Showing papers by "Drexel University published in 2001"

Dendritic spine geometry is critical for AMPA receptor expression in hippocampal CA1 pyramidal neurons

Abstract: Dendritic spines serve as preferential sites of excitatory synaptic connections and are pleomorphic. To address the structure-function relationship of the dendritic spines, we used two-photon uncaging of glutamate to allow mapping of functional glutamate receptors at the level of the single synapse. Our analyses of the spines of CA1 pyramidal neurons reveal that AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid)-type glutamate receptors are abundant (up to 150/spine) in mushroom spines but sparsely distributed in thin spines and filopodia. The latter may be serving as the structural substrates of the silent synapses that have been proposed to play roles in development and plasticity of synaptic transmission. Our data indicate that distribution of functional AMPA receptors is tightly correlated with spine geometry and that receptor activity is independently regulated at the level of single spines.

Modeling and control of formations of nonholonomic mobile robots

Abstract: This paper addresses the control of a team of nonholonomic mobile robots navigating in a terrain with obstacles while maintaining a desired formation and changing formations when required, using graph theory. We model the team as a triple, (g, r, H), consisting of a group element g that describes the gross position of the lead robot, a set of shape variables r that describe the relative positions of robots, and a control graph H that describes the behaviors of the robots in the formation. Our framework enables the representation and enumeration of possible control graphs and the coordination of transitions between any two formations.

Spectroscopic Target Selection for the Sloan Digital Sky Survey: The Luminous Red Galaxy Sample

Abstract: We describe the target selection and resulting properties of a spectroscopic sample of luminous red galaxies (LRGs) from the imaging data of the Sloan Digital Sky Survey (SDSS). These galaxies are selected on the basis of color and magnitude to yield a sample of luminous intrinsically red galaxies that extends fainter and farther than the main flux-limited portion of the SDSS galaxy spectroscopic sample. The sample is designed to impose a passively evolving luminosity and rest-frame color cut to a redshift of 0.38. Additional, yet more luminous red galaxies are included to a redshift of ~0.5. Approximately 12 of these galaxies per square degree are targeted for spectroscopy, so the sample will number over 100,000 with the full survey. SDSS commissioning data indicate that the algorithm efficiently selects luminous (M^+_g ≈ -21.4) red galaxies, that the spectroscopic success rate is very high, and that the resulting set of galaxies is approximately volume limited out to z = 0.38. When the SDSS is complete, the LRG spectroscopic sample will fill over 1 h^(-3) Gpc^3 with an approximately homogeneous population of galaxies and will therefore be well suited to studies of large-scale structure and clusters out to z = 0.5.

Sp1 and Krüppel-like factor family of transcription factors in cell growth regulation and cancer

Abstract: The Sp/KLF family contains at least twenty identified members which include Sp1-4 and numerous kruppel-like factors. Members of the family bind with varying affinities to sequences designated as ‘Sp1 sites’ (e.g., GC-boxes, CACCC-boxes, and basic transcription elements). Family members have different transcriptional properties and can modulate each other's activity by a variety of mechanisms. Since cells can express multiple family members, Sp/KLF factors are likely to make up a transcriptional network through which gene expression can be fine-tuned. ‘Sp1 site’-dependent transcription can be growth-regulated, and the activity, expression, and/or post-translational modification of multiple family members is altered with cell growth. Furthermore, Sp/KLF factors are involved in many growth-related signal transduction pathways and their overexpression can have positive or negative effects on proliferation. In addition to growth control, Sp/KLF factors have been implicated in apoptosis and angiogenesis; thus, the family is involved in several aspects of tumorigenesis. Consistent with a role in cancer, Sp/KLF factors interact with oncogenes and tumor suppressors, they can be oncogenic themselves, and altered expression of family members has been detected in tumors. Effects of changes in Sp/KLF factors are context-dependent and can appear contradictory. Since these factors act within a network, this diversity of effects may arise from differences in the expression profile of family members in various cells. Thus, it is likely that the properties of the overall network of Sp/KLF factors play a determining role in regulation of cell growth and tumor progression. © 2001 Wiley-Liss, Inc.

