Showing papers by "French Institute of Health and Medical Research published in 1968"
TL;DR: Investigations have led to the isolation of dialysable glycopeptides, which produce the common antigenicity of structural glycoproteins extracted from human or bovine heart valves and parietal polysaccharides of haematolytic group A streptococci.
Abstract: THERE is evidence of immunological cross-reaction between structural glycoproteins extracted from human or bovine heart valves and parietal polysaccharides of haematolytic group A streptococci1,2. Further investigations have led to the isolation of dialysable glycopeptides, which produce the common antigenicity.
103 citations
TL;DR: The results indicate that the RNA polymerase is bound to the DNA in the aggregate enzyme as part of an initiation or transcription complex, and the small amount of A-U-rich RNA which is synthesized at 37° is immediately destroyed by degradative enzymes, whereas the G-C-richRNA is much more resistant.
95 citations
TL;DR: Although it was able to confirm that rat liver chromatin was capable of binding as many RNA polymerase molecules as deproteinized rat liver DNA, a relative increase in the template efficiency of the chromatin as its concentration was decreased in the incubation mixture brings into question the exact physiological meaning of the template properties of isolated rat liver Chromatin.
51 citations
TL;DR: In these species, where the isoenzyme distribution in muscle differs widely between the foetus and the adult, a “foetal-like pattern” of muscle isoenzymes is found in acquired as well as in hereditary muscular diseases.
49 citations
TL;DR: The phosphoribosyltransferase activity (with hypoxanthine, guanine and adenine) of human red blood cells has been reported and the values observed showed no significant variation with age (within two days and 60 years) and sex.
43 citations
TL;DR: La possibilite d'autres remaniements est egalement discutee pour expliquer la perte du 17 et the formation du marqueur, which pourrait representer la moitie des evolutions clonales au cours des LMC.
38 citations
TL;DR: No differences appeared in the molecular structure of pyruvate kinase in normal and deficient cells, and the transition factors are discussed.
38 citations
TL;DR: The influence of the quantity of 32P given is very significant for equivalent clinical situations, the influence of white cell count and spleen size is significant for equal doses of32P, and these two types of factors favor the early occurrence of hematologic complications.
37 citations
TL;DR: The thyroid of an 8-yr-old girl with a congenital goiter without hypothyroidism has been studied: this defect explained the presence of free iodotyrosines in blood and urine.
Abstract: The thyroid of an 8-yr-old girl with a congenital goiter without hypothyroidism has been studied. She presented the same biochemical symptoms as her brother, whose goiter was studied previously (J Clin Endocr 27: 185, 1967). Iodinated serum proteins, of which an iodinated iodothyronine-containing serum albumin was the major component, were the predominant soluble iodinated proteins of the goiter. Poorly iodinated thyroglobulin accounted for only around 10% of total goiter iodoproteins. Qualitative and quantitative immunoprecipitation tests performed on the total soluble 14C-proteins synthesized during in vitro incubation of goiter slices in the presence of 14C-aminoacids failed to show the synthesis of 14C-serum albumin. A lack of DIT-deiodinating activity of the thyroid tissue in vitro has also been demonstrated: this defect explained the presence of free iodotyrosines in blood and urine. In this type of familial goiter, which combines 2 abnormalities (decrease or absence of thyroglobulin synthe...
34 citations
TL;DR: Values for the concentrations of oxidized and reduced pyridine nucleotides in rat liver and in rat and human blood are presented and are in fairly good agreement with those of Lowry.
Abstract: A fluorometric method is described for the estimation of di- and triphosphopyridine nucleotides (oxidized and reduced) in animal tissues.
The continuous cycling reduction of DPN+ (or TPN+) in the presence of excess of ethanol and alcohol dehydrogenase (or glucose-6-phosphate and glucose-6-phosphate dehydrogenase) was coupled with non enzymatic reoxidation of pyridine nucleotides with phenazine methosulfate.
Phenazine methosulfate reduced the non fluorescent compound, resazurine to the highly fluorescent compound, resorufine. The rate of production of resorufine is proportionnal to the concentration of pyridine nucleotides.
The procedure is relatively simple and requires only two commercial enzyme preparations for complete determination. This method can be used for estimation of pyridine nucleotides in concentrations as low as 0.1 μM and quantities as small as 10 pmoles.
