Showing papers by "Fu Jen Catholic University published in 2008"

Positive correlations of Oct-4 and Nanog in oral cancer stem-like cells and high-grade oral squamous cell carcinoma.

Abstract: Purpose: Oral squamous cell carcinoma (OSCC), like many solid tumors, contains a heterogeneous population of cancer cells. Recent data suggest that a rare subpopulation of cancer cells, termed cancer stem cells (CSC), is capable of initiating, maintaining, and expanding the growth of tumor. Identification and characterization of CSC from OSCC facilitates the monitoring, therapy, or prevention of OSCC. Experimental Design: We enriched oral cancer stem-like cells (OC-SLC) through sphere formation by cultivating OSCC cells from established OSCC cell lines or primary cultures of OSCC patients within defined serum-free medium. Differential expression profile of stemness genes between enriched OC-SLC and parental OSCC was elucidated. Furthermore, immunohistochemical staining of stemness markers on OSCC patient tissues was examined to evaluate the association between stemness genes and prognosis of OSCC. Results: Enriched OC-SLC highly expressed the stem/progenitor cell markers and ABC transporter gene (Oct-4, Nanog, CD117, Nestin, CD133, and ABCG2) and also displayed induced differentiation abilities and enhanced migration/invasion/malignancy capabilities in vitro and in vivo . Elevated expression of CD133 was shown in the enriched OC-SLC from OSCC patients9 tumors. Positive correlations of Oct-4, Nanog, or CD133 expression on tumor stage were shown on 52 OSCC patient tissues. Kaplan-Meier analyses exhibited that Nanog/Oct-4/CD133 triple-positive patients predicted the worst survival prognosis of OSCC patients. Conclusion: We enriched a subpopulation of cancer stem-like cell from OSCC by sphere formation. The enriched OC-SLC possesses the characteristics of both stem cells and malignant tumors. Additionally, expression of stemness markers (Nanog/Oct-4/CD133) contradicts the survival prognosis of OSCC patients.

Effects of theatrical elements on experiential quality and loyalty intentions for theme parks.

Abstract: Issues surrounding hedonic consumption, experience economy and experiential marketing have become highly important in recent years. This study applies consumption experience and theater theory and proposes a model to explore the effects of theatrical elements of theme parks (including attractiveness of scripts, charm of setting, planning of activities and consistency of theme) on consumers' experiential quality (including immersion, surprise, participation and fun), experiential satisfaction and loyalty intentions. A survey composed of 408 customers in Hualien Ocean Park was conducted in Taiwan. The results of structural equation modeling indicate that most theatrical elements relate positively to experiential quality and that experiential quality in turn relates positively to experiential satisfaction. Furthermore, experiential satisfaction is positively related to loyalty intentions. Managers of theme parks could focus on improving the theatrical elements to satisfy customers and gain loyalty.

Observation of two resonancelike structures: Observation of two resonancelike structures in the Ic1 mass distribution in exclusive BA0K-I+Ic1

Abstract: Reference EPFL-ARTICLE-154418doi:10.1103/PhysRevD.78.072004View record in Web of Science Record created on 2010-11-05, modified on 2017-12-10

Transcranial color doppler study for reversible cerebral vasoconstriction syndromes