Validation in information systems research: a state-of-the-art assessment

Abstract: Over 10 years ago, the issue of whether IS researchers were rigorously validating their quantitative, positivist instruments was raised (Straub 1989). In the years that have passed since that time, the profession has undergone many changes. Novel technologies and management trends have come and gone. New professional societies have been formed and grown in prominence and new demands have been placed on the field's research and teaching obligations. But the issue of rigor in IS research has persisted throughout all such changes. Without solid validation of the instruments that are used to gather data upon which findings and interpretations are based, the very scientific basis of positivist, quantitative research is threatened. As a retrospective on the Straub article, this research seeks to determine if and how the field has advanced in instrument validation. As evidence of the change, we coded positivist, quantitative research articles in five major journals over a recent three year period for use of validation techniques. Findings suggest that the field has advanced in many areas, but, overall, it appears that a majority of published studies are still not sufficiently validating their instruments. Based on these findings, approaches are suggested for reinvigorating the quest for validation in IS research via content/construct validity, reliability, and manipulation validity.

The Prediction of Criminal Recidivism in Juveniles A Meta-Analysis

Abstract: A meta-analysis was conducted to identify risk factors that best predict juvenile recidivism, defined as rearrest for offending of any kind. Twenty-three published studies, representing 15,265 juveniles, met inclusion criteria. Effect sizes were calculated for 30 predictors of recidivism. Eight groups of predictors were compared: (a) demographic information, (b) offense history, (c) family and social factors, (d) educational factors, (e) intellectual and achievement scores, (f) substance use history, (g) clinical factors, and (h) formal risk assessment. The domain of offense history was the strongest predictor of reoffending. Other relatively strong predictors included family problems, ineffective use of leisure time, delinquent peers, conduct problems, and nonsevere pathology.

Type of employment, work–family conflict and well-being: a comparative study

Abstract: The study examined the impact of work and family role characteristics on work–family conflict, and indicators of psychological well being among self-employed and organizationally employed women and men. Results show that employment type and gender have independent main effects on several of the study variables. Self-employed persons enjoy greater autonomy and schedule flexibility at work, and report higher levels of job involvement and job satisfaction than those employed in organizations. However, they also experience higher levels of work–family conflict, and lower family satisfaction than organizational employees. The findings suggest that there are trade-offs between the costs and benefits of self-employment, and that business ownership is not a panacea for balancing work and family role responsibilties. Directions for future research are discussed. Copyright © 2001 John Wiley & Sons, Ltd.

The Luminosity Function of Galaxies in SDSS Commissioning Data

Abstract: In the course of its commissioning observations, the Sloan Digital Sky Survey (SDSS) has produced one of the largest redshift samples of galaxies selected from CCD images. Using 11,275 galaxies complete to r* \ 17.6 over 140 deg2, we compute the luminosity function of galaxies in the r* band over a range (for h \ 1). The result is well-described by a Schechter function with parameters [23 \ M rp \ [16 h3 Mpc~3,

Development and application of triglyceride‐based polymers and composites

Abstract: Triglyceride oils derived from plants have been used to synthesize several different monomers for use in structural applications. These monomers have been found to form polymers with a wide range of physical properties. They exhibit tensile moduli in the 1–2 GPa range and glass transition temperatures in the range 70–120 °C, depending on the particular monomer and the resin composition. Composite materials were manufactured utilizing these resins and produced a variety of durable and strong materials. At low glass fiber content (35 wt %), composites produced from acrylated epoxidized soybean oil by resin transfer molding displayed a tensile modulus of 5.2 GPa, a flexural modulus of 9 GPa, a tensile strength of 129 MPa, and flexural strength of 206 MPa. At higher fiber contents (50 wt %) composites produced from acrylated epoxidized soybean oil displayed tensile and compression moduli of 24.8 GPa each, and tensile and compressive strengths of 463.2 and 302.6 MPa, respectively. In addition to glass fibers, natural fibers such as flax and hemp were used. Hemp composites of 20% fiber content displayed a tensile strength of 35 MPa and a tensile modulus of 4.4 GPa. The flexural modulus was ∼2.6 GPa and the flexural strength was in the range 35.7–51.3 MPa, depending on the test conditions. The flax composite materials had tensile and flexural strengths in the ranges 20–30 and 45–65 MPa, respectively. The properties exhibited by both the natural- and synthetic fiber-reinforced composites can be combined through the production of “hybrid” composites. These materials combine the low cost of natural fibers with the high performance of synthetic fibers. Their properties lie between those displayed by the all-glass and all-natural composites. Characterization of the polymer properties also presents opportunities for improvement through genetic engineering technology. © 2001 John Wiley & Sons, Inc. J Appl Polym Sci 82: 703–723, 2001

