha5635210617
Swaran J.S. Flora5526711434
Mallappa Kumara Swamy5486414508
Pulok K. Mukherjee5429610873
Mukesh Doble513649826
Jaya Narayan Sahu491579569
Pradeep Das4942610118
Jon R. Lorsch481177661
Rakesh Tuli471657497
Amit K. Goyal471575749
Performance
Metrics
No. of papers from the Institution in previous years
YearPapers
20231
202261
2021948
2020648
2019572
2018427