Dublin City University

About: Dublin City University is a education organization based out in Dublin, Ireland. It is known for research contribution in the topics: Machine translation & Laser. The organization has 5904 authors who have published 17178 publications receiving 389376 citations. The organization is also known as: National Institute for Higher Education, Dublin & DCU.

Multisubstrate-compatible ELISA procedures for rapid and high-sensitivity immunoassays

Abstract: This protocol describes an improved and optimized approach to develop rapid and high-sensitivity ELISAs by covalently immobilizing antibody on chemically modified polymeric surfaces. The method involves initial surface activation with KOH and an O(2) plasma, and then amine functionalization with 3-aminopropyltriethoxysilane. The second step requires covalent antibody immobilization on the aminated surface, followed by ELISA. The ELISA procedure developed is 16-fold more sensitive than established methods. This protocol could be used generally as a quantitative analytical approach to perform high-sensitivity and rapid assays in clinical situations, and would provide a faster approach to screen phage-displayed libraries in antibody development facilities. The antibody immobilization procedure is of ∼3 h duration and facilitates rapid ELISAs. This method can be used to perform assays on a wide range of commercially relevant solid support matrices (including those that are chemically inert) with various biosensor formats.

Carbon Trading: A Critical Conversation on Climate Change, Privatisation and Power

Abstract: Carbon Trading: A Critical Conversation on Climate Change, Privatisation and Powerby Larry Lohmann; Uddevalla: Dag Hammersjold Foundation, The Durban Group for Climate Justice and The Corner House,...

Seven myths of global talent management

Abstract: The challenges associated with managing talent on a global scale are greater than those faced by organisations operating on a domestic scale. We believe that the former relate to the fact that a number of key myths regarding talent management may undermine talent management's contribution to multinational corporation effectiveness and retard the development of management practice in this regard. Our aim is to unpack some of those myths and offer some suggestions for advancing the practice of talent management on the basis of insights from both practice and academic thinking in this area.