Dynamic Measurements of Three-Dimensional Scapular Kinematics: A Validation Study

Abstract: The validation of two noninvasive methods for measuring the dynamic three-dimensional kinematics of the human scapula with a magnetic tracking device is presented. One method consists of simply fixing a sensor directly to the acromion and the other consists of mounting a sensor to an adjustable plastic jig that fits over the scapular spine and acromion. The concurrent validity of both methods was assessed separately by comparison with data collected simultaneously from an invasive approach in which pins were drilled directly into the scapula. The differences between bone and skin based measurements represents an estimation of skin motion artifact. The average motion pattern of each surface method was similar to that measured by the invasive technique, especially below 120 degrees of elevation. These results indicate that with careful consideration, both methods may offer reasonably accurate representations of scapular motion that may be used to study shoulder pathologies and help develop computational models.

The role of matrix metalloproteinases in tumor angiogenesis and tumor metastasis.

Abstract: Although a considerable amount of effort has been placed on discovering the etiologies of cancer, the majority of the basic cancer research existing today has focused on understanding the molecular mechanism of tumor formation and metastasis. Metastatic spread of tumors continues to be a major obstacle to successful treatment of malignant tumors. Approximately 30% of those patients diagnosed with a solid tumor have a clinically detectable metastasis and for the remaining 70%, metastases are continually being formed throughout the life of the tumor. Even after the tumor is excised, the threat of death is attributable to the metastasis that may occur through the remaining tumor cells. In addition, treating the metastasis often proves futile since metastasis often vary in size, composition, and anatomical location. New treatments blocking the formation of metastasis will provide greater chances of survival for cancer patients. One family of enzymes that has been shown over the years to play a role in tumor progression is the matrix metalloproteinase (MMP) family. The main function of MMPs, also known as matrixins, is degradation of the extracellular matrix physiologic function involving MMPs include wound healing, bone resorption and mammary involution. MMPs, however, also contribute to pathological conditions including rheumatoid arthritis, coronary artery disease, and cancer. Tumor cells are believed to utilize the matrix degrading capability of these enzymes to spread to distant sites. In addition, MMPs also are thought to promote the growth of these tumor cells once they have metastasized. This review will discuss the role of MMPs and their inhibitors in tumor invasion, angiogenesis and metastasis with special emphasis on the gelatinases, MMP-2 and MMP-9.

A painful peripheral neuropathy in the rat produced by the chemotherapeutic drug, paclitaxel.

Abstract: Paclitaxel, an effective anti-neoplastic agent in the treatment of solid tumors, produces a dose-limiting painful peripheral neuropathy in a clinically significant number of cancer patients. Prior work has demonstrated paclitaxel-induced neurodegeneration and sensory loss in laboratory rodents. We describe here an experimental paclitaxel-induced painful peripheral neuropathy. Adult male rats were given four intraperitoneal injections on alternate days of vehicle or 0.5, 1.0, or 2.0 mg/kg of paclitaxel (Taxol). Behavioral tests for pain using mechanical and thermal stimuli applied to the tail and hind paws, and tests for motor performance, were taken before, during and after dosing for 22-35 days. All three doses of paclitaxel caused heat-hyperalgesia, mechano-allodynia, mechano-hyperalgesia, and cold-allodynia, but had no effect on motor performance. Neuropathic pain began within days and lasted for several weeks. We did not detect any dose-response relationship. Tests at the distal, mid, and proximal tail failed to show evidence of a length-dependent neuropathy. Vehicle control injections had no effect on any measure. No significant systemic toxicities were noted in the paclitaxel-treated animals. Light-microscopic inspection of the sciatic nerve (mid-thigh level), L4-L5 dorsal root ganglia, and dorsal and ventral roots, and the gray and white matter of the L4-L5 spinal cord, showed no structural abnormalities. Electron microscopic examination of the sciatic nerve (mid-thigh level) and the L4-L5 dorsal root ganglia and dorsal horns demonstrated no degeneration of myelinated and unmyelinated axons in the sciatic nerve and roots, but revealed endoneurial edema. This model may be useful in understanding a significant source of pain in cancer patients, and in finding ways to avoid the neurotoxicity that limits paclitaxel therapy.

