Department of Biotechnology

About: Department of Biotechnology is a government organization based out in New Delhi, India. It is known for research contribution in the topics: Population & Silver nanoparticle. The organization has 4800 authors who have published 5033 publications receiving 82022 citations.

Distribution, partitioning, bioaccumulation of trace elements in water, sediment and fish from sewage fed fish ponds in eastern Kolkata, India

Abstract: Concentrations of trace elements were measured in water (Cu, Zn, Fe, Pb, Mn, and Al), sediment (Cr, Cu, Mn, Pb, Ni, Al, Zn, Fe, and Co), and muscle tissue of nine selected fish species (Cu, Zn, Fe, and Mn) collected from eastern Kolkata (India) estuarine-sewage fed fish ponds. In water, trace elements existed in particulate phase (60–80%) with Fe as the predominant element followed by Al > Mn > Zn > Pb > Cu. The partitioning coefficients (Kd ) of the trace elements are low and fairly stable. The Pearson product moment correlation among the elements in the particulate and dissolved phase of the water column was calculated and most of the elements are correlated well (p ≤ 0.005). The trace element concentrations in sediments were in the following order: Al ≥ Fe > Mn > Zn > Cu > Ni > Cr > Pb > Co. Contamination factors (CFs) of trace elements in sediments were in the order of Pb ≥ Cu > Zn > Fe > Mn ≥ Ni ≥ Co > Al > Cr and Pollution Load Index ranges were 0.33–0.56. The CFs for Pb, Cu, and Zn are 0.92, 0.88, ...

Nanotitania Exposure Causes Alterations in Physiological, Nutritional and Stress Responses in Tomato ( Solanum lycopersicum)

Abstract: Titanium dioxide nanoparticles (nanotitania: TiO2NPs) are used in a wide range of consumer products, paints, sunscreens and cosmetics. The increased applications lead to the subsequent release of nanomaterials in environment that could affect the plant productivity. However, few studies have been performed to determine the overall effects of TiO2NPs on edible crops. We treated tomato plants with 0.5, 1, 2 and 4 g/L TiO2NPs in a hydroponic system for two weeks and examined physiological, biochemical and molecular changes.. The dual response was observed on growth and photosynthetic ability of plants depending on TiO2NPs concentrations. Low concentrations (0.5–2 g/L) of TiO2NPs boosted growth by approximately 50% and caused significant increase in photosynthetic parameters such as quantum yield, performance index, and total chlorophyll content as well as induced expression of PSI gene with respect to untreated plants . The high concentration (4g/L) affected these parameters in negative manner. The catalase and peroxidase activities were also elevated in the exposed plants in a dose-dependent manner. Likewise, exposed plants exhibited increased expressions of glutathione synthase and glutathione S-transferase (nearly 3-fold increase in both roots and leaves), indicating a promising role of thiols in detoxification of TiO2NPs in tomato. The elemental analysis of tissues performed at 0.5, 1 and 2 g/L TiO2NPs indicates that TiO2NPs transport significantly affected the distribution of essential elements (P, S, Mg and Fe) in roots and leaves displaying about three fold increases in P and 25% decrease in Fe contents. This study presents the mechanistic basis for the differential responses of titanium nanoparticles in tomato, and calls for a cautious approach for the application of nanomaterials in agriculture.

Bioconversion of agricultural waste to ethanol by SSF using recombinant cellulase from Clostridium thermocellum.

Abstract: The effect of different pretreatment methods, temperature, and enzyme concentration on ethanol production from 8 lignocellulosic agrowaste by simultaneous saccharification and fermentation (SSF) using recombinant cellulase and Saccharomyces cerevisiae were studied. Recombinant cellulase was isolated from E. coli BL21 cells transformed with CtLic26A-Cel5-CBM11 full-length gene from Clostridium thermocellum and produced in both batch and fed-batch processes. The maximum cell OD and specific activity in batch mode were 1.6 and 1.91 U/mg, respectively, whereas in the fed-batch mode, maximum cell OD and specific activity were 3.8 and 3.5 U/mg, respectively, displaying a 2-fold increase. Eight substrates, Syzygium cumini (jamun), Azadirachta indica (neem), Saracens indica (asoka), bambusa dendrocalmus (bamboo), Populas nigra (poplar), Achnatherum hymenoides (wild grass), Eucalyptus marginata (eucalyptus), and Mangifera indica (mango), were subjected to SSF. Of three pretreatments, acid, alkali, and steam explosion, acid pretreatment Syzygium cumini (Jamun) at 30°C gave maximum ethanol yield of 1.42 g/L.

Cognitive dysfunction: A growing link between diabetes and Alzheimer's disease.

Abstract: Diabetes mellitus (DM) is a gradually rising metabolic disease which is currently affecting millions of people worldwide. Diabetes is associated with various complications like nephropathy, neuropathy, retinopathy, diabetic foot, cognitive impairment, and many more. Evidence suggests that cognitive dysfunction is a rising complication of diabetes which adversely affects the brain of patients suffering from diabetes. Age-related memory impairment is a complication having its major effect on people suffering from diabetes and Alzheimer's. Patients suffering from diabetes are at two times higher risk of developing cognitive dysfunction as compared with normal individuals. Multiple factors which are involved in diabetes related complications are found to play a role in the development of neurodegeneration in Alzheimer's. The problem of insulin deficiency and insulin resistance is well reported in diabetes but there are many studies which suggest dysregulation of insulin levels as a reason behind the development of Alzheimer's. As the link between diabetes and Alzheimer disease (AD) is deepening, there is a need to understand the plausible tie-ins between the two. Emerging role of major factors like insulin imbalance, advanced glycation end products and micro-RNA's involved in diabetes and Alzheimer's have been discussed here. This review helps in understanding the plausible mechanism underlying the pathophysiology of amyloid beta (Aβ) plaque formation and tau hyperphosphorylation as well provides information about studies carried out in this area of research. The final thought is to enhance the scientific knowledge on this correlation and develop future therapeutics to treat the same.