Showing papers by "École Polytechnique Fédérale de Lausanne published in 2000"

Engineering the Provitamin A (β-Carotene) Biosynthetic Pathway into (Carotenoid-Free) Rice Endosperm

Abstract: Rice (Oryza sativa), a major staple food, is usually milled to remove the oil-rich aleurone layer that turns rancid upon storage, especially in tropical areas. The remaining edible part of rice grains, the endosperm, lacks several essential nutrients, such as provitamin A. Thus, predominant rice consumption promotes vitamin A deficiency, a serious public health problem in at least 26 countries, including highly populated areas of Asia, Africa, and Latin America. Recombinant DNA technology was used to improve its nutritional value in this respect. A combination of transgenes enabled biosynthesis of provitamin A in the endosperm.

Sampling-50 years after Shannon

Abstract: This paper presents an account of the current state of sampling, 50 years after Shannon's formulation of the sampling theorem. The emphasis is on regular sampling, where the grid is uniform. This topic has benefitted from a strong research revival during the past few years, thanks in part to the mathematical connections that were made with wavelet theory. To introduce the reader to the modern, Hilbert-space formulation, we reinterpret Shannon's sampling procedure as an orthogonal projection onto the subspace of band-limited functions. We then extend the standard sampling paradigm for a presentation of functions in the more general class of "shift-in-variant" function spaces, including splines and wavelets. Practically, this allows for simpler-and possibly more realistic-interpolation models, which can be used in conjunction with a much wider class of (anti-aliasing) prefilters that are not necessarily ideal low-pass. We summarize and discuss the results available for the determination of the approximation error and of the sampling rate when the input of the system is essentially arbitrary; e.g., nonbandlimited. We also review variations of sampling that can be understood from the same unifying perspective. These include wavelets, multiwavelets, Papoulis generalized sampling, finite elements, and frames. Irregular sampling and radial basis functions are briefly mentioned.

Neurodegeneration prevented by lentiviral vector delivery of GDNF in primate models of Parkinson's disease.

Abstract: Lentiviral delivery of glial cell line-derived neurotrophic factor (lenti-GDNF) was tested for its trophic effects upon degenerating nigrostriatal neurons in nonhuman primate models of Parkinson's disease (PD). We injected lenti-GDNF into the striatum and substantia nigra of nonlesioned aged rhesus monkeys or young adult rhesus monkeys treated 1 week prior with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Extensive GDNF expression with anterograde and retrograde transport was seen in all animals. In aged monkeys, lenti-GDNF augmented dopaminergic function. In MPTP-treated monkeys, lenti-GDNF reversed functional deficits and completely prevented nigrostriatal degeneration. Additionally, lenti-GDNF injections to intact rhesus monkeys revealed long-term gene expression (8 months). In MPTP-treated monkeys, lenti-GDNF treatment reversed motor deficits in a hand-reach task. These data indicate that GDNF delivery using a lentiviral vector system can prevent nigrostriatal degeneration and induce regeneration in primate models of PD and might be a viable therapeutic strategy for PD patients.

Water activity as the determinant for homogeneous ice nucleation in aqueous solutions

Abstract: The unique properties of water in the supercooled (metastable) state are not fully understood. In particular, the effects of solutes and mechanical pressure on the kinetics of the liquid-to-solid phase transition of supercooled water and aqueous solutions to ice have remained unresolved. Here we show from experimental data that the homogeneous nucleation of ice from supercooled aqueous solutions is independent of the nature of the solute, but depends only on the water activity of the solution--that is, the ratio between the water vapour pressures of the solution and of pure water under the same conditions. In addition, we show that the presence of solutes and the application of pressure have a very similar effect on ice nucleation. We present a thermodynamic theory for homogeneous ice nucleation, which expresses the nucleation rate coefficient as a function of water activity and pressure. Recent observations from clouds containing ice are in good agreement with our theory and our results should help to overcome one of the main weaknesses of numerical models of the atmosphere, the formulation of cloud processes.