The Global Internet Shopper: Evidence from Shopping Tasks in Twelve Countries

Abstract: Globally, the characteristics of a website that are critical to increasing the likelihood that customers will shop at that site and will come back for future purchases are largely unknown. Actual shopping tasks by 299 respondents from 12 countries indicate that site quality, trust, and positive affect toward it are critical in explaining both the purchase intentions and loyalty of visitors to the site. This research indicates that the impact of these factors varies across different regions of the world and across different product categories. Results of this research highlight the need to tailor websites according to each world region and product being offered for sale.

Mesoporous Aluminosilicates with Ordered Hexagonal Structure, Strong Acidity, and Extraordinary Hydrothermal Stability at High Temperatures

Abstract: Highly ordered hexagonal mesoporous aluminosilicates (MAS-5) with uniform pore sizes have been successfully synthesized from assembly of preformed aluminosilcate precursors with cetyltrimethylammonium bromide (CTAB) surfactant. The aluminosilicate precursors were obtained by heating, at 100−140 °C for 2−10 h, aluminasilica gels at the Al2O3/SiO2/TEAOH/H2O molar ratios of 1.0/7.0−350/10.0−33.0/500−2000. Mesoporous MAS-5 shows extraordinary stability both in boiling water (over 300 h) and in steam (800 °C for 2 h). Temperature-programmed desorption of ammonia shows that the acidic strength of MAS-5 is much higher than that of MCM-41 and is comparable to that of microporous Beta zeolite. In catalytic cracking of 1,3,5-triisopropylbenzene and alkylation of isobutane with butene, MAS-5 exhibits greater catalytic activity and selectivity, as compared with MCM-41 and HZSM-5. The MAS-5 samples were characterized with infrared, UV−Raman, and NMR spectroscopy and numerous other techniques. The results suggest that ...

Using unknowns to prevent discovery of association rules

Abstract: Data mining technology has given us new capabilities to identify correlations in large data sets. This introduces risks when the data is to be made public, but the correlations are private. We introduce a method for selectively removing individual values from a database to prevent the discovery of a set of rules, while preserving the data for other applications. The efficacy and complexity of this method are discussed. We also present an experiment showing an example of this methodology.

Career involvement and family involvement as moderators of relationships between work-family conflict and withdrawal from a profession.

Abstract: This study extended prior analyses by J. H. Greenhaus, K. M. Collins, R. Singh, and S. Parasuraman (1997) by examining relationships between 2 directions of work-family conflict (work-to-family conflict and family-to-work conflict) and withdrawal from public accounting. The sample consisted of 199 members of the American Institute of Certified Public Accountants (135 men and 64 women) who were married or in a long-term relationship and who had 1 or more children. It was found that work-to-family conflict (but not family-to-work conflict) was positively related to withdrawal intentions. In addition, relationships of work-to-family conflict with withdrawal intentions and withdrawal behavior were stronger for individuals who were relatively uninvolved in their careers than for those who were highly involved in their careers. The implications of the findings for future research are discussed.

Hiding Association Rules by Using Confidence and Support

Abstract: Large repositories of data contain sensitive information which must be protected against unauthorized access. Recent advances, in data mining and machine learning algorithms, have increased the disclosure risks one may encounter when releasing data to outside parties. A key problem, and still not sufficiently investigated, is the need to balance the confidentiality of the disclosed data with the legitimate needs of the data users. Every disclosure limitation method affects, in some way, and modifies true data values and relationships. In this paper, we investigate confidentiality issues of a broad category of rules, which are called association rules. If the disclosure risk of some of these rules are above a certain privacy threshold, those rules must be characterized as sensitive. Sometimes, sensitive rules should not be disclosed to the public since, among other things, they may be used for inferencing sensitive data, or they may provide business competitors with an advantage.

