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Institution

Edith Cowan University

EducationPerth, Western Australia, Australia
About: Edith Cowan University is a education organization based out in Perth, Western Australia, Australia. It is known for research contribution in the topics: Population & Tourism. The organization has 4040 authors who have published 13529 publications receiving 339582 citations. The organization is also known as: Edith Cowan & ECU.


Papers
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Journal ArticleDOI
10 May 2018-PLOS ONE
TL;DR: This study presents a number of experiments on various steps of quantitative PCR workflow, and demonstrates how to perform a quantitative PCR experiment with human skeletal muscle samples in an exercise study, and tests some common mistakes in performing qPCR.
Abstract: Gene expression analysis by quantitative PCR in skeletal muscle is routine in exercise studies. The reproducibility and reliability of the data fundamentally depend on how the experiments are performed and interpreted. Despite the popularity of the assay, there is a considerable variation in experimental protocols and data analyses from different laboratories, and there is a lack of consistency of proper quality control steps throughout the assay. In this study, we present a number of experiments on various steps of quantitative PCR workflow, and demonstrate how to perform a quantitative PCR experiment with human skeletal muscle samples in an exercise study. We also tested some common mistakes in performing qPCR. Interestingly, we found that mishandling of muscle for a short time span (10 mins) before RNA extraction did not affect RNA quality, and isolated total RNA was preserved for up to one week at room temperature. Demonstrated by our data, use of unstable reference genes lead to substantial differences in the final results. Alternatively, cDNA content can be used for data normalisation; however, complete removal of RNA from cDNA samples is essential for obtaining accurate cDNA content.

115 citations

Journal ArticleDOI
TL;DR: A review of the published datasets on CO2 wettability of geological formations establishes a repository of the recent contact angle data, which thus assists to enhance the current understanding of the subject.

115 citations

Journal ArticleDOI
TL;DR: In this paper, anode graphite was recovered from a spent lithium ion battery (LIB) and reutilized as a carbon precursor to obtain graphene-based materials, which demonstrated excellent catalytic ozonation activity against organic pollutants removal.
Abstract: Anode graphite was recovered from a spent lithium ion battery (LIB) and reutilized as a carbon precursor to obtain graphene-based materials. Characterization results revealed that impurities were removed from the obtained graphite powder by cleansing processes. The as-synthesized reduced graphene oxide (rGO) from the purified graphite (LIB-rGO) demonstrated excellent catalytic ozonation activity against organic pollutants removal. To probe potential catalytic active sites, LIB-rGOs with different defective levels but similar oxygen contents were synthesized. Catalytic ozonation tests revealed that a higher defective level resulted in a greater catalytic activity. Density functional theory (DFT) calculation further demonstrated that ozone molecules could spontaneously decompose into active oxygen species on graphene structural vacancies and edges, which consolidated the role of defective structure in catalytic ozonation activity. Meanwhile, we discovered the pollutant-structure-dependent behavior of dominant reactive oxygen species (ROS) with the aid of radical scavenging tests and electron paramagnetic resonance (EPR) spectra. For phenolic pollutants vulnerable to direct ozone attacking, superoxide radicals (O2 −) and singlet oxygen (1O2) were found to be responsible ROS, whereas hydroxyl radicals ( OH) were identified as the principle ROS for aliphatic organic pollutants destruction. This study not only put forward a possible way for reutilization of waste LIB anode, but also stepped further for investigating the catalytic ozonation mechanism towards the graphene-based materials including the active sites and the generation of ROS.

115 citations

Journal ArticleDOI
TL;DR: In this article, the authors investigated the temporal association between feeling connected to school and mental health prior to and over the transition period and found that increased depression and anxiety is associated with decreased connectedness to school.
Abstract: During the transition from primary to secondary school, students typically experience a new social environment, moving from primary school with small intact classes throughout the day with one main teacher, to a larger secondary school with teachers, classrooms and often classmates changing throughout the day. During this time, students report a reduced sense of connectedness, which has been associated with symptoms of depression and anxiety. This study investigated the temporal association between feeling connected to school and mental health prior to and over the transition period. Data were obtained from 3,459 students in a longitudinal study of adolescents' knowledge, attitudes and experiences of bullying victimisation and perpetration during the transition from primary school to secondary school. Students completed a questionnaire at four time points from Grade 7 to the end of Grade 9. Path analysis was used to model relationships between school connectedness, depression and anxiety. The findings suggest reciprocal relationships between connectedness and mental health where increased connectedness to school is associated with decreased depression and anxiety; conversely, increased depression and anxiety is associated with decreased connectedness to school. The significant reciprocal associations found in the cross-lag models in the first two years of secondary school indicate the need to intervene during the transition period to improve students' social and mental health outcomes.

115 citations

Journal ArticleDOI
TL;DR: In this article, the effects of a 17-year long-term fertilization on soil microbial biomass C (SMBC and N), soluble organic C, and soluble organic N during the maize growing season were evaluated in a loess soil (Eum-Orthic Anthrosol) in northwest China.
Abstract: As labile organic pools, soluble organic matter and soil microbial biomass are sensitive to changes in soil management and therefore good indicators of soil quality. Effects of a 17-year long-term fertilization on soil microbial biomass C (SMBC) and N (SMBN), soluble organic C, and soluble organic N during the maize growing season were evaluated in a loess soil (Eum-Orthic Anthrosol) in northwest China. The fertilization treatments included no fertilizer (CK), inorganic N, P, and K fertilizer (NPK), cattle manure plus NPK fertilizer (MNPK), and straw plus NPK fertilizer (SNPK). Our results showed that C storage in the 0–20 cm soil layer was 28% to 81% higher in the fertilized treatments compared to the unfertilized treatment. In the 0–10 cm soil layer, SMBC and SMBN in the three fertilized treatments were higher than in the unfertilized treatment on all sampling dates, while microbial biomass C and N in the 0−10 cm soil layers were the highest at grain filling. In the same soil layer, soil-soluble organic C generally decreased in the order MNPK > SNPK > NPK > CK, while soluble organic N was the highest in the MNPK followed by the SNPK treatment. There was no significant difference in soluble organic N in the NPK and CK treatments throughout most of the maize growing season. Changes in soluble organic N occurred along the growing season and were more significant than those for soluble organic C. Soluble organic N was the highest at grain filling and the lowest at harvest. Overall, our results indicated that microbial biomass and soluble organic N in the surface soil were generally the highest at grain filling when maize growth was most vigorous. Significant positive relationships were found between soluble organic C and SMBC and between soluble organic N and SMBN.

115 citations


Authors

Showing all 4128 results

NameH-indexPapersCitations
Paul Jackson141137293464
William J. Kraemer12375554774
D. Allan Butterfield11550443528
Kerry S. Courneya11260849504
Robert U. Newton10975342527
Roger A. Barker10162039728
Ralph N. Martins9563035394
Wei Wang95354459660
David W. Dunstan9140337901
Peter E.D. Love9054624815
Andrew Jones8369528290
Hongqi Sun8126520354
Leon Flicker7946522669
Mark A. Jenkins7947221100
Josep M. Gasol7731322638
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Performance
Metrics
No. of papers from the Institution in previous years
YearPapers
202350
2022156
20211,433
20201,372
20191,213
20181,023