Spatial filtering for zero-order and twin-image elimination in digital off-axis holography

Abstract: Off-axis holograms recorded with a CCD camera are numerically reconstructed with a calculation of scalar diffraction in the Fresnel approximation. We show that the zero order of diffraction and the twin image can be digitally eliminated by means of filtering their associated spatial frequencies in the computed Fourier transform of the hologram. We show that this operation enhances the contrast of the reconstructed images and reduces the noise produced by parasitic reflections reaching the hologram plane with an incidence angle other than that of the object wave.

Rapid changes in the hydrologic cycle of the tropical Atlantic during the last glacial.

Abstract: Sedimentary time series of color reflectance and major element chemistry from the anoxic Cariaco Basin off the coast of northern Venezuela record large and abrupt shifts in the hydrologic cycle of the tropical Atlantic during the past 90,000 years. Marine productivity maxima and increased precipitation and riverine discharge from northern South America are closely linked to interstadial (warm) climate events of marine isotope stage 3, as recorded in Greenland ice cores. Increased precipitation at this latitude during interstadials suggests the potential for greater moisture export from the Atlantic to Pacific, which could have affected the salinity balance of the Atlantic and increased thermohaline heat transport to high northern latitudes. This supports the notion that tropical feedbacks played an important role in modulating global climate during the last glacial period.

Ferroelectric thin films for micro-sensors and actuators: a review

Abstract: This paper reviews deposition, integration, and device fabrication of ferroelectric PbZrxTi1-xO3 (PZT) films for applications in microelectromechanical systems. As examples, a piezoelectric ultrasonic micromotor and pyroelectric infrared detector array are presented. A summary of the published data on the piezoelectric properties of PZT thin films is given. The figures of merit for various applications are discussed. Some considerations and results on operation, reliability, and depolarization of PZT thin films are presented.

Interpolation revisited [medical images application]

Abstract: Based on the theory of approximation, this paper presents a unified analysis of interpolation and resampling techniques. An important issue is the choice of adequate basis functions. The authors show that, contrary to the common belief, those that perform best are not interpolating. By opposition to traditional interpolation, the authors call their use generalized interpolation; they involve a prefiltering step when correctly applied. The authors explain why the approximation order inherent in any basis function is important to limit interpolation artifacts. The decomposition theorem states that any basis function endowed with approximation order ran be expressed as the convolution of a B spline of the same order with another function that has none. This motivates the use of splines and spline-based functions as a tunable way to keep artifacts in check without any significant cost penalty. The authors discuss implementation and performance issues, and they provide experimental evidence to support their claims.

Optimization of mutual information for multiresolution image registration

Abstract: We propose a new method for the intermodal registration of images using a criterion known as mutual information. Our main contribution is an optimizer that we specifically designed for this criterion. We show that this new optimizer is well adapted to a multiresolution approach because it typically converges in fewer criterion evaluations than other optimizers. We have built a multiresolution image pyramid, along with an interpolation process, an optimizer, and the criterion itself, around the unifying concept of spline-processing. This ensures coherence in the way we model data and yields good performance. We have tested our approach in a variety of experimental conditions and report excellent results. We claim an accuracy of about a hundredth of a pixel under ideal conditions. We are also robust since the accuracy is still about a tenth of a pixel under very noisy conditions. In addition, a blind evaluation of our results compares very favorably to the work of several other researchers.

Holographic data storage

Abstract: We present an overview of our research effort on volume holographic digital data storage. Innovations, developments, and new insights gained in the design and operation of working storage platforms, novel optical components and techniques, data coding and signal processing algorithms, systems tradeoffs, materials testing and tradeoffs, and photon-gated storage materials are summarized.

Selectivity of potentiometric ion sensors.