Annexin-V imaging for noninvasive detection of cardiac allograft rejection

Abstract: Heart transplant rejection is characterized pathologically by myocyte necrosis and apoptosis associated with interstitial mononuclear cell infiltration. Any one of these components can be targeted for noninvasive detection of transplant rejection. During apoptotic cell death, phosphatidylserine, a phospholipid that is normally confined to the inner leaflet of cell membrane bilayer, gets exteriorized. Technetium-99m-labeled annexin-V, an endogenous protein that has high affinity for binding to phosphatidylserine, has been administered intravenously for noninvasive identification of apoptotic cell death. In the present study of 18 cardiac allograft recipients, 13 patients had negative and five had positive myocardial uptake of annexin. These latter five demonstrated at least moderate transplant rejection and caspase-3 staining, suggesting apoptosis in their biopsy specimens. This study reveals the clinical feasibility and safety of annexin-V imaging for noninvasive detection of transplant rejection by targeting cell membrane phospholipid alterations that are commonly associated with the process of apoptosis.

Prediction of protein interaction sites from sequence profile and residue neighbor list

Abstract: Protein-protein interaction sites are predicted from a neural network with sequence profiles of neighboring residues and solvent exposure as input. The network was trained on 615 pairs of nonhomologous complex-forming proteins. Tested on a different set of 129 pairs of nonhomologous complex-forming proteins, 70% of the 11,004 predicted interface residues are actually located in the interfaces. These 7732 correctly predicted residues account for 65% of the 11,805 residues making up the 129 interfaces. The main strength of the network predictor lies in the fact that neighbor lists and solvent exposure are relatively insensitive to structural changes accompanying complex formation. As such, it performs equally well with bound or unbound structures of the proteins. For a set of 35 test proteins, when the input was calculated from the bound and unbound structures, the correct fractions of the predicted interface residues were 69 and 70%, respectively.

Missing Link Found? The "Runaway" Path to Supermassive Black Holes

Abstract: Observations of stellar kinematics, gasdynamics, and masers around galactic nuclei have now firmly established that many galaxies host central supermassive black holes (SMBHs) with masses in the range of ~106-109 M☉. However, how these SMBHs formed is not well understood. One reason for this situation is the lack of observations of intermediate-mass BHs (IMBHs), which could bridge the gap between stellar mass BHs and SMBHs. Recently, this missing link (i.e., an IMBH) has been found in observations made by ASCA and Chandra of the central region of the starburst galaxy M82. Subsequent observations by Subaru have revealed that this IMBH apparently coincides with a young compact star cluster. Based on these findings, we suggest a new formation scenario for SMBHs. In this scenario, IMBHs first form in young compact star clusters through runaway merging of massive stars. While these IMBHs are forming, the host star clusters sink toward the galactic nucleus through dynamical friction and upon evaporation deposit their IMBHs near the galactic center. The IMBHs then form binaries and eventually merge via gravitational radiation, forming an SMBH.

Galaxy Number Counts from the Sloan Digital Sky Survey Commissioning Data

Abstract: We present bright galaxy number counts in five broad bands (u', g', r', i', z') from imaging data taken during the commissioning phase of the Sloan Digital Sky Survey (SDSS). The counts are derived from two independent stripes of imaging scans along the celestial equator, one each toward the northern and the southern Galactic cap, covering about 230 and 210 deg2, respectively. A careful study is made to verify the reliability of the photometric catalog. For galaxies brighter than r* = 16, the catalog produced by automated software is examined against eye inspection of all objects. Statistically meaningful results on the galaxy counts are obtained in the magnitude range 12 ≤ r* ≤ 21, using a sample of 900,000 galaxies. The counts from the two stripes differ by about 30% at magnitudes brighter than r* = 15.5, consistent with a local 2 σ fluctuation due to large-scale structure in the galaxy distribution. The shape of the number counts–magnitude relation brighter than r* = 16 is well characterized by N ∝ 100.6m, the relation expected for a homogeneous galaxy distribution in a "Euclidean" universe. In the magnitude range 16 < r* < 21, the galaxy counts from both stripes agree very well and follow the prediction of the no-evolution model, although the data do not exclude a small amount of evolution. We use empirically determined color transformations to derive the galaxy number counts in the B and I814 bands. We compute the luminosity density of the universe at zero redshift in the five SDSS bands and in the B band. We find B = 2.4 ± 0.4 × 108 L⊙ h Mpc-3, for a reasonably wide range of parameters of the Schechter luminosity function in the B band.

