Showing papers by "French Institute of Health and Medical Research published in 1969"
71 citations
TL;DR: Different estrogen derivatives have been studied and there is some correlation between their affinity for limited capacity receptors and their uterotrophic activity, but with acoefficient smaller than that of the uterus.
Abstract: Interaction of estradiol with rat uterus “receptors” has been studied using tissue incubation. The reversibility of estradiol binding has been confirmed and 2 types of binders have been observed. The first have great affinity, limited capacity and high specificity. The other, having smaller affinity and nonlimited capacity in the range of concentration used (up to 1 × 10-5 M), can be treated as a “partition” system. Nontarget organs, such as muscle or fat, also incorporate estradiol as a partition system, but with acoefficient smaller than that of the uterus. Different estrogen derivatives have been studied and there is some correlation between their affinity for limited capacity receptors and their uterotrophic activity. After incubation at 37 C, there is no “8 S” cytoplasmic estrogen binder, whereas there is more estradiol incorporated into nuclei than after 2 C incubation when the “8 S receptor” is preserved. (Endocrinology 84: 108, 1969)
48 citations
TL;DR: It is concluded that the integrity of hydrophobic interactions within the enzyme molecule is not equally critical with respect to the two activities and that it is possible to operate a change in enzyme specificity by a controlled change in hydrophilic interactions.
47 citations
TL;DR: It is shown that the transferase molecule is modified in its structure, a heretofore undescribed finding in galactosemia, and the name of “Rennes” is tentatively given to this type.
43 citations
TL;DR: The hypothesis that the double-stranded configuration of the RNA molecule is essential for the induction of interferon is agreed with the hypothesis that it is necessary for viral-induced interferons to be induced in vitro.
28 citations
28 citations
TL;DR: It was found that smokers, especially those who inhale, have a lower blood urea than non-smokers, and the more they smoke the lower the ureal level, and no relationship was found between this uric acid level and the amount of tobacco smoked.
24 citations
TL;DR: Two cases of gonadoblastoma with 46,XY/45,X mosaicism are reported, one of which had a female phenotype and presented at 13 years with stunting of growth and lack of pubertal manifestations.
22 citations
TL;DR: The data here presented support the hypothesis that this stimulating activity was related to a blocking effect of this protein on the biological action of interferon, comparable to the stimulons previously described for adenoviruses.
Abstract: SummaryThe Moloney strain of mouse sarcoma virus (MSV) enhances the replication of mouse hepatitis virus (MHV) in Balb/c mouse embryonic fibroblasts (MEF Balb/c). This enhancing effect may explain ...
20 citations
TL;DR: The electrophoretic mobility of these enzyme bands support the hypothesis of aldolase “C” (brain type) being the third a Aldolase present in human liver.
19 citations
TL;DR: A concentrated KCl-supernatant is able to stimulate hemoglobin synthesis strongly in a reticulocyte cell-free system with intact ribosome, especially if the ribosomes are preincubated.
TL;DR: The tissue antagonist of interferon (TAI) as discussed by the authors is present in the acidosoluble fraction after precipitation with 5% trichloroacetic acid and is resistant to proteases, to heat, and to low pH.
Abstract: SummaryExtracts of the amniotic and chorionic membranes contain a factor which blocks the action of interferon. The anti-interferon effect can be induced when the antiviral state mediated by interferon is already established. The tissue antagonist of interferon (TAI) is present in the acidosoluble fraction after precipitation with 5% trichloroacetic acid. It does not dialyze or sediment after ultracentrifugation. It is resistant to proteases, to heat, and to low pH. Although the biological role of this substance is unknown, it is postulated that it may contribute to terminate an antiviral state in the tissue and restore cellular receptivity to viruses.
TL;DR: A preincubation of histones with acidic proteins strongly diminished the inhibition of hemoglobin biosynthesis by either protein, which confirms the hypothesis of complex formation between histones and acidic proteins.
