Showing papers by "Flinders University published in 1969"
145 citations
92 citations
TL;DR: A comparison of the glutamine-synthesizing activity and the gamma-glutamyltransferase activity of glutamine synthetase in Aspergillus and Neurospora gave no indication that these fungi produce different forms of glutamines when grown on ammonia or l- glutamate as nitrogen sources.
Abstract: 1. Aspergillus nidulans, Neurospora crassa and Escherichia coli were grown on media containing a range of concentrations of nitrate, or ammonia, or urea, or l-glutamate, or l-glutamine as the sole source of nitrogen and the glutamate dehydrogenate and glutamine synthetase of the cells measured. 2. Aspergillus, Neurospora and Escherichia coli cells, grown on l-glutamate or on high concentrations of ammonia or on high concentrations of urea, possessed low glutamate dehydrogenase activity compared with cells grown on other nitrogen sources. 3. Aspergillus, Neurospora and Escherichia coli cells grown on l-glutamate possessed high glutamine synthetase activity compared with cells grown on other nitrogen sources. 4. The hypothesis is proposed that in Aspergillus, Neurospora and Escherichia coli l-glutamate represses the synthesis of glutamate dehydrogenase and l-glutamine represses the synthesis of glutamine synthetase. 5. A comparison of the glutamine-synthesizing activity and the γ-glutamyltransferase activity of glutamine synthetase in Aspergillus and Neurospora gave no indication that these fungi produce different forms of glutamine synthetase when grown on ammonia or l-glutamate as nitrogen sources.
90 citations
TL;DR: The results in the regulation of tumour-cell 5'-nucleotidase activity were obtained in a soluble form after treatment of a particulate fraction from Ehrlich ascites-tumour cells with deoxycholate and the possible significance of the results is discussed.
Abstract: 1. 5′-Nucleotidase activity was obtained in a soluble form after treatment of a particulate fraction from Ehrlich ascites-tumour cells with deoxycholate. The relative rates of hydrolysis of 6-thioinosine 5′-phosphate, UMP, AMP, CMP, GMP, IMP, xanthosine monophosphate, thymidine monophosphate and 2′,3′-AMP were 180, 129, 100, 93, 83, 79, 46, 41 and 3 respectively. 2. Values found for the Michaelis constant were: AMP, 67±12μm; IMP, 111±8μm; GMP, 93μm. 3. ATP and thymidine triphosphate were competitive inhibitors of AMP hydrolysis (inhibitor constants 0·4 and 4·8μm respectively); UTP, GTP and CTP were mixed competitive and non-competitive inhibitors. Thymidine triphosphate was a competitive inhibitor of IMP hydrolysis (inhibitor constant 14·4μm) and ATP, UTP and GTP showed mixed competitive and non-competitive inhibition. 4. ATP, thymidine triphosphate, UTP, GTP and CTP did not completely inhibit hydrolysis of AMP, IMP and UMP; the concentrations of ATP required to inhibit AMP and IMP hydrolysis by 50% were 12 and 230μm respectively. 5. Non-hyperbolic curves relating activity to UMP concentration were obtained in the presence and absence of triphosphates. 6. After fractionation on Sephadex G-200 columns a single peak of 5′-nucleotidase activity (particle weight 120000–125000) was obtained with AMP, IMP and GMP as substrates. UMP hydrolysis was catalysed by enzyme in this peak and in two slower peaks corresponding to apparent particle weights of 32000 and 16000; a single component (particle weight 120000), reacting with UMP and insensitive to UTP inhibition, was obtained when the column was eluted with buffer containing 1mm-UMP. 7. The possible significance of the results in the regulation of tumour-cell 5′-nucleotidase is discussed.
63 citations
TL;DR: The influx and efflux of Cl−, K+ and Na+ have been measured in a series of experiments with comparable cells of Chara corallina, under different experimental conditions and a proposed passive component of the K+ influx was sometimes, but not always, consistent with observed changes in the potential difference and conductance of the plasmalemma.