Abstract: Objective Reversible cerebral vasoconstriction syndromes (RCVS) are characterized by thunderclap headaches and reversible cerebral vasoconstrictions. No systematic studies on cerebral hemodynamics have been published. Methods Patients with RCVS were consecutively recruited. Sequential transcranial color-coded sonography studies were performed on the middle cerebral artery (MCA) for 3 months. Mean flow velocities (VMCA) and Lindegaard Index (LI) were recorded and compared with those of controls. Results Thirty-two patients (all female; average age, 49.7 ± 6.8 years) were enrolled. Four developed of reversible posterior leukoencephalopathy syndrome, and two of them, ischemic strokes. One hundred and twenty-six sonography studies were performed on 57 eligible MCAs. The mean maximum VMCA (109.5 ± 30.8cm/sec) and LI (2.2 ± 0.7) of RCVS patients exceeded those of controls (VMCA: 66.3 ± 9.5cm/sec, p < 0.001; LI: 1.4 ± 0.3, p < 0.001). The VMCA and LI levels were still at their plateau at the mean time (day 22 after headache onset) of headache resolution. Fifteen (46.9%) patients had VMCA exceeding 120cm/sec, and 5 (16%) had LI exceeding 3. Patients fulfilling the criteria of subarachnoid hemorrhage mild vasospasm (n = 4; 13%), that is, both VMCA greater than 120cm/sec and LI greater than 3, had a greater risk of posterior leukoencephalopathy (75 vs 4%; p = 0.003) and ischemic strokes (50 vs 0%; p = 0.01) than those without. Interpretation Patients with RCVS experienced prolonged vasoconstriction, making the risk for posterior leukoencephalopathy and ischemic strokes outlast headache resolution. Patients fulfilling mild vasospasm criteria for subarachnoid hemorrhage carry a high risk. Ann Neurol 2008

Technology integration applied to project‐based learning in science

Abstract: This paper reports the findings of a study which observed students’ (aged 10–11) use of technology during project‐based learning activities in science. As part of the overall process of project‐based learning, students used computer technology as a tool for collecting information, organising it and presenting it to their peers. Students conducted research (through guided research processes), interacted with peers, teachers and the community (through personal interviews and visits), and displayed their understanding of knowledge through the presentation of web‐pages. The results of the study indicate that all of the students achieved their research goals. Students’ learning outcomes were observed based on their achievements in relation to developing skills and ability to synthesise and elaborate knowledge, to engage in scientific exploratory tasks, and to use the technology for supporting and reporting their research work. Teacher’s support in relation to providing coaching skills is crucial to students’ s...

Soft Computing for Battery State-of-Charge (BSOC) Estimation in Battery String Systems

Abstract: In this paper, a soft computing technique for estimating battery state-of-charge of individual batteries in a battery string is proposed. The soft computing approach uses a fusion of a fuzzy neural network (FNN) with B-spline membership functions (BMFs) and a reduced-form genetic algorithm (RGA). The algorithm is employed to tune both control points of the BMFs and the weights of the FNNs. The traditional multiple-input multiple-output FNN (MIMOFNN) cannot directly be used in this paper. The main reason is that there are too many free parameters in the MIMOFNN to be trained if many inputs are required. In this paper, a merged multiple-input single-output (MISO) FNN is proposed and can be trained by the RGA optimization approach. The merged MISO FNN with RGA (FNNRGA) can achieve faster convergence and lower estimation error than neural networks with the back propagation method. From experimental results, the proposed merged MISO FNNRGA is superior, more robust than the traditional method, and the overfitting suppression features are significantly improved.

Observation of a new DsJ meson in B+-->D0D0K+ decays.

Abstract: We report the observation of a new DsJ meson produced in B+-->D0DsJ-->D0D0K+. This state has a mass of M=2708+/-9(-10)(+11) MeV/c2, a width Gamma=108+/-23(-31)(+36) MeV/c2 and a 1- spin-parity. The statistical significance of this observation is 8.4 sigma. The results are based on an analysis of 449 x 10(6) BB events collected at the Upsilon(4S) resonance with the Belle detector at the KEKB asymmetric-energy e+e- collider.

Automatic room light intensity detection and control using a microprocessor and light sensors

Abstract: In this paper we propose a design using both a microprocessor and light sensors for automatic room light detection and control. Our design, the HLCM (home light control module) which will be installed in every light fixture of a family, is made up of four blocks: the pyroelectric infrared (PIR) sensor circuit, the light sensor circuit, the microprocessor and the RF module. By using the PIR sensor circuit, the HLCM detects if a human body enters the detection area or not. If there is no human body present, all controlled lights are turned off. If there is, the HLCM detects the light intensity under the environment and maintains sufficient light by controlling the number of lights. We have also integrated an RF module to transmit and receive the data from each HLCM so we can control different lights in different regions. The result of using the HLCM shows that the total power consumption can be reduced.