TL;DR: It is concluded that there are two allelomorphs rabbit hemoglobins: Hb α 29 Val and Hbα 29 Leu, both of which are related to α-TIV hemoglobin.
TL;DR: The results clearly demonstrate that posthepatectomy steatosis is not produced by a deficiency in lipoproteins synthesis or liberation, and it is suggested that the rate of triglycerides synthesis temporarily exceeds the rates of their liberation.
TL;DR: The 3—8-S fraction of the total soluble thyroid proteins labelled by incubation in vitro of rat thyroid glands in the presence of [14C]amino acids has been purified by repeated (NH4)2SO4 precipitations and sucrose gradient centrifugations.
TL;DR: Thiamine interacts in vitro with acetylcholine, norepinephrine and serotonin yielding molecular complexes and can be related to the physiological effect of thiamine which, in large doses, induces a general synaptolysis.
TL;DR: It is shown that the diminished tolerance to glucose observed in azotaemic patients in presence of a large and prolonged insulin secretion cannot be related to abnormalities in growth hormone or glucagon secretion.
Abstract: This study confirms our first results showing large and prolonged insulin secretion in patients with azotaemia and glucose intolerance. It shows that the pattern of growth hormone and glucagon secretions was not modified in these well nourished patients with chronic renal diseases and hyperazotaemia : the fasting values were in the normal range and the response during a 5 h glucose tolerance test was normal, except in one man with Fabry's disease, in whom a very large increase in plasma growth hormone was observed at the beginning of the GTT when the blood glucose increased. There was no increase in plasma H.G. H. during the intravenous glucose tolerance test of 60 min. Plasma glucagon values were normal.-This study done on 11 subjects shows that the diminished tolerance to glucose observed in azotaemic patients in presence of a large and prolonged insulin secretion cannot be related to abnormalities in growth hormone or glucagon secretion.
TL;DR: This spot-test procedure is recommended for mass screening and diagnosis of red cell glutathione peroxidase deficiency and good agreement with quantitative determination of the enzyme activity by a spectro-photometric method was found.
TL;DR: The treatment of N-acylated peptides with anhydrous trifluoroacetic acid resulted in a cleavage of the first peptide bond with a rate which was dependent on the nature of the N- Tracy group, and the importance of amide participation in the reactions of polypeptides is discussed.
Patent•
27 Oct 1969TL;DR: In this paper, the authors propose a process for the MANUFACTURE of INTERFERON-RICH PREPARATIONS by INCUBATING HUMAN CELLS in the PRESENCE of an INDUCTION AGENT SUCH AS A VIRUS.
Abstract: THE INVENTION PROVIDES A PROCESS FOR THE MANUFACTURE OF INTERFERON-RICH PREPARATIONS BY INCUBATING HUMAN CELLS IN THE PRESENCE OF AN INDUCTION AGENT SUCH AS A VIRUS, AND SEPARATING THE INTERFERON FORMED.
TL;DR: In this paper, a rat liver nuclear aggregate RNA polymerase (NIRP) was used to synthesize RNA in vitro at high ionic strength and DNA-RNA hybridization was performed in vivo.
TL;DR: Electron microscopic examination of transverse and sagittal serial sections of a locally irradiated cell by a beam of ultraviolet light permitted the study of structure of the lesions and their correlation with morphologic alterations observed by light microscopy previously reported in the literature.
TL;DR: In this article, Wistar et al. showed that l'ethanol inhibe aussi la glycolyse and provient du fonctionnement du cycle des pentoses, that le pyruvate and l'oxaloacetate proviennent de l'aspartate.
01 Jan 1969
TL;DR: In order to try to elucidate the reasons for the prolongation of the cell cycle during the growth of an experimental ascites tumour of the C3H mouse, the present work reports certain modifications of the metabolism in 5 and 12-day ascite tumours.
TL;DR: Dans le foie foetal, l'aldolase A parait proche du taux du foie adulte, mais l'ALDolase B est egalement abaissee.