Flinders University

About: Flinders University is a education organization based out in Adelaide, South Australia, Australia. It is known for research contribution in the topics: Population & Health care. The organization has 12033 authors who have published 32831 publications receiving 973172 citations. The organization is also known as: Flinders University of South Australia.

Sample storage conditions significantly influence faecal microbiome profiles.

Abstract: Sequencing-based studies of the human faecal microbiota are increasingly common. Appropriate storage of sample material is essential to avoid the introduction of post-collection bias in microbial community composition. Rapid freezing to -80 °C is commonly considered to be best-practice. However, this is not feasible in many studies, particularly those involving sample collection in participants' homes. We determined the extent to which a range of stabilisation and storage strategies maintained the composition of faecal microbial community structure relative to freezing to -80 °C. Refrigeration at 4 °C, storage at ambient temperature, and the use of several common preservative buffers (RNAlater, OMNIgene.GUT, Tris-EDTA) were assessed relative to freezing. Following 72 hours of storage, faecal microbial composition was assessed by 16 S rRNA amplicon sequencing. Refrigeration was associated with no significant alteration in faecal microbiota diversity or composition. However, samples stored using other conditions showed substantial divergence compared to -80 °C control samples. Aside from refrigeration, the use of OMNIgene.GUT resulted in the least alteration, while the greatest change was seen in samples stored in Tris-EDTA buffer. The commercially available OMNIgene.GUT kit may provide an important alternative where refrigeration and cold chain transportation is not available.

Diatomaceous Lessons in Nanotechnology and Advanced Materials

Abstract: Diatoms occupy sunlit aquatic environments. From a materialsscientist’s point of view, these unicellular algae are microscopicsilica nanofabrication factories that use two overlapping valvessealed together by girdle bands to encase themselves in a silicafrustule. The frustule is created with 3D precision of tens ofnanometers, in a hierarchical manner, and with multifunctionalproperties (Fig. 1).

Police reform and the problem of trust

Abstract: Police reform is widely undertaken in developing and post-authoritarian countries. The starting point for analysis of this phenomenon, it is suggested, is the absence of public trust in police that characterizes police-community relations in these countries. Without public trust in police, ‘policing by consent’ is difficult or impossible and public safety suffers. The nature of trust is examined in general terms and related to the problem of trust in governance. Then, the problematic nature of trust of the police is considered; structural features as well as performance aspects are invoked to explain distrust of police. In the penultimate section, the question of how to build trustworthy police forces is examined in the light of what has been learnt about the difficulties of maintaining or establishing trust in police. Process as well as substantive improvements each play a role here. In addition to building trust, ways of institutionalizing distrust are needed. The article concludes by pointing to some i...