Delivery of resveratrol, a red wine polyphenol, from solutions and hydrogels via the skin.

Abstract: Resveratrol, the main active polyphenol in red wine, has been demonstrated to show benefits against skin disorders. The bioavailability of orally administered resveratrol is insufficient to permit high enough drug concentrations for systemic therapy. In this study, we examined the feasibility of the topical/transdermal delivery of resveratrol. The effects of vehicles on the in vitro permeation and skin deposition from saturated solutions such as aqueous buffers and soybean oil were investigated. The general trend for the delivery from solutions was: pH 6 buffer=pH 8 buffer>10% glycerol formal in pH 6 buffer>pH 9.9 buffer>pH 10.8 buffer>soybean oil. A linear relationship was established between the permeability coefficient (K(p)) and drug accumulation in the skin reservoir. Viable epidermis/dermis served as the predominant barrier for non-ionic resveratrol permeation. On the other hand, both the stratum corneum (SC) and viable skin acted as barriers to anionic resveratrol. Several prototype hydrogel systems were also studied as resveratrol vehicles. The viscosity but not the polarity of the hydrogels controlled resveratrol permeation/deposition. Piceatannol, a derivative of resveratrol with high pharmacological activity, showed 11.6-fold lower skin permeation compared to resveratrol. The safety profiles of resveratrol suggested that the hydrogel caused no SC disruption or skin erythema. It was concluded that delivery via a skin route may be a potent way to achieve the therapeutic effects of resveratrol. This is the first report to establish the permeation profiles for topically applied resveratrol.

Ortholog-based protein-protein interaction prediction and its application to inter-species interactions.

Abstract: The rapid growth of protein-protein interaction (PPI) data has led to the emergence of PPI network analysis. Despite advances in high-throughput techniques, the interactomes of several model organisms are still far from complete. Therefore, it is desirable to expand these interactomes with ortholog-based and other methods. Orthologous pairs of 18 eukaryotic species were expanded and merged with experimental PPI datasets. The contributions of interologs from each species were evaluated. The expanded orthologous pairs enable the inference of interologs for various species. For example, more than 32,000 human interactions can be predicted. The same dataset has also been applied to the prediction of host-pathogen interactions. PPIs between P. falciparum calmodulin and several H. sapiens proteins are predicted, and these interactions may contribute to the maintenance of host cell Ca2+ concentration. Using comparisons with Bayesian and structure-based approaches, interactions between putative HSP40 homologs of P. falciparum and the H. sapiens TNF receptor associated factor family are revealed, suggesting a role for these interactions in the interference of the human immune response to P. falciparum. The PPI datasets are available from POINT http://point.bioinformatics.tw/ and POINeT http://poinet.bioinformatics.tw/ . Further development of methods to predict host-pathogen interactions should incorporate multiple approaches in order to improve sensitivity, and should facilitate the identification of targets for drug discovery and design.

Personality and motivations for playing online games.

Abstract: Using the five-factor model of personality and a previously theorized motivation typology, the authors investigated the relationship between personality and motivation for playing online games. The results indicated that openness was positively related to discovery and role- playing motivation, and conscientiousness was positively related to escapism motivation. In addition, extraversion was positively related to teamwork motivation, and agreeableness was positively related to advancement motivation. However, neuroticism was negatively related to teamwork motivation. These findings have several implications and could provide further research directions.

Training, Satisfaction with Internship Programs, and Confidence about Future Careers among Hospitality Students: A Case Study of Universities in Taiwan

Abstract: The purpose of this research is to investigate the factors associated with hospitality students' satisfaction with internship programs and the relationships between training, job satisfaction, and confidence about future careers in order to provide schools and industries with suggestions regarding course development and training during internships. A principal component analysis was used to determine the degree of satisfaction with the supervisor, the educational program, the environment, the administration, and relationships. Training classes made a significant difference in satisfaction with the supervisor and educational program. The regression analysis showed that training satisfaction played a positive role as a predictor of participants' job satisfaction and confidence about future careers. The effect of satisfaction with training on participants' confidence about future careers was mediated by their satisfaction with the internship. The administration and learning factors in connection wit...

