École Polytechnique Fédérale de Lausanne

About: École Polytechnique Fédérale de Lausanne is a facility organization based out in Lausanne, Switzerland. It is known for research contribution in the topics: Population & Catalysis. The organization has 44041 authors who have published 98296 publications receiving 4372092 citations. The organization is also known as: EPFL & ETHL.

Designer matrices for intestinal stem cell and organoid culture

Abstract: Epithelial organoids recapitulate multiple aspects of real organs, making them promising models of organ development, function and disease. However, the full potential of organoids in research and therapy has remained unrealized, owing to the poorly defined animal-derived matrices in which they are grown. Here we used modular synthetic hydrogel networks to define the key extracellular matrix (ECM) parameters that govern intestinal stem cell (ISC) expansion and organoid formation, and show that separate stages of the process require different mechanical environments and ECM components. In particular, fibronectin-based adhesion was sufficient for ISC survival and proliferation. High matrix stiffness significantly enhanced ISC expansion through a yes-associated protein 1 (YAP)-dependent mechanism. ISC differentiation and organoid formation, on the other hand, required a soft matrix and laminin-based adhesion. We used these insights to build a fully defined culture system for the expansion of mouse and human ISCs. We also produced mechanically dynamic matrices that were initially optimal for ISC expansion and subsequently permissive to differentiation and intestinal organoid formation, thus creating well-defined alternatives to animal-derived matrices for the culture of mouse and human stem-cell-derived organoids. Our approach overcomes multiple limitations of current organoid cultures and greatly expands their applicability in basic and clinical research. The principles presented here can be extended to identify designer matrices that are optimal for long-term culture of other types of stem cells and organoids.

Finite-length analysis of low-density parity-check codes on the binary erasure channel

Abstract: In this paper, we are concerned with the finite-length analysis of low-density parity-check (LDPC) codes when used over the binary erasure channel (BEC). The main result is an expression for the exact average bit and block erasure probability for a given regular ensemble of LDPC codes when decoded iteratively. We also give expressions for upper bounds on the average bit and block erasure probability for regular LDPC ensembles and the standard random ensemble under maximum-likelihood (ML) decoding. Finally, we present what we consider to be the most important open problems in this area.

A tutorial on adaptive MCMC

Abstract: We review adaptive Markov chain Monte Carlo algorithms (MCMC) as a mean to optimise their performance Using simple toy examples we review their theoretical underpinnings, and in particular show why adaptive MCMC algorithms might fail when some fundamental properties are not satisfied This leads to guidelines concerning the design of correct algorithms We then review criteria and the useful framework of stochastic approximation, which allows one to systematically optimise generally used criteria, but also analyse the properties of adaptive MCMC algorithms We then propose a series of novel adaptive algorithms which prove to be robust and reliable in practice These algorithms are applied to artificial and high dimensional scenarios, but also to the classic mine disaster dataset inference problem

Science, technology and the future of small autonomous drones

Abstract: We are witnessing the advent of a new era of robots - drones - that can autonomously fly in natural and man-made environments. These robots, often associated with defence applications, could have a major impact on civilian tasks, including transportation, communication, agriculture, disaster mitigation and environment preservation. Autonomous flight in confined spaces presents great scientific and technical challenges owing to the energetic cost of staying airborne and to the perceptual intelligence required to negotiate complex environments. We identify scientific and technological advances that are expected to translate, within appropriate regulatory frameworks, into pervasive use of autonomous drones for civilian applications.

Benefiting from the Unique Properties of Lanthanide Ions

Abstract: The recent upsurge of interest in contrast agents for magnetic resonance imaging, of luminescent chemosensors for medical diagnostic, and lately, for optical imaging of cells has generated an impressive momentum for the coordination and supramolecular chemistry of trivalent lanthanide ions. We shortly review the synthetic methods allowing the introduction of these spherical ions with fascinating optical and magnetic properties into elaborate mono- and polymetallic edifices. We then illustrate these methods by selected examples describing the use of (i) a coronand to produce luminescent liquid crystals, (ii) derivatized calixarenes for 4f−5f element separation, (iii) podates for the production of nanoparticles with high relaxivity and for sensitizing the near-infrared (NIR) emission, and (iv) self-assembly processes for producing functional bimetallic edifices.