Abstract: Selectivities of solvent polymeric membrane ion-selective electrodes (ISEs) are quantitatively related to equilibria at the interface between the sample and the electrode membrane. However, only correctly determined selectivity coefficients allow accurate predictions of ISE responses to real-world samples. Moreover, they are also required for the optimization of ionophore structures and membrane compositions. Best suited for such purposes are potentiometric selectivity coefficients as defined already in the 1960s. This paper briefly reviews the basic relationships and focuses on possible biases in the determination of selectivity coefficients. The traditional methods to determine selectivity coefficients (separate solution method, fixed interference method) are still the same as those originally proposed by IUPAC in 1976. However, several precautions are needed to obtain meaningful data. For example, errors arise when the response to a weakly interfering ion is also influenced by the primary ion leaching from the membrane. Wrong selectivity coefficients may be also obtained when the interfering agent is highly preferred and the electrode shows counterion interference. Recent advances show how such pitfalls can be avoided. A detailed recipe to determine correct potentiometric selectivity coefficients unaffected by such biases is presented.

The Determinants of Credit Spread Changes

Abstract: Using straight industrial bonds with quoted prices, we investigate the determinants of credit spread changes. We find the variables that should in theory determine credit spread changes in fact have limited explanatory power. Further, the residuals from this first-pass regression are highly cross-correlated, and principal components analysis strongly suggests they are driven by a single common factor. We investigate several macro-economic and financial variables as candidate proxies for this factor. We cannot, however, find any set of variables which explain this common systematic factor. Our results suggest the corporate bond market is a segmented market driven by corporate bond specific supply/demand shocks.

Nature of Photovoltaic Action in Dye-Sensitized Solar Cells

Abstract: We explain the cause for the photocurrent and photovoltage in nanocrystalline, mesoporous dye-sensitized solar cells, in terms of the separation, recombination, and transport of electronic charge as well as in terms of electron energetics. On the basis of available experimental data, we confirm that the basic cause for the photovoltage is the change in the electron concentration in the nanocrystalline electron conductor that results from photoinduced charge injection from the dye. The maximum photovoltage is given by the difference in electron energies between the redox level and the bottom of the electron conductor's conduction band, rather than by any difference in electrical potential in the cell, in the dark. Charge separation occurs because of the energetic and entropic driving forces that exist at the dye/electron conductor interface, with charge transport aided by such driving forces at the electron conductor/contact interface. The mesoporosity and nanocrystallinity of the semiconductor are importa...

Perspectives for dye‐sensitized nanocrystalline solar cells

Abstract: Reference LPI-ARTICLE-2000-012View record in Web of Science Record created on 2006-02-21, modified on 2017-05-12

Information-theoretic key agreement: from weak to strong secrecy for free

Abstract: One of the basic problems in cryptography is the generation of a common secret key between two parties, for instance in order to communicate privately. In this paper we consider information-theoretically secure key agreement. Wyner and subsequently Csiszar and Korner described and analyzed settings for secret-key agreement based on noisy communication channels. Maurer as well as Ahlswede and Csiszar generalized these models to a scenario based on correlated randomness and public discussion. In all these settings, the secrecy capacity and the secret-key rate, respectively, have been defined as the maximal achievable rates at which a highly-secret key can be generated by the legitimate partners. However, the privacy requirements were too weak in all these definitions, requiring only the ratio between the adversary's information and the length of the key to be negligible, but hence tolerating her to obtain a possibly substantial amount of information about the resulting key in an absolute sense. We give natural stronger definitions of secrecy capacity and secret-key rate, requiring that the adversary obtains virtually no information about the entire key. We show that not only secret-key agreement satisfying the strong secrecy condition is possible, but even that the achievable key-generation rates are equal to the previous weak notions of secrecy capacity and secret-key rate. Hence the unsatisfactory old definitions can be completely replaced by the new ones. We prove these results by a generic reduction of strong to weak key agreement. The reduction makes use of extractors, which allow to keep the required amount of communication negligible as compared to the length of the resulting key.

Enforcing service availability in mobile ad-hoc WANs

Abstract: In this paper, we address the problem of service availability in mobile ad-hoc WANs. We present a secure mechanism to stimulate end users to keep their devices turned on, to refrain from overloading the network, and to thwart tampering aimed at converting the device into a "selfish" one. Our solution is based on the application of a tamper resistant security module in each device and cryptographic protection of messages.