Involvement of human CD44 during Cryptococcus neoformans infection of brain microvascular endothelial cells

Abstract: Pathogenic yeast Cryptococcus neoformans causes devastating cryptococcal meningoencephalitis. Our previous studies demonstrated that C. neoformans hyaluronic acid was required for invasion into human brain microvascular endothelial cells (HBMEC), which constitute the blood-brain barrier. In this report, we demonstrate that C. neoformans hyaluronic acid interacts with CD44 on HBMEC. Our results suggest that HBMEC CD44 is a primary receptor during C. neoformans infection, based on the following observations. First, anti-CD44 neutralizing antibody treatment was able to significantly reduce C. neoformans association with HBMEC. Second, C. neoformans association was considerably impaired using either CD44-knock-down HBMEC or C. neoformans hyaluronic acid-deficient strains. Third, overexpression of CD44 in HBMEC increased their association activity towards C. neoformans. Fourth, confocal microscopic images showed that CD44 was enriched at and around the C. neoformans association sites. Fifth, upon C. neoformans and HBMEC engagement, a subpopulation of CD44 and actin translocated to the host membrane rafts. Our results highlight the interactions between C. neoformans hyaluronic acid and host CD44 and the dynamic results of these interactions, which may represent events during the adhesion and entry of C. neoformans at HBMEC membrane rafts.

Heart rate variability measures as predictors of in-hospital mortality in ED patients with sepsis.

Abstract: Objective To determine the predictive capability of heart rate variability (HRV) measures of patients with sepsis in the ED for in-hospital death. Methods This was a prospective, observational study. A consecutive cohort of patients visiting the ED of a university teaching hospital who met the criteria of sepsis over a 6-month period were enrolled in this study. General demographics, vital signs, laboratory data, and Mortality in Emergency Department Sepsis score were obtained in the ED; the in-patient medical record was reviewed; and a series of continuous 10-minute electrocardiographic signals were recorded for off-line HRV analysis to assess the in-hospital mortality of the patients. Results One hundred thirty-two patients aged 27 to 86 years who met the inclusion criteria were enrolled. According to the in-hospital outcome, the patients were categorized into 2 groups: nonsurvivors (n = 10) and survivors (n = 122). The baseline HRV measures, including SDNN, TP, VLFP, LFP, and LFP/HFP ratio, of nonsurvivors were significantly lower, whereas the nHFP was significantly higher, than those of survivors. Multiple logistic regression model identified SDNN and nHFP as the significant independent variables in the prediction of in-hospital mortality for ED patients with sepsis. The receiver operating characteristic area for SDNN and nHFP in predicting the risk of death was 0.700 and 0.739, respectively. Conclusions Heart rate variability measures, especially the SDNN and nHFP, may be used as valuable predictors of in-hospital mortality in patients with sepsis attending the ED.

PhosphoPOINT: a comprehensive human kinase interactome and phospho-protein database.

Abstract: Motivation: To fully understand how a protein kinase regulates biological processes, it is imperative to first identify its substrate(s) and interacting protein(s). However, of the 518 known human serine/threonine/tyrosine kinases, 35% of these have known substrates, while 14% of the kinases have identified substrate recognition motifs. In contrast, 85% of the kinases have protein–protein interaction (PPI) datasets, raising the possibility that we might reveal potential kinase–substrate pairs from these PPIs. Results: PhosphoPOINT, a comprehensive human kinase interactome and phospho-protein database, is a collection of 4195 phospho-proteins with a total of 15 738 phosphorylation sites. PhosphoPOINT annotates the interactions among kinases, with their down-stream substrates and with interacting (phospho)-proteins to modulate the kinase–substrate pairs. PhosphoPOINT implements various gene expression profiles and Gene Ontology cellular component information to evaluate each kinase and their interacting (phospho)-proteins/substrates. Integration of cSNPs that cause amino acids change with the proteins with the phosphoprotein dataset reveals that 64 phosphorylation sites result in a disease phenotypes when changed; the linked phenotypes include schizophrenia and hypertension. PhosphoPOINT also provides a search function for all phospho-peptides using about 300 known kinase/phosphatase substrate/binding motifs. Altogether, PhosphoPOINT provides robust annotation for kinases, their downstream substrates and their interaction (phospho)-proteins and this should accelerate the functional characterization of kinomemediated signaling. Availability: PhosphoPOINT can be freely accessed in http://kinase. bioinformatics.tw/