The acid and alkaline extraction procedure was carried out essentially according to the method of Lowry. Conditions affecting the specificity, accuracy and reliability have been studied. During extraction, interfering material is formed, inhibiting the rate of reduction. A calibration procedure is described which overcomes this difficulty. Values for the concentrations of oxidized and reduced pyridine nucleotides in rat liver and in rat and human blood are presented and are in fairly good agreement with those of Lowry.
28 citations
TL;DR: It is concluded that the post-Rolandic lesion in this patient led to partial deafferentation, which was enhanced during the slow stage of sleep, thereby leading to clinical and subclinical seizures.
Journal Article•
TL;DR: In vivo and in vitro experiments give evidence for the existence in the endometrium of tight binding site(s) having a capacity equivalent to tissue concentration and suggest the presence of other types of binding that are looser and of much larger capacity and operative at estradiol concentrations greater than the physiological level.
Abstract: After the injection of a physiological dose of radioactive estradiol, the endometrium of the castrated rat displays a greater uptake (per unit of weight or per cell) and a longer retention of the hormone than does the myometrium. In vivo and in vitro experiments give evidence for the existence in the endometrium of tight binding site(s) having a capacity equivalent to tissue concentration of approximately 7 x 10-9 M (in vitro) or 1 x 10-8 M (in vivo, after injection of 0.1 µg, a physiological dose). A tentative "tissue affinity constant" of approximately 7 x 1011 M-1 has been calculated from the results of in vitro experiments at very low estradiol concentrations. The results also suggest, in both endometrium and myometrium, the presence of other types of binding that are looser and of much larger capacity and operative at estradiol concentrations greater than the physiological level.
TL;DR: It is concluded that maturation of human thyroglobulin may be independent of changes in its iodine content, as well as in accessibility of tyrosine residues to in vitro iodination or nitration.
TL;DR: Results indicate that 17β-hydroxy-5α and β-androstan-3-one are good precursors of urinary 5α andβ-androstane-3α, 17 β-diol respectively, and it seems probable that they are intermediates in the formation of the androstanediols from testosterone whereas most urinary 17-oxosteroids deriving from testosterone are formed after dehydrogenation of testosterone to Δ4- androstene-3,17
TL;DR: In this paper, a quantification of decharges unitaires is performed to determine the degree of veille active, veille relaxee, and sommeil paradoxal in the cortex.
TL;DR: The authors report on the results obtained by use of a microradiographic technique in the examination of brain arteries, compared with other methods of morphological study of the white matter in the frontal lobe, optic radiations and the corpus callosum cerebral circulation.
Abstract: Publisher Summary The recent development of carotid and vertebral arteriography, the use of photographic techniques such as subtraction or logetron now enable us to study blood vessels of under 0.5 mm in diameter. By these means there is now an opportunity of examining on the angiogram the area of the lenticulo-striate or thalamic arteries, the meningeal blood vessels and the branches of the ophthalmic artery. These concepts of vascular anatomy can only be developed if our arteriographic data are compared with opaque injections or any other method of studying cerebral arteries where the results can be used for normal reference purposes. Among these methods, radiopaque injections with the possibility of microradiography hold an important place. The authors report on the results obtained by use of a microradiographic technique in the examination of brain arteries. They illustrate their report with some examples relating to vascularisation. This technique is compared with other methods of morphological study of the white matter in the frontal lobe, optic radiations and the corpus callosum cerebral circulation.
TL;DR: The conformational change of β-lactoglobulin A induced by sucrose is made apparent, in the absence of any enzymatic reaction, by titration and difference spectroscopy curves, which are all the more plausible since interactions in a macromolecule often go with changes in the tertiary and quaternary structure.
TL;DR: Le systeme oxaloacetate ⇌ malate joue un role important dans the reoxydation du NADH produit au cours du metabolisme de l'ethanol.
TL;DR: In this article, auteurs onte´tudie´les re´ponses visuelles and somesthe´siques of 20 sujets adultes sains pendant les diffe´rentes phases du sommeil and pendant l'obnubilation qui suit le re´veil.
TL;DR: Histones inhibit the cell-free synthesis of hemoglobin and the possible role of histones in the selection of messenger RNA's in differentiated cells is discussed.
TL;DR: A membrane homogenate from Reticulocytes incubated in the presence of reticulocyte cell sap and 4 mM Mg 2+ is able to perform an energy-dependent synthesis of hemoglobin.
TL;DR: Les elements de diagnostic differentiel au microscope electronique entre particules elementaires, microvillosites, et particules virales, sont discutes.
TL;DR: La viabilite cellulaire in vivo est satisfaisante : plus de 85 % des globules rouges marques sont encore en circulation 24 heures apres leur injection a un receveur compatible.
