Showing papers by "Fundación Instituto Leloir published in 2004"
TL;DR: CD9, CD63, CD81, and CD82 could play a role in modulating the interactions between immature DCs and their environment, slowing their migratory ability, but only CD63 would intervene in the internalization of complex antigens.
133 citations
TL;DR: A hypothesis in which specific features of newly generated neurons contribute to hippocampal plasticity and function is proposed and the most recent and relevant findings are discussed in the context of the proposed hypothesis.
Abstract: The functional relevance of adult hippocampal neurogenesis has long been a matter of intense experimentation and debate, but the precise role of new neurons has not been sufficiently elaborated. Here we propose a hypothesis in which specific features of newly generated neurons contribute to hippocampal plasticity and function and discuss the most recent and relevant findings in the context of the proposed hypothesis.
116 citations
TL;DR: It was concluded that the GT-derived quality control may be operative with nearly native conformers and that no alternative ER-retaining mechanisms are required when glycoproteins approach their proper folding.
116 citations
TL;DR: It is demonstrated that endogenous hippocampal IL‐1 specifically modulates a fear‐motivated learning task, and suggested that IL‐ 1α activity in the CNS is part of the hippocampal memory processing.
Abstract: The interleukin-1 (IL-1) cytokine family (IL-1, IL-, and the IL-1 receptor antagonist) is involved in immune and inflammatory responses both in the brain and in the periphery. Recently, it has also been shown to influence behavior and memory consolidation. However, within the experimental systems studied, it has remained unclear whether the role of IL-1 is associated solely with a pathophysiological process or whether it is a neuromodulator in normal adult brain. To evaluate the involvement of the nonpathological endogenous IL-1 system in learning, we studied the expression of IL-1, IL-1, and IL-1ra during memory consolidation. We observed a learning-specific hippocampal IL-1 mRNA induction, but not that of IL-1 or IL-1ra mRNAs, after inhibitory avoidance training. Moreover, when IL-1 receptor activity was inhibited using an adenoviral vector that expresses the IL-1 receptor antagonist (IL-1ra) in the hippocampus, both short-term and long-term memory retention scores were facilitated. In contrast, endogenous hippocampal IL-1 played no role in the habituation to a novel environment. These results demonstrate that endogenous hippocampal IL-1 specifically modulates a fear-motivated learning task, and suggest that IL-1 activity in the CNS is part of the hippocampal memory processing.
99 citations
TL;DR: This is the first dissected example of a transcription factor functioning as a phosphorylation-activated switch for a cold-shock gene, allowing the cell to optimize the fluidity of membrane phospholipids.
77 citations
TL;DR: A modular system is established to produce chimeric proteins decorated with ten copies of a desired peptide as long as 27 residues and it is shown that their folding and stability is similar to that of the wild‐type protein.
Abstract: Protein assemblies with a high degree of repetitiveness and organization are known to induce strong immune responses. For that reason they have been postulated for the design of subunit vaccines by means of protein engineering. The enzyme lumazine synthase from Brucella spp. (BLS) is highly immunogenic, presumably owing to its homodecameric arrangement and remarkable thermodynamic stability. Structural analysis has shown that it is possible to insert foreign peptides at the ten amino terminus of BLS without disrupting its general folding. These peptides would be displayed to the immune system in a highly symmetric three-dimensional array. In the present work, BLS has been used as a protein carrier of foreign peptides. We have established a modular system to produce chimeric proteins decorated with ten copies of a desired peptide as long as 27 residues and have shown that their folding and stability is similar to that of the wild-type protein. The knowledge about the mechanisms of dissociation and unfolding of BLS allowed the engineering of polyvalent chimeras displaying different predefined peptides on the same molecular scaffold. Moreover, the reassembly of mixtures of chimeras at different steps of the unfolding process was used to control the stoichiometry and spatial arrangement for the simultaneous display of different peptides on BLS. This strategy would be useful for vaccine development and other biomedical applications.
73 citations
TL;DR: It is suggested that synaptogenesis is a necessary process for polarization in PIP3 pathway mediated by the PTEN catalytic-fragment into dendrites of CNS neurons.
39 citations
TL;DR: In this paper, a combination of NMR spectroscopic metabolic profiling of urine and statistical pattern recognition was used to detect focal inflammatory central nervous system (CNS) lesions induced by microinjection of a replication-deficient recombinant adenovirus expressing TNF-α or IL1-β cDNA into the brains of Wistar rats.
27 citations
TL;DR: A polyclonal antibody against calreticulin was used for the immunocytochemical localisation of the protein in Trypanosoma cruzi and significant differences in labelling were observed among the three evolutive forms of the protozoan.
