Fifth Affiliated Hospital of Xinjiang Medical University

About: Fifth Affiliated Hospital of Xinjiang Medical University is a healthcare organization based out in Ürümqi, China. It is known for research contribution in the topics: Apoptosis & Population. The organization has 241 authors who have published 139 publications receiving 1190 citations.

The complete mitochondrial genome sequence of Hemibagrus sp. (Siluriformes: Bagridae), molecular data for species identification

Abstract: In this study, we determined and described the complete mitogenome sequence of Hemibagrus sp. for the first time, which is 16,513 bp in length, and contains 37 genes, including 13 protein-coding genes, 2 rRNAs, 22 tRNAs, 1 origin of replication on the light-strand (OL) and a putative control region. The overall base composition was 31.1% A, 26.9% T, 26.9% C, 15.1% G, with a slight AT bias (58.0%). All protein-coding genes shared the start codon ATG, except for COI, which began with GTG. The tRNA-SerUGC couldn’t be folded into the typical cloverleaf secondary structure because its dihydrouridine arm is replaced by a simple loop. These results are expected to provide useful molecular data for species identification and further phylogenetic studies of Bagridae and Siluriformes.

Application of digital navigation system in treatment of adult DDH surgery

Abstract: Objective To create a digital acetabulum molding navigation path and investigate application value of digital navigation technology in total hip replacement for developmental dysplasia of the hip. Methods Ten adult patients with DDH(Crowe Ⅳ) were divided into computer navigation group and non-computer navigation group. In the computer navigation group of 5 cases, CAD, reverse engineering software, computer-aided design and 3D print were used for DDH acetabulum molding preoperative and intraoperative localization and simulation of operation on the computer workstation. The surgery path planning, 3D printing solid model preoperative drills, computer-aided design navigation template assisted in total hip arthroplasty. In the non-computer navigation group, the conventional techniques were used in total hip replacement. Results In navigation group actual operation and computer simulation of operation image and 3D rapid prototyping of joint model were very similar. Between navigation and non-navigation group there were significant differences in surgery time(t =-5.284, P =0.024), blood loss(t =-1.732, P =0.034) abduction angle offset amplitude(t =-3.734, P =0.024). Ten cases were followed up for average 9.5(3-17)months. The femoral fracture occurred in one case of non-navigation group when the prosthesis was implanted, and was fixed with plate in surgery. Navigation group had high accuracy with satisfactory prosthesis position.Conclusion In unilateral congenital hip dysplasia acetabular location, total hip arthroplasty with computer-aided design navigation template can accurately reconstruct the affected hip rotation center, and effectively improve the accuracy of the prosthesis placement with advantages of higher accuracy and quicker positioning.

[Effects of bone marrow mesenchymal stem cell transplantation on the expression of stromal cell-derived factor-1α and vascular endothelial growth factor in rats with acute hepatic failure].

Abstract: Objective: To investigate the curative effect of bone marrow mesenchymal stem cells (BMSCs) transplantation on the expression of stromal cell-derived growth factor (SDF-1 α) and vascular endothelial growth factor (VEGF) in rats with acute hepatic failure, and to compare the effects of two transplantation pathways. Methods: Eighty-four rats with acute liver failure (ALF) induced by D-galactosamine combined with lipopolysaccharide were randomly divided into control group, tail vein and portal vein transplantation group. The latter two groups were injected allogenic BMSCs into the tail vein and portal vein. Blood samples and liver tissue samples were collected at 24, 72, 120, and 168h after transplantation to detect serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST). The improvement of liver function before and after BMSCs transplantation was compared. The expression of VEGF and SDF-1a in liver tissue was detected by immunofluorescence and Western blot. Data measurement between two groups was performed by analysis of variance and the correlation analysis was performed by Spearman's rank correlation coefficient. Results: Serum ALT and AST levels in the tail vein and portal vein transplantation group peaked at 24 h after transplantation, which were (134.60 ± 58.08 IU/L), (179.20 ± 86.68 IU/L), and (131.00 ± 54.47 IU/L), (173.50 ± 93.10 IU/L). In addition, 168h after transplantation it decreased to (46.10 ± 8.40 IU/L), (95.67 ± 13.80 IU/L) and (19.30 ± 1.30 IU/L), (54.30 ± 6.00 IU/L). After 120 and 168 hours of BMSCs transplantation, the levels of serum ALT and AST in tail vein and portal vein transplantation group were significantly higher than control group (F ≥ 12.51, P 0.05). Correlation analysis showed that the expression levels of SDF-1α and VEGF in liver tissues were positively correlated (r = 0.923, P < 0.05). Conclusion: BMSCs transplantation can promote the secretion of VEGF for recovery of liver function to reduce the degree of inflammation and necrosis in rats with ALF.