45 citations
TL;DR: The latter made an insignificant contribution to dominant lethality in Drosophila following heliotrine treatment, and it is suggested that most dominant lethals arise as a result of intrachromosomal rearrangements with lethal effect, or to isochromatid aberrations.
Abstract: The occurrence of chromosomal damage produced by the pyrrolizidine alkaloid heliotrine in Drosophila has been demonstrated using the frequencies of induced dominant lethals, partial and complete sex-chromosome loss, translocations and sex-ratio shifts as measures of the resulting damage. It has been shown that the frequency of XO males induced by the alkaloid is insufficient to account for the observed deviation in the sex-ratio even on the unlikely assumption that all XO males result from X-chromosome loss. Therefore it is proposed that the remaining deviation is due to the differential production of dominant lethals in the X- and Y-bearing sperm. Assuming that all XO males result from X-chromosome loss, and dominant lethals are produced in a ratio of 1:0.85 for the X- and Y-bearing sperms respectively, then a close fit with the experimentally observed sex-ratios is obtained. Translocations are produced with a low frequency. Evidence is discussed which indicates that there is a close relationship between the frequencies of induced translocations and asymmetrical exchanges. On this basis it is concluded that the latter made an insignificant contribution to dominant lethality in Drosophila following heliotrine treatment. It is suggested that most dominant lethals arise as a result of intrachromosomal rearrangements with lethal effect, or to isochromatid aberrations. It is further proposed that the different sensitivities of the X-ray and Y-bearing sperms must be due to the different frequencies of lethals occurring in the X- and Y-chromosomes.
38 citations
TL;DR: Investigations into the chemical nature of spermagglutinins detected in the sera gave results which appear to conflict with earlier suggestions that they are antibodies1 or γ-globulins and, in particular, IgG3.
Abstract: THERE is uncertainty about the chemical nature of spermagglutinins detected in the sera of some men and some women. The investigations described in this article gave results which appear to conflict with earlier suggestions that they are antibodies1 or γ-globulins2 and, in particular, IgG3. Preliminary studies in this laboratory, using ammonium sulphate fractionation of several spermagglutinating sera, had shown that the 60 per cent saturated fractions possessed the greatest spermagglutinating activity, whereas the greatest concentrations of γ-globulins were in the 40 per cent fractions4.
32 citations
TL;DR: This paper found that subjects tended to display positive interest in information, preferred consistent to inconsistent information, and preferred novel to familiar information, while high intolerant and high dogmatic subjects showed a more pronounced preference for consistent as opposed to inconsistent.
Abstract: 77 male and 81 female Ss first wrote arguments favouring American intervention in South Vietnam and arguments opposing intervention. They then rated the direction and degree of their interest in reading each of 4 sets of information concerned with this issue: familiar arguments-pro intervention, familiar arguments-con intervention, novel arguments-pro intervention, novel arguments-con intervention. Subsequently they completed an attitude scale concerned with American intervention, Budner's test of intolerance of ambiguity, and Form E of Rokeach's dogmatism scale. Results showed that: (a) subjects tended to display positive interest in information, preferred consistent to inconsistent information, and preferred novel to familiar information; (b) high intolerant and high dogmatic subjects showed a more pronounced preference for consistent as opposed to inconsistent information than did low intolerant and low dogmatic subjects; (c) high intolerant and high dogmatic subjects showed a less pronounced ...
32 citations
TL;DR: In this paper, a new method for the numerical solution of the diffusion-type equation Hψ = − ∂ψ / ∂β is presented for both infinite and bounded regions.
32 citations
TL;DR: In this paper, the triton binding energy and doublet scattering length were calculated for the Hamada-Johnston potential and the D-state and S-state probabilities were calculated to be 9% and 2% respectively.
26 citations
TL;DR: In this paper, a more general method of measuring potential influence which involves the use of matrix representations of group structure is presented, which is illustrated in a study dealing with the effect of the group structure upon productivity.