Abstract: Resume Un procede de conservation par congelation des hematies est decrit. Le glycerol a la concentration intracellulaire de 35 % est l'agent protecteur. La congelation se fait sans controle de vitesse de refroidissement. La temperature de stockage est de −85°C. La decongelation s'effectue lentement en 75 minutes. L'elimination du glycerol apres decongelation est falicitee par l'emploi de solutions hypertoniques de sorbitol. Les hematies lavees sont remises en suspension dans le plasma d'origine. A la fin des manipulations, on recupere plus de 95 % des hematies initiales. La viabilite cellulaire in vivo est satisfaisante : plus de 85 % des globules rouges marques sont encore en circulation 24 heures apres leur injection a un receveur compatible. La methode est realisable dans tous les Centres de Transfusion.
TL;DR: In group 2, the hepatic synthesis of bile salts was increased, and the age, sex distribution, duration of drainage, and daily alimentary intake were not statistically different between the two groups.
TL;DR: The densite epineuse as mentioned in this paper is defined as the number of epines presentes par 10 μ of longueur dendritique, a distribution comparable to that of the dendrites apicales and the basilaires.
TL;DR: The technique of Few is sensitivite, rapid and reproducible, and seems convenient for the measurement of cortisol secretion rate in clinical routine and appears to give similar results in reproducibility.
TL;DR: In this article, a methode de dosage du glycerol and du sorbitol dans le sang est presentee, a modification de la technique de J. M. Bailey fondee sur la mesure colorimetrique du formaldehyde libere par oxydation des fonctions alcools primaires.
Abstract: Resume Une methode de dosage du glycerol et du sorbitol dans le sang est presentee. C'est une modification de la technique de J. M. Bailey fondee sur la mesure colorimetrique du formaldehyde libere par oxydation des fonctions alcools primaires. L'erreur systematique est inferieure a 5 %. La methode a permis d'aborder l'etude du comportement du glycerol et du sorbitol en presence des hematies. L'absence de penetration du sorbitol dans le milieu intracellulaire s'oppose a la grande rapidite du transfert du glycerol a travers la membrane erythrocytaire.
TL;DR: In this paper, le metabolisme des colorants azoiques, and particulierement du methylorange, a eteetudie du point de vue de ses repercussions sur le metabolism of hepatique du rat.
TL;DR: A study of its significance has been undertaken with the use of special perforated cards: main orientation of the QRSsĚ loop, clinical diagnosis of the heart disease, and electrical diagnosis were correlated.
TL;DR: In this article, le delai d'acquisition d'un phenotype nouveau est de deux semaines en moyenne, and l'on peut demontrer qu'il ne s'agit pas de l'apport de gamma-globulines injectees au cours d'exsanguino-transfusion, de transfusion de sang, de leucocytes ou de plaquettes.
Abstract: Resume Les phenotypes Gm et Inv ont ete suivis chez dix leucemiques avant et apres greffe de moelle allogenique. Un malade a un phenotype inchange, huit acquierent un ou plusieurs facteurs, un presente un nouveau phenotype caracterise par la perte de deux facteurs. Le delai d'acquisition d'un phenotype nouveau est de deux semaines en moyenne. Dans certains cas, l'on peut demontrer qu'il ne s'agit pas de l'apport de gamma-globulines injectees au cours d'exsanguino-transfusion, de transfusion de sang, de leucocytes ou de plaquettes. En effet, d'une part, un malade a presente, durant dix-huit mois un phenotype ou les facteurs Inv(1 et 2) etaient absents alors que les genes correspondants faisaient partie de son genome primitif ; d'autre part, un deuxieme malade a acquis durant dix mois le phenotype Inv(1,–2), rarissime chez les caucasiens. Aucun des donneurs de moelle de ce patient ne possedait un phenotype prouvant la greffe d'une cellule immunologiquement competente provenant d'une transfusion. Chez ce malade, une greffe de peau et l'etude des groupes sanguins ont demontre la tolerance d'un greffon provenant d'un donneur de moelle. Deux hypotheses sont avancees : soit une mutation somatique, soit la greffe d'immunocytes apportes par les transfusions et beneficiant de la tolerance acquise. L'etude quantitative des molecules d'immunoglobulines marquees par ces facteurs genetiques met en relief l'augmentation progressive de la synthese de ces molecules. L'acquisition frequente du phenotype Inv(1,–2), difficilement expliquable par la genetique formelle, est discutee.