Department of Biotechnology

About: Department of Biotechnology is a government organization based out in New Delhi, India. It is known for research contribution in the topics: Population & Silver nanoparticle. The organization has 4800 authors who have published 5033 publications receiving 82022 citations.

Eukaryotic translation initiation factor eIF5 promotes the accuracy of start codon recognition by regulating Pi release and conformational transitions of the preinitiation complex

Abstract: eIF5 is the GTPase activating protein (GAP) for the eIF2 · GTP · Met-tRNAi (Met) ternary complex with a critical role in initiation codon selection. Previous work suggested that the eIF5 mutation G31R/SUI5 elevates initiation at UUG codons by increasing GAP function. Subsequent work implicated eIF5 in rearrangement of the preinitiation complex (PIC) from an open, scanning conformation to a closed state at AUG codons, from which Pi is released from eIF2 · GDP · Pi. To identify eIF5 functions crucial for accurate initiation, we investigated the consequences of G31R on GTP hydrolysis and Pi release, and the effects of intragenic G31R suppressors on these reactions, and on the partitioning of PICs between open and closed states. eIF5-G31R altered regulation of Pi release, accelerating it at UUG while decreasing it at AUG codons, consistent with its ability to stabilize the closed complex at UUG. Suppressor G62S mitigates both defects of G31R, accounting for its efficient suppression of UUG initiation in G31R,G62S cells; however suppressor M18V impairs GTP hydrolysis with little effect on PIC conformation. The strong defect in GTP hydrolysis conferred by M18V likely explains its broad suppression of Sui(-) mutations in numerous factors. We conclude that both of eIF5's functions, regulating Pi release and stabilizing the closed PIC conformation, contribute to stringent AUG selection in vivo.

Immunomodulatory activity of geranial, geranial acetate, gingerol, and eugenol essential oils: evidence for humoral and cell-mediated responses.

Abstract: Objective: The immunomodulatory effect of geranial, geranial acetate, gingerol, and eugenol essential oils were evaluated by studying humoral and cell-mediated immune responses Materials and Method: The essential oils were evaluated for immunomodulatory activity in in vivo studies, using rats as the animal model The essential oils were tested for hypersensitivity and hemagglutination reactions, using sheep red blood cells (SRBC) as the antigen while sodium carboxy methyl cellulose (SCMC) served as the control in all the tests Result: Orally administrated essential oils showed a significant increase of test parameters, viz, haemagglutinating antibody titre (HAT) and delayed type hypersensitivity (DTH) response In rats immunized with sheep RBC, essential oils enhanced the humoral antibody response to the antigen and significantly potentiated the cellular immunity by facilitating the foot pad thickness response to sheep RBC in sensitized rats with doses of 50-800 mg/ml Haemagglutination titre of geraniol showed the highest increase of 1393±638 and with 59±07 DTH, respectively For geranial acetate, the haemagglutination titre showed a moderate increase of 875±59 and highest increase in DTH with 59±08, respectively Using gingerol, the haemagglutination titre showed a moderate increase with 882±6306 and DTH 35±05, respectively and for eugenol, the haemaggulation titre showed a moderate increase with 11206±6169 and DTH 44±06, respectively These differences were statistically significant Conclusion: The essential oils were found to have a significant immunostimulant activity on both the specific and non-specific immune mechanisms

Effect of biofertilizer, vermicompost and chemical fertilizer on different biochemical parameters of Glycine max and Vigna mungo

Abstract: The objective of any agricultural research program is to increase the level of crop productivity. The strategy to boost the level of crop productivity would be through the adoptionof package of practices comprising use of seeds of high yielding varieties, adequate doses of manures and fertilizers and plant protection chemicals. Seed germination is one of the important factors for progressive farming. In present investigation, the effect of plant fertilizers on growth and productivity of Glycine max and Vigna mungo were studied at the various concentrations of fertilizers. We used Biofertilizer (B), Vermicompost (V), Chemical fertilizer (C) and combination of these three. It was found that,the seed germination percentage was high in Vermicompost treated soil in Vigna mungo and Biofertilizer treated in Glycine max. Vigna mungo protein content was found high in B+C treated soil, while carbohydrate and phenol content increased in B+V treated soil. High seed germination was observed in vermicompost treated soil .In case of Glycine max, carbohydrate content increased in C+V+B treated soil ,protein and phenol content observed max. in vermicompost treated soil and high seed germination % observed in biofertilizer treated soil.