Abstract: A major proposition of Fiedler's contingency model of leadership is that the effectiveness of a particular style of leadership is dependent upon the favourability of the group situation. Favourability refers to the potential influence of the leader and is measured by ordering several dimensions of group structure. This paper presents a more general method of measuring potential influence which involves the use of matrix representations of group structure. The use of this index of potential influence is illustrated in a study dealing with the effect of group structure upon productivity.
TL;DR: With minor modifications the technique has been used successfully to identify amylase isozymes in the other body fluids and tissue homogenates and to identifyAmylase variants with different electrophoretic mobilities to be identified.
TL;DR: The South Sea evangelical mission in the Solomon Islands: The foundation years as discussed by the authors was the first effort of the South Sea mission to the Solomon islands, and was based on the Bible translation.
Abstract: (1969). The South Sea evangelical mission in the Solomon Islands: The foundation years. The Journal of Pacific History: Vol. 4, No. 1, pp. 41-64.
TL;DR: In this article, deuterium exchange reactions with propane, ethane, and methane were studied over various ultra-high-vacuum-deposited nickel films.
TL;DR: The role of linear differentiation of chromosomes on the proportionality between chromosome length and DNA content and the amounts of nucleic acids in the cells of different species of higher plants are studied.
Abstract: DYER, A. r. 1964. Heterochromatin in American and Japanese species of Trillium. Cytologia, 29, 171-190. JOHN, B., AND HEWITT, G.M. 1966. Karyotype stability and DNA variability in the Acrididae. Chromosoma, 20, 165-1 72. JONES, R. NEIL, AND REES, H. 1968. Influence of B-chromosomes in rye upon the nuclear phenotype. In press. KEYL, H. 0. 1964. Verdopplung des DNA Gehalte kiciner Chromosomen Abschnitte als Faktor der Evolution. Xaturwissenschaften, 51, 46-47. KEYL, n. G. 1965. A demonstrable local and geometric increase in the chromosomal DNA of Chironomm. Experienlia, 21, 191. LIMA DE PARIA, A. 1959. Differential uptake of initiated thymidine into heteroand euchromatin in Melanopus and Secale. 3'. Biophys. Biochem. Cytology, 6, 457-466. MARTIN, P. o. 1966. Variations in the amounts of nucleic acids in the cells of different species of higher plants. Exp. Cell Res., 44, 84-94. MCLEISH, j., AND SuNDERLAND, N. 1961. Measurements of deoxyribonucleic acid (DNA) in higher plants by Feulgen photometry and chemical methods. Exp. Cell Res., 24, 527-540. MITFWOCH, U. 1968. Scope and limitations of feulgen microdensitometry. Chromosomes today. (In press.) NIRULA, 5., BHASKARAN, S., AND SWAMINATISAN, M. s. 1961. Effect of linear differentiation of chromosomes on the proportionality between chromosome length and DNA content. Exp. Cell Res., 24, 160-162. RRES, H., CAMERON, M. F., HAZARIKA, M. H., AND JONES, G. H. 1966. Nuclear variation between diploid angiosperms. Xature, 211, 828-830. ROTI-IFELS, K., SEXSMITH, E., HEIMBURGER, H., AND KRAUSE, M. o. 1966. Chromosome size and DNA content of species of Anemone L. and related genera (Ranum culaceae). Chromosoma, 20, 54-74. SUNDERLAND, N., AND MCLEISH, j. 1961. Nucleic acid content and concentration in root cells of higher plants. Exp. Cell Res., 24, 541-554.
TL;DR: There is evidence that current-induced volume flows which are normally considered to be electro-OSmosis have two components: a true electro-osmotic component due to frictional interaction and local osmosis due to a transport number effect.
TL;DR: In this paper, male and 81 female undergraduates wrote arguments favouring American intervention in South Vietnam and arguments opposing American intervention, and they had 5 minutes to write each set, and half the Ss wrote pro arguments fust, then con arguments.
