Francis Marion University

About: Francis Marion University is a education organization based out in Florence, South Carolina, United States. It is known for research contribution in the topics: Population & Blazar. The organization has 312 authors who have published 555 publications receiving 13041 citations. The organization is also known as: Francis Marion College.

Guidelines for the use and interpretation of assays for monitoring autophagy

Abstract: In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.

Multiepoch Very Long Baseline Array Observations of EGRET-detected Quasars and BL Lacertae Objects: Superluminal Motion of Gamma-Ray Bright Blazars

Abstract: We present the results of a program to monitor the structure of the radio emission in 42 ?-ray bright blazars (31 quasars and 11 BL Lac objects) with the Very Long Baseline Array (VLBA) at 43, 22, and occasionally 15 and 8.4 GHz, over the period from 1993 November to 1997 July. We determine proper motions in 33 sources and find that the apparent superluminal motions in ?-ray sources are much faster than for the general population of bright compact radio sources. This follows the strong dependence of the ?-ray flux on the level of relativistic beaming for both external radiation Compton and synchrotron self-Compton emission. There is a positive correlation (correlation coefficient r = 0.45) between the flux density of the VLBI core and the ?-ray flux and a moderate correlation (partial correlation coefficient r = 0.31) between ?-ray apparent luminosity and superluminal velocities of jet components, as expected if the ?-ray emission originates in a very compact region of the relativistic jet and is highly beamed. In 43% of the sources the jet bends by more than 20? on parsec scales, which is consistent with amplification by projection effects of modest actual changes in position angle. In 27 of the sources in the sample there is at least one non-core component that appears to be stationary during our observations. Different characteristics of stationary features close to and farther from the core lead us to suggest two different classes of stationary components: those within about 2 mas of the core, probably associated with standing hydrodynamical compressions, and those farther down the jet, which tend to be associated with bends in the jet.

Parsec-Scale Properties of Markarian 501

Abstract: We present the results of a high angular resolution study of the BL Lac object Markarian 501 in the radio band. We consider data taken at 14 different epochs, ranging between 1.6 and 22 GHz in frequency, and including new Space VLBI observations obtained on 2001 March 5 and 6 at 1.6 and 5 GHz. We study the kinematics of the parsec-scale jet and estimate its bulk velocity and orientation with respect to the line of sight. Limb-brightened structure in the jet is clearly visible in our data, and we discuss its possible origin in terms of velocity gradients in the jet. Quasi-simultaneous, multiwavelength observations allow us to map the spectral index distribution and to compare it to the jet morphology. Finally, we estimate the physical parameters of the parsec-scale jet.

Fragmentation of Andes-to-Amazon connectivity by hydropower dams

Abstract: Andes-to-Amazon river connectivity controls numerous natural and human systems in the greater Amazon. However, it is being rapidly altered by a wave of new hydropower development, the impacts of which have been previously underestimated. We document 142 dams existing or under construction and 160 proposed dams for rivers draining the Andean headwaters of the Amazon. Existing dams have fragmented the tributary networks of six of eight major Andean Amazon river basins. Proposed dams could result in significant losses in river connectivity in river mainstems of five of eight major systems—the Napo, Maranon, Ucayali, Beni, and Mamore. With a newly reported 671 freshwater fish species inhabiting the Andean headwaters of the Amazon (>500 m), dams threaten previously unrecognized biodiversity, particularly among endemic and migratory species. Because Andean rivers contribute most of the sediment in the mainstem Amazon, losses in river connectivity translate to drastic alteration of river channel and floodplain geomorphology and associated ecosystem services.