Processes governing phosphorus availability in temperate soils

Abstract: Phosphorus losses from agricultural soil to water bodies are mainly related to the excessive accumulation of available P in soil as a result of long-term inputs of fertilizer P. Since P is a nonrenewable resource, there is a need to develop agricultural systems based on maximum P use efficiency with minimal adverse environmental impacts. This requires detailed understanding of the processes that govern the availability of P in soil, and this paper reviews recent advances in this field. The first part of the review is dedicated to the understanding of processes governing inorganic P release from the solid phase to the soil solution and its measurement using two dynamic approaches: isotope exchange kinetics and desorption of inorganic P with an infinite sink. The second part deals with biologically driven processes. Improved understanding of the abiotic and biotic processes involved in P cycling and availability will be useful in the development of effective strategies to reduce P losses from agricultural soils, which will include matching crop needs with soil P release and the development of appropriate remediation techniques to reduce P availability in high P status soils.

Potential for increasing the content and bioavailability of Fe, Zn and Ca in plants for human nutrition.

Abstract: This paper reviews the possibility and limits for increasing the content and bioavailability of iron (Fe), zinc (Zn) and calcium (Ca) in edible parts of staple crops, such as cereals, pulses, roots and tubers as a way to combat mineral deficiencies in human populations. Theoretically, this could be achieved by increasing the total level of Fe, Zn and Ca in the plant foods, while at the same time increasing the concentration of compounds which promote their uptake (ascorbic acid), and/or by decreasing the concentration of compounds which inhibit their absorption (phytic acid or phenolic compounds). The content of Zn and Ca in grains and fruits can in some cases be increased through soil and/or foliar applications of Zn and Ca fertilisers. Plant breeding and genetic engineering techniques, however, have the greatest potential to increase Fe and Zn content in grains, roots and tubers. The possibility of enhancing Ca and ascorbic acid content in plant foods by plant breeding and genetic engineering remained to be explored. The critical factor is to ensure that the extra minerals have an adequate bioavailability for man. Given the important role of phytic acid and polyphenols in plant physiology, reducing the levels of these compounds in the edible parts of plants does not appear to be wise although introduction of phytases which are active during digestion is an exciting possibility.

Evolutionary dynamics of pathogen resistance and tolerance

Abstract: Host organisms can respond to the threat of disease either through resistance defenses (which inhibit or limit infection) or through tolerance strategies (which do not limit infection, but reduce or offset its fitness conse- quences). Here we show that resistance and tolerance can have fundamentally different evolutionary outcomes, even when they have equivalent short-term benefit for the host. As a gene conferring disease resistance spreads through a population, the incidence of infection declines, reducing the fitness advantage of carrying the resistance gene. Thus genes conferring complete resistance cannot become fixed (i.e., universal) by selection in a host population, and diseases cannot be eliminated solely by natural selection for host resistance. By contrast, as a gene conferring disease tolerance spreads through a population, disease incidence rises, increasing the evolutionary advantage of carrying the tolerance gene. Therefore, any tolerance gene that can invade a host population will tend to be driven to fixation by selection. As predicted, field studies of diverse plant species infected by rust fungi confirm that resistance traits tend to be polymorphic and tolerance traits tend to be fixed. These observations suggest a new mechanism for the evolution of mutualism from parasitism, and they help to explain the ubiquity of disease.

Orientation tuning of input conductance, excitation, and inhibition in cat primary visual cortex.