Abstract: 77 male and 81 female undergraduates wrote arguments favouring American intervention in South Vietnam and arguments opposing American intervention. They had 5 minutes to write each set. Half the Ss wrote pro arguments fust, then con arguments. The other half did the reverse. Ss then completed an attitude scale concerned with American intervention, Rokeach's Dogmatism Scale, and Budner's test of intolerance of ambiguity. As predicted, Ss reported a greater number of arguments consistent with their attitude than arguments not consistent with their attitude. Contrary to prediction, Ss who were high in dogmatism or high in intolerance of ambiguity did not report relatively more consistent than inconsistent arguments when compared to Ss low in dogmatism or low in intolerance of ambiguity. Nor did they report a relatively low number of arguments inconsistent with attitude. Results were related to those of a previous study and discussed in terms of information reception and processing.
TL;DR: In this article, an attempt is made to answer the question whether distorting potentials that give really good fits to elastic scattering cross sections and total reaction cross sections for experiments in the relevant two-body subsystems can correctly give positions of maxima and minima in the (p, 2p) angular correlation.
01 Jan 1969
TL;DR: In this article, the steady rotation of an insulating body of revolution in an unbounded electrically conducting fluid permeated by a uniform axial applied magnetic field was investigated and an exact solution of the reduced equations in terms of an infinite series of Legendre polynomials of order 1 with coefficients which are the ratios of modified spherical Bessel functions was obtained.
Abstract: We investigate the steady rotation of an insulating body of revolution in an unbounded electrically conducting fluid permeated by a uniform axial applied magnetic field. The assumptions of a small magnetic Reynolds number (Rm ≪ 1, i.e. the weakly conducting situation) and negligible inertia forces compared with the magnetic forces (R/M2 ≪ 1) permit us to suppress the inflow at the poles and outflow at the equator, which normally occurs for a non-conducting viscous fluid ((12), pp. 436–439). Thus in the case of the sphere, we find an exact solution of the reduced equations in terms of an infinite series of Legendre polynomials of order 1 with coefficients which are the ratios of modified spherical Bessel functions. This is the canonical problem by which results for arbitrary bodies of revolution are obtained.
TL;DR: Ribose 5-phosphorothioate was synthesized by allowing ribose to react with thiophosphoryl chloride in triethyl phosphate and the analogue was a substrate for 5- phosphoribosyl pyrophosphate synthetase from Ehrlich ascites-tumour cells.
Abstract: 1. 5-Phosphoribosyl 1-methylenediphosphonate was isolated after reaction of ribose 5-phosphate and O-adenylyl methylenediphosphonate with 5-phosphoribosyl pyrophosphate synthetase from Ehrlich ascites-tumour cells. 2. The analogue reacted with adenine phosphoribosyltransferase, hypoxanthine phosphoribosyltransferase and nicotinamide phosphoribosyltransferase [Km (analogue)/Km (5-phosphoribosyl pyrophosphate) 0·17, 0·19 and 6·3 respectively; Vmax. (analogue)/Vmax. (5-phosphoribosyl pyrophosphate) 0·011, 0·26 and 1·1 respectively]. 3. The analogue was not a substrate for 5-phosphoribosyl pyrophosphate amidotransferase or orotate phosphoribosyltransferase. 4. Ribose 5-phosphorothioate was synthesized by allowing ribose to react with thiophosphoryl chloride in triethyl phosphate. The analogue was a substrate for 5-phosphoribosyl pyrophosphate synthetase from Ehrlich ascites-tumour cells. When this reaction was coupled to either adenine phosphoribosyltransferase or hypoxanthine phosphoribosyltransferase, adenosine 5′-phosphorothioate or inosine 5′-phosphorothioate was formed respectively.
TL;DR: In this article, the authors developed mathematical models which simulate the ion uptake pattern for a growing plant root and assumed simple shapes for the root and sought, when possible, solutions for the diffusion equation in the region bounded internally by this surface.
Abstract: In developing mathematical models which simulate the ion uptake pattern for a growing plant root, previous authors have either
(a)
Assumed simple shapes for the root and sought, when possible, solutions for the diffusion equation in the region bounded internally by this surface; or
(b)
Assumed a source-sink model for the action of the root without regard to the shape that this model might imply.
