Fundación Instituto Leloir

About: Fundación Instituto Leloir is a facility organization based out in Buenos Aires, Argentina. It is known for research contribution in the topics: Dentate gyrus & Neurogenesis. The organization has 702 authors who have published 1052 publications receiving 39299 citations.

Dynamic RNA structures in the dengue virus genome.

Abstract: Dengue virus is an important human pathogen that belongs to the Flaviviridae family. The viral genome is a single molecule of RNA of positive polarity that plays multiple roles during the viral life cycle. Besides encoding the viral proteins, the genome contains RNA structures that regulate different viral processes. An important feature of dengue and other flavivirus genomes is the presence of inverted complementary sequences at the ends of the molecule that mediate long-range RNA-RNA interaction and genome cyclization. Recent studies have demonstrated that alternative conformations of the genome are necessary for infectivity. In this review, we discuss the current understanding of the function of different RNA elements that modulate dengue virus replication and provide new ideas of how dynamic RNA structures participate in the viral processes.

Light and temperature cues: multitasking receptors and transcriptional integrators.

Abstract: Contents Summary 1029 I. Introduction 1029 II. Convergence at the receptor 1030 III. Convergence at transcriptional hubs 1031 IV. Convergence involving clock components 1033 V. Conclusions 1033 Acknowledgements 1033 References 1033 SUMMARY: The combined information provided by light and temperature cues helps to optimise plant body architecture and physiology. Plants possess elaborate systems to sense and respond to these stimuli. Simultaneous perception of light and temperature by dual receptors such as phytochrome B and phototropin leads to immediate signalling convergence. Conversely, cue asynchronies initiate separate pathways and the information of the earliest cue is stored, awaiting the arrival of the later cue to control transcription. Storage mechanisms can involve changes in the activity of selected clock components or epigenetic modifications, depending on the time delay between cues (hours, days or several months). We propose a conceptual framework in which the mechanisms of integration relate to the timing of cue sensing.

A Polymeric Bacterial Protein Activates Dendritic Cells via TLR4

Abstract: The enzyme lumazine synthase from Brucella spp. (BLS) is a highly immunogenic protein that folds as a stable dimer of pentamers. It is possible to insert foreign peptides and proteins at the 10 N terminus of BLS without disrupting its general folding, and these chimeras are very efficient to elicit systemic and oral immunity without adjuvants. In this study, we show that BLS stimulates bone marrow dendritic cells from mice in vitro to up-regulate the levels of costimulatory molecules (CD40, CD80, and CD86) and major histocompatibility class II Ag. Furthermore, the mRNA levels of several chemokines are increased, and proinflammatory cytokine secretion is induced upon exposure to BLS. In vivo, BLS increases the number of dendritic cells and their expression of CD62L in the draining lymph node. All of the observed effects are dependent on TLR4, and clearly independent of LPS contamination. The described characteristics of BLS make this protein an excellent candidate for vaccine development.