Abstract: The input conductance of cells in the cat primary visual cortex (V1) has been shown recently to grow substantially during visual stimulation. Because increasing conductance can have a divisive effect on the synaptic input, theoretical proposals have ascribed to it specific functions. According to the veto model, conductance increases would serve to sharpen orientation tuning by increasing most at off-optimal orientations. According to the normalization model, conductance increases would control the cell's gain, by being independent of stimulus orientation and by growing with stimulus contrast. We set out to test these proposals and to determine the visual properties and possible synaptic origin of the conductance increases. We recorded the membrane potential of cat V1 cells while injecting steady currents and presenting drifting grating patterns of varying contrast and orientation. Input conductance grew with stimulus contrast by 20-300%, generally more in simple cells (40-300%) than in complex cells (20-120%), and in simple cells was strongly modulated in time. Conductance was invariably maximal for stimuli of the preferred orientation. Thus conductance changes contribute to a gain control mechanism, but the strength of this gain control does not depend uniquely on contrast. By assuming that the conductance changes are entirely synaptic, we further derived the excitatory and inhibitory synaptic conductances underlying the visual responses. In simple cells, these conductances were often arranged in push-pull: excitation increased when inhibition decreased and vice versa. Excitation and inhibition had similar preferred orientations and did not appear to differ in tuning width, suggesting that the intracortical synaptic inputs to simple cells of cat V1 originate from cells with similar orientation tuning. This finding is at odds with models where orientation tuning in simple cells is achieved by inhibition at off-optimal orientations or sharpened by inhibition that is more broadly tuned than excitation.

Computational vascular fluid dynamics: problems, models and methods

Abstract: In the cardiovascular system, morphology and functionality are closely related. Altered flow conditions play an important role in the development of arterial disease. In turn, these flow conditions are modified by arterial wall changes. A detailed understanding of the local hemodynamic environment, the influence of wall modifications on flow patterns and the long-term adaptations of the vascular wall after surgical procedures can have useful clinical applications, especially in view of reconstruction and revascularization operations. Some of these alterations are not well-understood, making it quite difficult to foresee short- and long-term evolution of the atherosclerotic disease and to plan an aggressive approach. Computational fluid dynamics (CFD) allows the carrying out of simulations at low cost and in completely controlled conditions. Three different issues are relevant to this subject and are addressed in this paper: (1) the definition of suitable mathematical models; (2) pre-processing of clinical data; and (3) the development of appropriate numerical techniques

Hydration Properties of Dietary Fibre and Resistant Starch: a European Collaborative Study

Abstract: Hydration properties related to the fibre matrix have been defined and measured in 19 European laboratories using common methods and the substrates: resistant starches, pea hull, citrus pulp and apple pulp. Swelling and water retention capacity (WRC) were the major hydration properties studied. The objective was to derive standardized methods with an included estimate of experimental variation or statistical tolerance. Measurement of swelling and WRC were complemented by measurement of water absorption and porosity. Swelling (∼7 mL/g) and WRC (∼4 g/g) were relatively low for resistant starches and pea hull. Swelling and WRC of citrus (∼11 mL/g: ∼11 g/g) and apple pulp (∼7 mL/g: ∼5g/g) were lower than expected, probably reflecting effects of processing on matrix structure. Coefficient of variation was higher between laboratories than within laboratory. The relationship between swelling, WRC, absorption and porosity, as well as matrix effects, are discussed.

Population Dynamics of Spiking Neurons: Fast Transients, Asynchronous States, and Locking

Abstract: An integral equation describing the time evolution of the population activity in a homogeneous pool of spiking neurons of the integrate-and-fire type is discussed. It is analytically shown that transients from a state of incoherent firing can be immediate. The stability of incoherent firing is analyzed in terms of the noise level and transmission delay, and a bifurcation diagram is derived. The response of a population of noisy integrate-and-fire neurons to an input current of small amplitude is calculated and characterized by a linear filter L. The stability of perfectly synchronized "locked" solutions is analyzed.