Inhibitory effects of candesartan on KCa3.1 potassium channel expression and cell culture and proliferation in peripheral blood CD4+T lymphocytes in Kazakh patients with hypertension from the Xinjiang region.

Abstract: BACKGROUND AND AIM Increasing evidence confirms that potassium channels are essential for lymphocyte activation, suggesting an involvement in the development of hypertension. Moreover, chronic inflammation is regarded as a direct or indirect manifestation of hypertension, highlighting the theoretical mechanisms. In this study, we investigated changes in KCa3.1 potassium channel expression in the blood of hypertensive and healthy Kazakh people in north-west China. METHODS Flow cytometry technology was used for T-lymphocyte subtype analysis. Changes in the messenger RNA and protein expression of the KCa3.1 potassium channel in CD4+ T lymphocytes were detected using real-time quantitative polymerase chain reaction and western blots, using CD4+ T-cell samples from hypertensive Kazakh patients divided into candesartan and TRAM-34 treatment groups, and healthy case controls. Peripheral blood CD4+ T lymphocytes were activated and proliferated in vitro and then incubated for 0, 24, and 48 h under various treatment conditions. Changes in CD4+ T-lymphocytic proliferation were determined using Cell Counting Kit-8 and electron microscope photography. RESULTS Expression of KCa3.1 was significantly higher in the hypertensive patients than in the controls (p < 0.05). Compared with the healthy group, Kazakh hypertensive patients had a reduced proportion of CD4+ T lymphocytes (p < 0.05).Candesartan and TRAM-34 intervention for 24 h and 48 h inhibited the expression of Kv1.3 and KCa3.1 at mRNA and protein level (p < 0.05). CONCLUSIONS Increase in functional KCa3.1 channels expressed in CD4+ T lymphocytes of Kazakh patients with hypertension was blocked by candesartan, providing theoretical support for hypertension treatment at the cellular ion channel level. Candesartan may potentially regulate hypertensive inflammatory responses by inhibiting T-lymphocytic proliferation and KCa3.1 potassium channel expression in CD4 + T lymphocytes.

Roles of substance P and transient receptor potential vanilloid 1 in neuralgia in rats with chronic nonbacterial prostatitis

Abstract: OBJECTIVE To study the possible mechanisms of chronic nonbacterial prostatitis (CNP) pain. METHODS CNP models were established in male Wistar rats by the autoimmune method. Then the paw withdrawal threshold (PWT) was detected using the Von Frey filament, prostate pathological examination was conducted, the expressions of substance P (SP) and transient receptor potential vanilloid 1 (TRPV1) in the prostate tissue and L5-S2 spinal segments were determined by immunohistochemistry and their correlations were analyzed. RESULTS Compared with the control group, the CNP model rats showed markedly decreased PWT (P < 0.05) and obvious inflammation in the prostate tissue, with significant differences in the scope of lesion and interstitial lymphocyte infiltration (P < 0.05). The expressions of SP and TRPV1 in the prostate and spinal cord dorsal horn L5-S2 were remarkably upregulated in the models as compared with the control rats (P < 0.05). However, the expression of SP in the prostate was not correlated with that in the spinal cord (r = 0.099, P = 0.338), nor was that of TRPV1 (r = 0.000, P = 0.5). CONCLUSION SP and TRPV1 were involved in the formation and persistence of pain in CNP rats through their upregulated expressions in the L5-S2 spinal segments.