Difference in direct charge-parity violation between charged and neutral B meson decays.

Abstract: Equal amounts of matter and antimatter are predicted to have been produced in the Big Bang, but our observable Universe is clearly matter-dominated. One of the prerequisites for understanding this elimination of antimatter is the nonconservation of charge-parity (CP) symmetry. So far, two types of CP violation have been observed in the neutral K meson (K(0)) and B meson (B(0)) systems: CP violation involving the mixing between K(0) and its antiparticle (and likewise for B(0) and ), and direct CP violation in the decay of each meson. The observed effects for both types of CP violation are substantially larger for the B(0) meson system. However, they are still consistent with the standard model of particle physics, which has a unique source of CP violation that is known to be too small to account for the matter-dominated Universe. Here we report that the direct CP violation in charged B(+/-)-->K(+/-)pi(0) decay is different from that in the neutral B(0) counterpart. The direct CP-violating decay rate asymmetry, (that is, the difference between the number of observed B(-)-->K(-)pi(0) event versus B(+)-->K(+) pi(0) events, normalized to the sum of these events) is measured to be about +7%, with an uncertainty that is reduced by a factor of 1.7 from a previous measurement. However, the asymmetry for versus B(0)-->K(+)pi(-) is at the -10% level. Although it is susceptible to strong interaction effects that need further clarification, this large deviation in direct CP violation between charged and neutral B meson decays could be an indication of new sources of CP violation-which would help to explain the dominance of matter in the Universe.

Mechanisms of compartmentalized expression of Mrg class G-protein-coupled sensory receptors.

Abstract: Mrg class G-protein-coupled receptors (GPCRs) are expressed exclusively in sensory neurons in the trigeminal and dorsal root ganglia. Pharmacological activation of Mrg proteins is capable of modulating sensory neuron activities and elicits nociceptive effects. In this study, we illustrate a control mechanism that allows the Runx1 runt domain transcription factor to generate compartmentalized expression of these sensory GPCRs. Expression of MrgA, MrgB, and MrgC subclasses is confined to an “A/B/C” neuronal compartment that expresses Runx1 transiently (or does not express Runx1), whereas MrgD expression is restricted to a “D” compartment with persistent Runx1 expression. Runx1 is initially required for the expression of all Mrg genes. However, during late development Runx1 becomes a repressor for MrgA/B/C genes. As a result, MrgA/B/C expression persists only in the Runx1^− “A/B/C” compartment. In Δ446 mice, in which Runx1 lacks the C-terminal repression domain, expression of MrgA/B/C genes is dramatically expanded into the Runx1^+ “D” compartment. MrgD expression, however, is resistant to Runx1-mediated repression in the “D” compartment. Therefore, the creation of Runx1+ and Runx1^− compartments, in conjunction with different responses of Mrg genes to Runx1-mediated repression, results in the compartmentalized expression of MrgA/B/C versus MrgD genes. Within the MrgA/B/C compartment, MrgB4-expressing neurons innervate exclusively the hairy skin. Here we found that Smad4, a downstream component of bone morphological protein-mediated signaling, is required selectively for the expression of MrgB4. Our study suggests a new line of evidence that specification of sensory subtypes is established progressively during perinatal and postnatal development.

Arsenic trioxide impairs spermatogenesis via reducing gene expression levels in testosterone synthesis pathway.