Abstract: Calreticulin, a Ca2+ chaperone, is found in many different locations in various eukaryotic cells, including lumen of the endoplasmic reticulum, the cell surface, perinuclear areas and cytosolic granules. In the present study, a polyclonal antibody against calreticulin was used for the immunocytochemical localisation of the protein in Trypanosoma cruzi. Labelling was observed in the endoplasmic reticulum, Golgi complex, reservosomes, flagellar pocket, cell surface, cytosol, nucleus and kinetoplast. Significant differences in labelling were observed among the three evolutive forms of the protozoan. The functional role of calreticulin in T. cruzi is discussed.
24 citations
Patent•
18 Mar 2004TL;DR: In this article, the authors proposed methods for increasing the viscosity of xanthan include inducing particular key genes and increasing copy number of particular key key genes, which can be used to increase the visco-temporal properties of the Xanthan polymer.
Abstract: Increasing the molecular length of xanthan polymer makes a higher viscosity xanthan composition. Xanthan with higher specific viscosity characteristics provides more viscosity at equivalent concentration in food, industrial and oilfield applications. Methods for increasing the viscosity of xanthan include inducing particular key genes and increasing copy number of particular key genes.
12 citations
TL;DR: It was determined that when the StRGP reaction was carried out in the presence of UDP-[(14)C]Glc as the sugar donor and then 1 mM UDP was added in a chase-out experiment, radioactive UDP-Glc was obtained indicating that StR GP reaction seems to be reversible.
Abstract: Reversibly glycosylated polypeptides (RGPs) belong to a family of self-glycosylating proteins believed to be involved in plant polysaccharide synthesis. The precise function of these enzymes remains to be elucidated. Our results showed that the RGP 38-kDa subunit is phosphorylated in potato extracts (Solanum tuberosum L.). An increase in the self-glycosylation of Solanum tuberosum RGP (StRGP) 38-kDa subunit was observed after alkaline phosphatase (AP) treatment. Our results suggest that phosphorylation of StRGP appears to regulate its self-glycosylation. It was determined that when the StRGP reaction was carried out in the presence of UDP-[ 14 C]Glc as the sugar donor and then 1 mM UDP was added in a chase-out experiment, radioactive UDP-Glc was obtained indicating that StRGP reaction seems to be reversible. The anomeric configuration of transferred sugars to StRGP protein was also studied.
Journal Article•
TL;DR: This work determined that the p38 specific inhibitor reduces 50% NF-kappaB transcriptional activity, even in cells that overexpress distinct TIF2 deletions, and there is a physical interaction between Tif2 and p38 and RelA determined through in vitro translated protein binding assays.
Abstract: We have previously shown that nuclear receptor coactivator overexpression significantly enhanced NF-kappaB activity in a dose response manner. We studied the mechanism by which TIF2 regulates NF-kappaB activity. We determined that: 1) the p38 specific inhibitor reduces 50% NF-kappaB transcriptional activity, even in cells that overexpress distinct TIF2 deletions; 2) there is a physical interaction between TIF2 and p38 and RelA determined through in vitro translated protein binding assays; 3) TIF2 is a p38 substrate; 4) there is a physical interaction between TIF2 and IKK in TNF-alpha 20 ng/ml stimulated or not HEK 293 cell protein extract, and IkappaB only in basal conditions, determined by binding pull down assays. This NF-kappaB complex regulates its activity and targets gene expression in a determined physiologic context depending on the coactivator complex content.
TL;DR: LOV, an inhibitor of the hydroxy methylglutaryl CoA (HMGCoA) reductase, does not block LIF or OSM induced DNA replication and cell multiplication, and increasing concentrations of LOV block the mitogenic action of PGF(2alpha) by decreasing the number of cells capable of entering S-phase and dividing.
Patent•
18 Mar 2004TL;DR: In this article, it was found that a recombinant Xanthomonas campestris culture having multiple copies of gumB and gumC produces unpasteurized xanthan molecules having an intrinsic viscosity which is at least 20% greater than Xanthan from a corresponding strain of Xanthomanas Campestris not having multiple versions of gum B and gum C.
Abstract: A recombinant Xanthomonas campestris culture having multiple copies of gumB and gumC. It has been found that such a culture produces unpasteurized xanthan molecules having an intrinsic viscosity which is at least 20% greater than xanthan from a corresponding strain of Xanthomonas campestris not having multiple copies of gumB and gumC.
Patent•
18 Mar 2004TL;DR: In this paper, the authors proposed methods for increasing the viscosity of xanthan include inducing particular key genes and increasing copy number of particular key key genes, which can be used to increase the visco-temporal properties of the Xanthan polymer.
Abstract: Increasing the molecular length of xanthan polymer makes a higher viscosity xanthan composition. Xanthan with higher specific viscosity characteristics provides more viscosity at equivalent concentration in food, industrial and oilfield applications. Methods for increasing the viscosity of xanthan include inducing particular key genes and increasing copy number of particular key genes.