Identification of a novel common genetic risk factor for lumbar disk disease.

Abstract: ContextLumbar disk disease (LDD) is one of the most common musculoskeletal diseases, with a prevalence of about 5%. A tryptophan (Trp) allele (Trp2) was recently discovered in the COL9A2 gene that is associated with dominantly inherited LDD but is only present in about 4% of Finnish patients with LDD.ObjectiveTo determine if other collagen IX gene sequence variations play a role in the pathogenesis of LDD.Design and SettingCase-control study conducted from February 1997 to May 1998 at university hospitals in Finland.ParticipantsA total of 171 individuals with LDD (evaluated clinically and by magnetic resonance imaging or computed tomography) and 321 controls without LDD (186 healthy individuals, 83 patients with primary osteoarthritis, 31 with rheumatoid arthritis, and 21 with chondrodysplasias).Main Outcome MeasuresFrequencies of sequence variations covering the entire coding sequences and exon boundaries of the collagen IX genes, COL9A1, COL9A2, and COL9A3, which code for the α1, α2, and α3 chains of the protein, detected by conformation-sensitive gel electrophoresis and confirmed by sequencing, compared between individuals with and without LDD.ResultsMutation analysis of all 3 collagen IX genes resulted in identification of an Arg103→Trp (arginine→tryptophan) substitution in the α3 chain (Trp3 allele). The frequency of the Trp3 allele was 12.2% in LDD cases, excluding 7 individuals who were carriers of the previously identified Gln326→Trp (glutamine→tryptophan) substitution in the α2 chain (Trp2 allele), and was 4.7% among controls. The difference in the frequency was statistically significant (P = .000013). Presence of at least 1 Trp3 allele increases risk of LDD about 3-fold.ConclusionThis study led to the identification of a novel common genetic risk factor for LDD, confirming that genetic risk factors likely play a significant role in LDD.

Bone tissue engineering in a rotating bioreactor using a microcarrier matrix system.

Abstract: A novel approach was utilized to grow in vitro mineralized bone tissue using lighter-than-water, polymeric scaffolds in a high aspect ratio rotating bioreactor. We have adapted polymer microencapsulation methods for the formation of hollow, lighter-than-water microcarriers of degradable poly(lactic-co-glycolic acid). Scaffolds were fabricated by sintering together lighter-than-water microcarriers from 500 to 860 μm in diameter to create a fully interconnected, three-dimensional network with an average pore size of 187 μm and aggregate density of 0.65 g/mL. Motion in the rotating bioreactor was characterized by numerical simulation and by direct measurement using an in situ particle tracking system. Scaffold constructs established a near circular trajectory in the fluid medium with a terminal velocity of 98 mm/s while avoiding collision with the bioreactor wall. Preliminary cell culture studies on these scaffolds show that osteoblast-like cells readily attached to microcarrier scaffolds using controlled seeding conditions with an average cell density of 6.5 × 104 cells/cm2. The maximum shear stress imparted to attached cells was estimated to be 3.9 dynes/cm2. In addition, cells cultured in vitro on these lighter-than-water scaffolds retained their osteoblastic phenotype and showed significant increases in alkaline phosphatase expression and alizarin red staining by day 7 as compared with statically cultured controls. © 2001 John Wiley & Sons, Inc. J Biomed Mater Res 55: 242–253, 2001

Classification of ultrasonic B-mode images of breast masses using Nakagami distribution

Abstract: The Nakagami distribution was proposed recently for modeling the echo from tissue. In vivo breast data collected from patients with lesions were studied using this Nakagami model. Chi-square tests showed that the Nakagami distribution is a better fit to the envelope than the Rayleigh distribution. Two parameters, m (effective number) and /spl alpha/ (effective cross section), associated with the Nakagami distribution were used for the classification of breast masses. Data from 52 patients with breast masses/lesions were used in the studies. Receiver operating characteristics were calculated for the classification methods based on these two parameters. The results indicate that these parameters of the Nakagami distribution may be useful in classification of the breast abnormalities. The Nakagami distribution may be a reasonable means to characterize the backscattered echo from breast tissues toward a goal of an automated scheme for separating benign and malignant breast masses.