Phase transitions and strain-induced ferroelectricity in SrTiO 3 epitaxial thin films

Abstract: A Landau-Ginsburg-Devonshire-type theory is used to describe the mechanical substrate effect on equilibrium states and phase transitions in SrTiO3 epitaxial thin films. The misfit strain-temperature phase diagram of SrTiO3 films is developed taking into account the existence of two coupled instabilities (antiferrodistortive and ferroelectric) in this crystal. It is shown that SrTiO3 films remain paraelectric down to 0 K only in a narrow range of small negative misfit strains between -2 x 10(-3) and -2.2 x 10(-4). Outside this "paraelectric gap," he 2D clamping and straining of the film by the substrate leads to the appearance of ferroelectricity in SrTiO3 films. The temperature of the ferroelectric transition increases rapidly outside the aforementioned misfit strain range.

On coding for block fading channels

Abstract: This work considers the achievable performance for coded systems adapted to a multipath block-fading channel model. This is a particularly useful model for analyzing mobile-radio systems which employ techniques such as slow frequency-hopping under stringent time-delay or bandwidth constraints for slowly time-varying channels. In such systems, coded information is transmitted over a small number of fading channels in order to achieve diversity. Bounds on the achievable performance due to coding are derived using information-theoretic techniques. It is shown that high diversity can be achieved using relatively simple codes as long as very high spectral efficiency is not required. Examples of simple block codes and carefully chosen trellis codes are given which yield, in some cases, performances approaching the information-theoretic bounds.

Midpoints versus endpoints: The sacrifices and benefits

Abstract: On May 25–26, 2000 in Brighton (England), the third in a series of international workshops was held under the umbrella of UNEP addressing issues in Life Cycle Impact Assessment (LCIA). The workshop provided a forum for experts to discuss midpoint vs. endpoint modeling. Midpoints are considered to be links in the cause-effect chain (environmental mechanism) of an impact category, prior to the endpoints, at which characterization factors or indicators can be derived to reflect the relative importance of emissions or extractions. Common examples of midpoint characterization factors include ozone depletion potentials, global warming potentials, and photochemical ozone (smog) creation potentials. Recently, however, some methodologies have adopted characterization factors at an endpoint level in the cause-effect chain for all categories of impact (e.g., human health impacts in terms of disability adjusted life years for carcinogenicity, climate change, ozone depletion, photochemical ozone creation; or impacts in terms of changes in biodiversity, etc.). The topics addressed at this workshop included the implications of midpoint versus endpoint indicators with respect to uncertainty (parameter, model and scenario), transparency and the ability to subsequently resolve trade-offs across impact categories using weighting techniques. The workshop closed with a consensus that both midpoint and endpoint methodologies provide useful information to the decision maker, prompting the call for tools that include both in a consistent framework.

Analysis and direct numerical simulation of the flow at a gravity-current head. Part 1. Flow topology and front speed for slip and no-slip boundaries

Abstract: Direct numerical simulations are performed of gravity-current fronts in the lock-exchange configuration The case of small density differences is considered, where the Boussinesq approximations can be adopted The key objective of the investigation is a detailed analysis of the flow structure at the foremost part of the front, where no previous high-resolution data were available For the simulations, high-order numerical methods are used, based on spectral and spectral-element discretizations and compact finite differences A three-dimensional simulation is conducted of a front spreading along a no-slip boundary at a Reynolds number of about 750 The simulation exhibits all features typically observed in experimental flows near the gravity-current head, including the lobe-and-cleft structure at the leading edge The results reveal that the flow topology at the head differs from what has been assumed previously, in that the foremost point is not a stagnation point in a translating system Rather, the stagnation point is located below and slightly behind the foremost point in the vicinity of the wall The relevance of this finding for the mechanism behind the lobe-and-cleft instability is discussed In order to explore the high-Reynolds-number regime, and to assess potential Reynolds-number effects, two-dimensional simulations are conducted for Reynolds numbers up to about 30 000, for both no-slip and slip (ie shear-stress free) boundaries It is shown that although quantitative Reynolds-number effects persist over the whole range examined, no qualitative changes in the flow structure at the head can be observed A comparison of the two-dimensional results with laboratory data and the three-dimensional simulation provides evidence that a two-dimensional model is able to capture essential features of the flow at the head The simulations also show that for the free-slip case the shape of the head agrees closely with the classical inviscid theory of Benjamin