Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto

About: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto is a based out in . It is known for research contribution in the topics: Population & Genus. The organization has 2143 authors who have published 3674 publications receiving 71071 citations. The organization is also known as: FFCLRP & FFCLRP-USP.

Transcriptome Analysis of Aspergillus nidulans Exposed to Camptothecin-Induced DNA Damage

Abstract: uvsC RAD51 (the homologue of the RAD51 gene), and cshA (encoding a homologue of the excision repair protein ERCC-6 [Cockayne’s syndrome protein]). The induced transcript levels of these genes in the presence of CPT require uvsB ATR . These genes were deleted, and surprisingly, only the uvsC mutant strain was sensitive to CPT; however, the others displayed sensitivity to a range of DNA-damaging and oxidative stress agents. These results indicate that the selected genes when inactivated display very complex and heterogeneous sensitivity behavior during growth in the presence of agents that directly or indirectly cause DNA damage. Moreover, with the exception of UvsC, deletion of each of these genes partially suppressed the sensitivity of the uvsB strain to menadione and paraquat. Our results provide the first insight into the overall complexity of the response to DNA damage in filamentous fungi and suggest that multiple pathways may act in parallel to mediate DNA repair.

The inducing NO-vasodilation by chemical reduction of coordinated nitrite ion in cis-[Ru(NO2)L(bpy)2]+ complex

Abstract: The synthesis of [Ru(NO2)L(bpy)2]+ (bpy = 2,2′-bipyridine and L = pyridine (py) and pyrazine (pz)) can be accomplished by addition of [Ru(NO)L(bpy)2](PF6)3 to aqueous solutions of physiological pH. The electrochemical processes of [Ru(NO2)L(bpy)2]+ in aqueous solution were studied by cyclic voltammetry and differential pulse voltammetry. The anodic scan shows a peak around 1.00 V vs. Ag/AgCl attributed to the oxidation process centered on the metal ion. However, in the cathodic scan a second peak around −0.60 V vs. Ag/AgCl was observed and attributed to the reduction process centered on the nitrite ligand. The controlled reduction potential electrolysis at −0.80 V vs. Ag/AgCl shows NO release characteristics as judged by NO measurement with a NO-sensor. This assumption was confirmed by ESI/MS+ and spectroelectrochemical experiment where cis-[Ru(bpy)2L(H2O)]2+ was obtained as a product of the reduction of cis-[RuII(NO2)L(bpy)2]+. The vasorelaxation observed in denuded aortic rings pre-contracted with 0.1 μmol L−1 phenylephrine responded with relaxation in the presence of cis-[RuII(NO2)L(bpy)2]+. The potential of rat aorta cells to metabolize cis-[RuII(NO2)L(bpy)2]+ was also followed by confocal analysis. The obtained results suggest that NO release happens by reduction of cis-[RuII(NO2)L(bpy)2]+ inside the cell. The maximum vasorelaxation was achieved with 1 × 10−5 mol L−1 of cis-[RuII(NO2)L(bpy)2]+ complex.

Protection against Paracoccidioides brasiliensis infection conferred by the prophylactic administration of native and recombinant ArtinM

Abstract: We determined the prophylactic effect of both the d-mannose-binding lectin ArtinM extracted from the seeds of Artocarpus integrifolia (jackfruit) and its recombinant counterpart during the course o...