Gadjah Mada University

About: Gadjah Mada University is a education organization based out in Yogyakarta, Indonesia. It is known for research contribution in the topics: Population & Adsorption. The organization has 17307 authors who have published 21389 publications receiving 116561 citations. The organization is also known as: University of Gajah Mada & Universitas Gadjah Mada.

Ability of curcuminoid compared to diclofenac sodium in reducing the secretion of cycloxygenase-2 enzyme by synovial fluid's monocytes of patients with osteoarthritis.

Abstract: AIM to assess the ability of curcuminoid from Curcuma domestica Val in reducing the cycloxygenase-2 secretion by synovial fluid's monocytes compared to diclofenac sodium in patients with osteoarthritis. METHODS this was a prospective randomized open end blinded evaluation (PROBE) study. The subjects were patients with knee osteoarthritis who were divided randomly into two groups, the first group received 30 mg 3 times daily of curcuminoid and the second group received 25 mg 3 times daily of diclofenac sodium. The joints aspiration was done and the secretion of cycloxygenase-2 enzyme by synovial fluid's monocytes was evaluated by scoring method before and after 4 weeks of treatments. RESULTS a total of 80 patients with knee osteoarthritis were enrolled. In curcuminoid group the average scores were 1.84±0.37 and 1.15±0.28 respectively (p<0.001). In diclofenac group the average scores were 1.79±0.38 and 1.12±0.27 respectively (p<0.001). In curcuminoid group the decreasing score of cycloxygenase-2 secretion was 0.70±0.51 while in diclofenac group was 0.67±0.45. There was no significant difference in decreasing the score of cycloxygenase enzyme secretion between both treatment groups (p=0.89). CONCLUSION the ability of curcuminoid from Curcuma domestica Val. rhizome extract was not significantly different compared to diclofenac sodium in suppressing the secretion of cycloxygenase-2 enzyme by synovial fluid's monocytes.

Identification of groundwater potential zones within an area with various geomorphological units by using several field parameters and a GIS approach in Kulon Progo Regency, Java, Indonesia

Abstract: A case study was conducted to find the groundwater potential zones in an area between the Serang and Bogowonto rivers, Kulon Progo Regency, Java, Indonesia. The objectives of this study were to delineate the groundwater potential zone based on a number of groundwater parameters that can be surveyed in the field and to incorporate the geomorphological conditions into these data. The geomorphology interpretation was conducted using the landform approach. This approach begins by preparing supporting data such as an Indonesian Topographic Map containing contour and land use data; a regional geology map containing lithology type and geology structures; and soil, climate, and hydrological data. The determination of the geomorphology unit was conducted manually by the visual interpretation of Digital Landsat ETM+ with some image interpretation keys. Four groundwater parameters were surveyed in the field: (a) depth to the water table, (b) water table fluctuation, (c) fluid electrical conductivity to represent groundwater quality, and (d) aquifer thickness. The groundwater potential zones were obtained by overlaying all the groundwater field parameters in terms of weighted overlay methods using the spatial analysis tool in ArcGIS 9.2. During the weighted overlay analysis, rankings were produced for each individual parameter of each groundwater field parameter, and weights were assigned based on the amount of influence they had (i.e., depth to the water table—30 %, water table fluctuation—20 %, aquifer thickness—30 %, and fluid conductivity—20 %). We then found the good, moderate, and poor zones in terms of groundwater potential, which had areas of 5.83, 4.53, and 2.36 km2, respectively. Areas with good groundwater potential are located largely within sand dunes, beach ridges, beaches, and fluviomarine plain landforms, which are characterized by a shallow water table, low fluctuation, thick aquifer, and low EC value. Moderate groundwater zones are generally characterized by poor water quality (high EC value), which is found to some degree in the alluvial plain. The regions with poor groundwater potential are spread mainly across the landforms composed of igneous rock (thin aquifers), such as denudational hills, which act as run-off zones due to their steep slope.

Protective effect of the isoflavone equol against DNA damage induced by ultraviolet radiation to hairless mouse skin

Abstract: Equol, an isoflavonoid metabolite produced from the dietary isoflavone daidzein by the gut microflora in mammals, has been found to protect not only against ultraviolet (UV) radiation-induced cutaneous inflammation and photoimmune suppression, but also have anti-photocarcinogenic properties in mice. Because the state of DNA damage has been correlated with suppression of the immune system and photocarcinogenesis, we have therefore examined the potential of equol to offer protection from solar-simulated UV (SSUV) radiation-induced DNA damage in hairless mice by the immunohistochemical approach using monoclonal antibody specific for cyclobutane pyrimidine dimers (CPDs; H3 antibody). Topical application of 20 µM equol lotion, which was applied both before and after SSUV significantly reduced the number of CPDs. This reduction was evident immediately after SSUV exposure, at 1 h after exposure, and at 24 h after exposure, revealing 54%, 50%, and 26% reduction in CPDs, respectively. When the same concentration was applied for 5 consecutive days after SSUV exposure, there was no significant difference in the reduction of CPDs immediately after SSUV irradiation or at 1 hour afterwards, but there were significant reductions of 23% and 42% at 24 and 48 h after SSUV exposure, respectively. Despite apparently reducing the number of CPDs post-SSUV, topically applied equol did not appear to increase the rate of dimer removal. To conclude, equol applied topically prior to SSUV irradiation offers protection against CPD formation in hairless mice, possibly by acting as a suncreen and thus inhibiting DNA photodamage.

Total Phenolic and Phytosterol Compounds and the Radical Scavenging Activity of Germinated Australian Sweet Lupin Flour

Abstract: In addition to their favourable nutritional profile, legumes also contain a range of bioactive compounds such as phenolic compounds and phytosterols which may protect against chronic diseases including cancer and cardiovascular disease. Germination of some legume seeds has been previously reported to increase the concentration of the bioactive compounds. In this study, the effect of germination of Australian Sweet Lupin (ASL) seeds for 9 days on the concentration of some bioactive compounds and the radical scavenging activity in the resulting flour was determined. The concentration of total phenolic compounds in methanolic extracts of germinated ASL flour was determined using Folin Ciocalteu reagent and phytosterols in oil extracts were analyzed by gas–liquid chromatography. The methanolic and oil extracts were also used to determine radical scavenging activity toward 2,2-diphenyl-1-picrylhydrazyl. In the methanolic extracts of germinated ASL flour, phenolic contents and the antioxidant activity were significantly increased following germination (700 and 1400 %, respectively). Analysis of the oil extracts of germinated ASL flour revealed that the concentration of phytosterols and the antioxidant activity were also increased significantly compared to ungerminated ASL flour (300 and 800 %, respectively). The relative proportion of phytosterols in germinated ASL flour was: β-sitosterol (60 %), stigmasterol (30 %) and campesterol (10 %). Germination increases the concentration of bioactive compounds and the radical scavenging activity in the germinated ASL flour.