Department of Biotechnology

About: Department of Biotechnology is a government organization based out in New Delhi, India. It is known for research contribution in the topics: Population & Silver nanoparticle. The organization has 4800 authors who have published 5033 publications receiving 82022 citations.

Green synthesis of silver nanoparticles using leaf extract of Holoptelea integrifolia and preliminary investigation of its antioxidant, anti-inflammatory, antidiabetic and antibacterial activities

Abstract: In present study, silver nanoparticles (AgNPs) were synthesized from the aqueous extract of Holoptelea integrifolia (HI) leaves and characterized by UV–vis spectroscopy, Fourier transformation infrared spectroscopy (FTIR), field emission scan electron microscopy (FESEM), electron disperse X-ray (EDX) and X-ray diffraction (XRD) analysis. UV–vis study confirmed the formation of AgNPs. FTIR studies confirmed the presence of OH and NH functional groups of secondary molecules of HI capped on the AgNPs surface. FESEM studies revealed the formation of aggregates of spherical shape of size 32–38 nm. Presence of elemental silver was confirmed by EDX studies. Face centred cubic (FCC) crystal structure of biosynthesized AgNPs was governed by the XRD analysis. The biosynthesized AgNPs exhibited significant antioxidant activities (51.49 ± 3.33, 41.18 ± 2.27, and 74.59 ± 3.08% for the DPPH, metal chelating, and nitric oxide assay). Remarkable anti-diabetic (86.66 ± 5.03%), anti-inflammatory (binding constant 2.60 ± 0.05 × 10−4) and antibacterial (MIC from 75 to 150 μl) activities were noticed for biosynthesized AgNPs. This is the first report on the biosynthesis of AgNPs using leaves extract of HI. Results of present study could contribute to synthesize new and cost effective drugs from HI by using green approach.