Abstract: Arsenic trioxide (As2O3) has recently received a great deal of attention because of its capacity to cause complete remission of acute promyelocytic leukemia (APL). To evaluate possible toxicity on the male reproductive system during arsenic therapy, male mice were used as a model. Outbred mice (ICR/CD1 and S-W, 6 weeks old) were subcutaneously administered As2O3 continuously for 5 days, with a 2-day interval, for a period of 3 weeks. As2O3 doses were 0, 0.15, 0.3, 1.5, and 3.0 mg/kg of body weight, respectively. No mice died in any dosage group. Our data showed no significant changes in food consumption or in the weight of the body, liver, testis, or epididymis after As2O3 treatment. Using histological observation to identify the stages of seminiferous tubules, we showed that As2O3 treatment resulted in the inhibition of spermatogenesis. The frequency of mature seminiferous tubules (stages VII and VIII) was markedly decreased after As2O3 treatment. A significant decrease in sperm motility and viability also was found with computer-assisted sperm analysis (CASA) and a SYBR14/PI staining assay. Using an enzyme-linked immunosorbent assay (ELISA), we found a significant decrease in levels of plasma luteinizing hormone (LH) at a dose of 3.0 mg/kg body weight. No significant difference was found in plasma follicle-stimulating hormone (FSH) in all dosages. A significant decrease was found in plasma testosterone in all dosages, but no difference in intratesticular testosterone, with the exception of As2O3 at a dose of 3.0 mg/kg body weight. Moreover, there was a significant decrease in the levels of mRNA involved in testicular testosterone synthesis, cytochrome P450 side chain cleavage (P450scc), 3beta-hydroxysteroid dehydrogenase (3beta-HSD), and cytochrome P450 17-alpha hydroxylase/C17-20 lyase (Cyp17). The use of immunohistological observation showed no obvious difference in the testosterone level of Leydig cells of mice treated with As2O3 at doses of 0.3 and 1.5 mg/kg body weight. We concluded that As2O3 treatment caused damage to sperm mobility and viability. As2O3 treatment disturbed spermatogenesis via reducing gene expression of the key enzymes in testosterone synthesis.

Chinese Version of the Zarit Caregiver Burden Interview: A Validation Study

Abstract: Objective The prevalence of dementia is expected to increase in Chinese society in the next 40 years. Family members remain the primary caregivers and bear a heavy burden. The present study aims to examine the reliability and validity of the Chinese version of the Zarit Burden Interview (CZBI). Method One hundred eighty-one patient-caregiver dyads were recruited. Caregivers completed the measures of the CZBI, Caregiver Activity Survey (CAS) and 12-item Chinese Health Questionnaire (CHQ-12). Mini-Mental State Examination and Clinical Dementia Rating Scale were used to assess the patients' condition. Results Overall reliability of CZBI was good, with the internal consistency and test–retest reliability being high. The CZBI was highly correlated with the scores of the CAS and CHQ-12, which provides evidence of concurrent validity. Factor analysis found five factors, which accounted for 60% of variance. Conclusion The CZBI is feasible for measuring feelings of burden of caregivers for Taiwanese patients with dementia.

Power Monitoring and Control for Electric Home Appliances Based on Power Line Communication

Abstract: Home power consumption tends to grow in proportion to the increase in the number of large-sized electric home appliances. An embedded system without any new additional wiring has been developed for home power management. By using power line communication (PLC) technology, electric home appliances can be controlled and monitored through domestic power lines. We describe a PPCOM (PTC Power-Controlled Outlet Module) which integrates the multiple AC power sockets, the power measuring module, the PTC module and a microcontroller into a power outlet to switch the power of the sockets on/off and to measure the power consumption of plugged-in electric home appliances. We have also designed an embedded home server which supports the Web page user interface, thus allowing the user to easily control and monitor the electric home appliances by means of the Internet. In addition, the field experiments reported have demonstrated that our design can be practically implemented and provides adequate results.