Department of Biotechnology

About: Department of Biotechnology is a government organization based out in New Delhi, India. It is known for research contribution in the topics: Population & Silver nanoparticle. The organization has 4800 authors who have published 5033 publications receiving 82022 citations.

Synthesis and Characterization of Chitosan/Silver Nanocomposite Using Rutin for Antibacterial, Antioxidant and Photocatalytic Applications

Abstract: The present study reports an eco-friendly synthesis of chitosan/silver (CS/Ag) nanocomposite using rutin by a bio-inspired method. The formation of brown color and UV–visible absorbance peak at 415 nm confirmed the synthesis of CS/Ag nanocomposite. X-ray diffraction (XRD) analysis revealed the crystalline peaks of both chitosan and silver in synthesized CS/Ag nanocomposite. Field emission-scanning electron microscopy (FE-SEM) analysis showed that the synthesized CS/Ag nanocomposite having predominant spherical shaped nanostructure with an average size of 23–78 nm. Fourier transform infrared spectroscopy (FTIR) analysis indicated the presence of functional derivatives related to chitosan and rutin in CS/Ag nanocomposite. Synthesized CS/Ag nanocomposite exhibited superior disc diffusion antibacterial activity against Bacillus subtilis and Escherichia coli. The in vitro antioxidant of CS/Ag nanocomposite was evaluated spectrophotometrically by using DPPH method. The percentage of antioxidant activity was increased with increasing concentration of CS/Ag nanocomposite. Furthermore, photocatalytic activity of CS/Ag nanocomposite was evaluated by the removal of methylene blue (MB) dye from aqueous solution under sunlight irradiation. Results showed that the degradation efficiency of MB reached up to 88% after irradiation for 220 min. Thus, the obtained results indicated that the synthesized CS/Ag nanocomposite using rutin can be a promising nanomaterial for multifunctional applications.

Screening of potential lead molecules against prioritised targets of multi-drug-resistant-Acinetobacter baumannii – insights from molecular docking, molecular dynamic simulations and in vitro assays

Abstract: Acinetobacter baumannii, an opportunistic pathogen, has become multi-drug resistant (MDR) to major classes of antibacterial and poses grave threat to public health. The current study focused to scr...

Proximate composition and sensory evaluation of anthocyanin-rich purple sweet potato (Ipomoea batatas L.) wine

Abstract: Purple sweet potato (PSP) is a special type of sweet potato having high anthocyanin pigment in the root. The starch contents of PSP (root/water homogenised in 1:1 ratio) were enzymatically saccharified [using commercial thermostable enzymes Termamyl® (0.2%) and Dextrozyme®GA (1%)] to fermentable sugars, and the filtrate was ameliorated with cane sugar to achieve 20° Brix, for subsequent fermentation into a red wine using 2% yeast (Saccharomyces cerevisiae) as starter culture. The wine had the following proximate compositions: total soluble sugar (TSS), 2.25° Brix; starch, 0.15 g per 100 mL; total sugar, 1.35 g per 100 mL; TA, 1.34 g tartaric acid per 100 mL; phenol, 0.36 g (caffeic acid equivalent) per 100 mL; anthocyanin, 55.09 mg per 100 mL; tannin, 0.64 mg per 100 mL; lactic acid, 1.14 mg per 100 mL; ethanol, 9.33%(v/v); and pH, 3.61. 2, 2‐Diphenyl‐1‐picrylhydrazyl (DPPH) scavenging activity of the wine was 58.95% at a dose of 250 I¼g mL−1. Principal component analysis reduced the eleven original analytical and proximate variables (TSS, starch, total sugar, TA, phenol, anthocyanin, tannin, lactic acid, ethanol, pH, DPPH) to four independent components, which accounted for 76.65% variations. Sensory evaluation was carried out by sixteen trained panellists on various attributes such as clarity, colour, viscosity, odour, flavour, spritz and finish. Independent ‘t’ test confirmed that all the sensory attributes of the PSP wine scored closely (within 10% variation) to that of a commercial grape wine. The red wine produced contains essential antioxidants and acceptable sensory qualities.