Department of Biotechnology

About: Department of Biotechnology is a government organization based out in New Delhi, India. It is known for research contribution in the topics: Population & Silver nanoparticle. The organization has 4800 authors who have published 5033 publications receiving 82022 citations.

Mitochondrial Control Region Alterations and Breast Cancer Risk: A Study in South Indian Population

Abstract: Background Mitochondrial displacement loop (D-loop) is the hot spot for mitochondrial DNA (mtDNA) alterations which influence the generation of cellular reactive oxygen species (ROS). Association of D-loop alterations with breast cancer has been reported in few ethnic groups; however none of the reports were documented from Indian subcontinent. Methodology We screened the entire mitochondrial D-loop region (1124 bp) of breast cancer patients (n = 213) and controls (n = 207) of south Indian origin by PCR-sequencing analysis. Haplotype frequencies for significant loci, the standardized disequilibrium coefficient (D') for pair-wise linkage disequilibrium (LD) were assessed by Haploview Software. Principal findings We identified 7 novel mutations and 170 reported polymorphisms in the D-loop region of patients and/or controls. Polymorphisms were predominantly located in hypervariable region I (60%) than in II (30%) of D-loop region. The frequencies of 310'C' insertion (P = 0.018), T16189C (P = 0.0019) variants and 310'C'ins/16189C (P = 0.00019) haplotype were significantly higher in cases than in controls. Furthermore, strong LD was observed between nucleotide position 310 and 16189 in controls (D' = 0.49) as compared to patients (D' = 0.14). Conclusions Mitochondrial D-loop alterations may constitute inherent risk factors for breast cancer development. The analysis of genetic alterations in the D-loop region might help to identify patients at high risk for bad progression, thereby helping to refine therapeutic decisions in breast cancer.

Label-Free Electrochemical Immunosensor Based on One-Step Electrochemical Deposition of AuNP-RGO Nanocomposites for Detection of Endometriosis Marker CA 125

Abstract: Endometriosis is the third most prominent gynecological disorder. Cancer antigen 125 (CA 125) is the primary serum marker used for late-stage endometriosis diagnosis and management. Herein, we deve...

Triphenylamine-based stimuli-responsive solid state fluorescent materials

Abstract: Smart fluorescent materials exhibit a controlled fluorescence response to different external stimuli such as pressure, heat, light, pH, etc. Molecular structure and supramolecular assembly via weak intermolecular interactions strongly influence the fluorescence efficiency and colour. In particular, non-planar molecular structures play a significant role in developing solid state fluorescent materials. Triphenylamine (TPA), a typical non-planar propeller molecule with interesting optoelectronic properties, provides an excellent opportunity for developing a variety of molecular fluorescent materials by taking advantage of synthetic feasibility. For example, a donor–acceptor (D–A) aggregation induced emissive (AIE) fluorophore can be developed by integrating an acceptor group into the phenyl unit of a TPA donor. In this review, we focus exclusively on TPA-based smart fluorescent materials that exhibit distinct and reversible fluorescence switching towards different stimuli. Molecular engineering of the TPA fluorophore resulted in the development of different types of stimuli-responsive materials and the conformational flexibility of non-planar phenyl groups often produced polymorphism induced fluorescence tuning. A detailed discussion of the structure–property relationships of TPA derivatives could provide insights for developing new TPA-based smart fluorescent materials with versatile properties.