Department of Biotechnology

About: Department of Biotechnology is a government organization based out in New Delhi, India. It is known for research contribution in the topics: Population & Silver nanoparticle. The organization has 4800 authors who have published 5033 publications receiving 82022 citations.

Cannabis sativa: An ancient wild edible plant of India -

Abstract: Cannabis sativa, also known as Cannabis indica or Indian hemp, is an annual herb of the family Cannabinaceae. It has been used by humans throughout recorded history for its food, fiber and medicine. It is a native to Central Asia, and long cultivated in Asia, Europe and China. Plants yielding the drug seem to have been discovered in India, cultivated for medicinal purposes as early as 900 BC. Hindu devotees offered Cannabis to Shiva during religious ceremonies, and the herb continues to have a religious association in India. The medicinal use of Cannabis has very long history. However, the sociopolitical pressure led to decline the medicinal use of Cannabis. But despite its illegality, people have continued to obtain Cannabis in black market for self-medication. Together with coffee and tobacco, Cannabis is the most commonly used psychoactive drug worldwide, and it is the single most popular illegal drug. This review analyzes the diversity, botanical description, consumption and pharmacological studies along with medicinal uses of Cannabis among the human being throughout the world.

Photocatalytic degradation of azo dye Orange II in aqueous solutions using copper-impregnated titania

Abstract: During dyeing process, industries consume large quantity of water and subsequently produce large volume of wastewater This wastewater is rich in color and contains different dyes Orange II is one of them In this article, metal-impregnated TiO2 P-25 catalyst was used to enhance the photocatalytic degradation of Orange II dye Photodegradation percentage was followed spectrophotometrically by the measurements of absorbance at λ max = 483 nm The effect of copper-impregnated TiO2 P-25 photocatalyst for the degradation of Orange II has been investigated in terms of percentage removal of color, chemical oxygen demand (COD) and total organic carbon (TOC) As such 98 % color removal efficiency, 97 % percentage removal of COD and 89 % percentage removal of TOC was achieved with TiO2 P-25/Cu catalysts under typical conditions Copper-impregnated TiO2 P-25 photocatalyst showed comparatively higher activity than UV/H2O2 homogeneous photodegradation The relative electrical energy consumption for photocatalytic degradation was considerably lower with TiO2 P-25/Cu photocatalyst than that with homogeneous photodegradation Transmission electron microscopic analysis was used for catalyst characterization

Microbiota-accessible pectic poly- and oligosaccharides in gut health

Abstract: Diverse human intestinal microbiota are regarded as a prerequisite for a healthy intestine. Commercial prebiotic products have a limited ability to provide microbial diversity in the human gut, because they mostly comprise oligomers of the same monosaccharide residues and a small fraction of them can reach the distal colon. Therefore, the demand for diverse prebiotic ingredients and dietary fibers with improved functional properties is increasing tremendously. The main sources of carbohydrates in our diet are plant-derived polysaccharides, which are consumed by the bacteria present in the intestine. Among these, pectin-derived poly- and oligosaccharides serve as the best alternative, as they are resistant to human gastric juice and are fermented slowly in the large intestine to impart a prebiotic effect. The main components of pectin are polygalacturonic acids in association with neutral polysaccharides such as arabinan, arabinogalactan, and galactan. The present review deals with the health-related functional properties of pectic poly- and oligosaccharides and their applications in the food industry. Different mechanisms involved in the hydrolysis of these carbohydrates by the intestinal bacteria and in maintaining the microbial diversity of the intestine are also discussed. It also emphasizes the current methods for the production and purification of different pectins and their oligosaccharides.

A Randomized Double-Blind Placebo-Controlled Phase IIB Trial of Curcumin in Oral Leukoplakia.

Abstract: Oral leukoplakia is a potentially malignant lesion of the oral cavity, for which no effective treatment is available. We investigated the effectiveness of curcumin, a potent inhibitor of NF-κB/COX-2, molecules perturbed in oral carcinogenesis, to treat leukoplakia. Subjects with oral leukoplakia (n = 223) were randomized (1:1 ratio) to receive orally, either 3.6 g/day of curcumin (n = 111) or placebo (n = 112), for 6 months. The primary endpoint was clinical response obtained by bi-dimensional measurement of leukoplakia size at recruitment and 6 months. Histologic response, combined clinical and histologic response, durability and effect of long-term therapy for an additional six months in partial responders, safety and compliance were the secondary endpoints. Clinical response was observed in 75 (67.5%) subjects [95% confidence interval (CI), 58.4-75.6] in the curcumin and 62 (55.3%; 95% CI, 46.1-64.2) in placebo arm (P = 0.03). This response was durable, with 16 of the 18 (88.9%; 95% CI, 67.2-96.9) subjects with complete response in curcumin and 7 of 8 subjects (87.5%) in placebo arm, demonstrating no relapse after 6 months follow-up. Difference in histologic response between curcumin and placebo was not significant (HR, 0.88, 95% CI, 0.45-1.71; P = 0.71). Combined clinical and histologic response assessment indicated a significantly better response with curcumin (HR, 0.50; 95% CI, 0.27-0.92; P = 0.02). Continued therapy, in subjects with partial response at 6 months, did not yield additional benefit. The treatment did not raise any safety concerns. Treatment of oral leukoplakia with curcumin (3.6 g for six months), thus was well tolerated and demonstrated significant and durable clinical response for 6 months. Cancer Prev Res; 9(8); 683-91. ©2016 AACR.

In vitro selection and regeneration of carnation (Dianthus caryophyllus L.) plants resistant to culture filtrate of Fusarium oxysporum f.sp. dianthi

Abstract: Callus cultures derived from internodal segments of two cultivars of carnation susceptible to Fusarium oxysporum f.sp. dianthi were successfully used for in vitro selection for resistance to this pathogenic fungus. Resistant lines were selected by culturing calli on growth medium containing various concentrations of the culture filtrate of F. oxysporum f.sp. dianthi. Resistant calli obtained after two cycles (25 days/cycle) of selection were used for plant regeneration. About 32% of the plants regenerated from the resistant calli had acquired considerable resistance against the pathogen in the field. No phenotypic variation was observed in the selected regenerates.