TL;DR: In this paper, a model was proposed which invokes canyon growth in vertical dimensions by a combination of rim up-growth and axial downcutting, and it was suggested that these canyons may have been initiated immediately following the formation of New Guinea's present morphological outline, and that they have been maintained as conduits during surrounding continental shelf upgrowth and progradation.
TL;DR: The Crossroads of Liberalism as discussed by the authors investigates the relationship between the editors of The New Republic and the centres of political power, focusing on the relationship of the editors to the sources of power.
Abstract: Shortly before America's involvement in the First World War there appeared a series of works of social and cultural criticism remarkable for their range and sophistication. The familiar list includes Herbert Croly's The Promise of American Life (1909) and Progressive Democracy (1914), Walter Weyl's The New Democracy (1912), Walter Lippmann's Preface to Politics (1913) and Drift and Mastery (1914), Van Wyck Brooks's The Wine of the Puritans (1909), America's Coming of Age (1915), and H. G. Wells (1915), and Randolph Bourne's Youth and Life (1913), The Gary Schools (1916) and Education and Living (1917). The authors of these books were involved as well in the development of vehicles for social criticism such as The New Republic and The Seven Arts which continued and institutiona lized the preoccupations of their books. These facts of creativity and speculative ferment are obvious; what has proved more difficult is an assessment of the character and purpose of this body of social theory. While we are justified in thinking of these men as constituting a fairly coherent group?they knew and were influenced by each other and they joined in the creation of journals devoted to agreed ends?we have yet to clarify precisely what they were about. Most com monly these men are viewed as the theoretical wing of Progressivism, so that they are explained by whatever broad hypothesis is offered about the movement as a whole. Among the questions raised about them, accordingly, are those that one always asks of theoreticians in a political movement: were their formulations influential, did they shape policy, were they close to the sources of power? We have had, for example, a minor controversy about the precise influence of Croly's The Promise of American Life on Theodore Roosevelt's New Nationalism. Charles Forcey has settled this point in the course of writing a book largely concerned with the relationship of the editors of The New Republic to the centres of political power.1 1 Charles Forcey, The Crossroads of Liberalism (New York, 1961). Forcey cites and discusses other works which have raised the question of Croly's influence on Roosevelt. For other treatments of the social critics as Progressives writing books rather than working in politics or reform institutions, see George Mowry, The Era of Theodore Roosevelt (New York, 1958); Arthur Link, Woodrow Wilson and the Progressive Era (New York, 1954) and Richard Hofstadter, The Age of Reform (New York, 1955).
TL;DR: In this article, it was shown that the number of open sets in a non-discrete topology on a finite set with n elements is not greater than 3 × 2n-2.
Abstract: It has been shown by D. Stephen that the number N of open sets in a non-discrete topology on a finite set with n elements is not greater than 3 × 2n-2.We show that for admissable topologies on a finite group N ≦ 2n/r, where r is the least order of its non-trivial normal subgroups. This is clearly a sharper bound.
TL;DR: It is discussed that the rapidly‐mobilized RNA functions as a storage product which is hydrolysed and incorporated into fresh RNA in the adjacent embryo during germination.
Abstract: SUMMARY
A new method of nucleic acid staining based on acridine orange is described and applied to sections of wheat seed. The coleorhiza of the ungerminated seed has an RNA content as high as the embryonic leaves and roots. During the first 24 h of germination the RNA levels of the roots, leaves and scutellum remain relatively unchanged while that of the coleorhiza falls to a low level. The possibility is discussed that the rapidly-mobilized RNA functions as a storage product which is hydrolysed and incorporated into fresh RNA in the adjacent embryo during germination.
TL;DR: In this paper, an Australian validation study of the taylor manifest anxiety scale is presented, and the authors present a method to measure the effect of taylor scales on the subjective experience of anxiety.
Abstract: (1969). An Australian validation study of the taylor manifest anxiety scale. Australian Psychologist: Vol. 4, No. 2-3, pp. 182-186.