Gadjah Mada University

About: Gadjah Mada University is a education organization based out in Yogyakarta, Indonesia. It is known for research contribution in the topics: Population & Adsorption. The organization has 17307 authors who have published 21389 publications receiving 116561 citations. The organization is also known as: University of Gajah Mada & Universitas Gadjah Mada.

Hypoadiponectinemia: a risk factor for metabolic syndrome.

Abstract: To find out correlation between plasma adiponectin levels, insulin resistance and IDF criteria of Mets Patients levels, insulin resistance and IDF criteria of Mets Patients. A case-control study was conducted on Native Javanese people from June 2006 to Januari 2007 in Outpatients Clinic of Dr. Sardjito Hospital. The case group involved patients aged between 20 to 55 years old. The diagnosis of metabolic syndrome was confirmed according to IDF criteria> Patients without metabolic syndrome with matching age and sex, were taken as control group. There were significant differences between case and control group for BMI (body mass index) (30.2+4.1 vs 26.9+4.7 kgm²), waist circumference (93.5+7.9 vs 84.4+ 11.5 cm), triglyceride (207.4+101.8 vs. 119.3+71.5 mg/dL), HDL cholesterol (48.6+9.4 vs.59.9+11.8 m g/dL, systolic blood pressure (132.8+17.9mmHg vs 120.6+13.5 mmHg) and diastolic blood pressure (83.8+40.8 mg/dL vs. 100.7+29.4 mg/dL), HOMA index (6.7+17.4 vs.20+ 2.0) and adiponectin levels (3.8 +1.4 vs.5.9+2.5), respectively. For metabolic syndrome, hypoadionectinemia showed the OR value of 6.0 (95%CI 1.3 to 25.02), after adjustment for waist circumference, TG.HLD, blood pressure, fasting blood glucose. Hypoadiponectinemia and insulin resistance represent independent risk factors for metabolic syndrome development.

Characterization of the tufB-secE-nusG-rplKAJL-rpoB Gene Cluster of the Citrus Greening Organism and Detection by Loop-Mediated Isothermal Amplification

Abstract: Okuda, M., Matsumoto, M., Tanaka, Y., Subandiyah, S., and Iwanami, T. 2005. Characterization of the tufB-secE-nusG-rplKAJL-rpoB gene cluster of the citrus greening organism and detection by loop-mediated isothermal amplification. Plant Dis. 89:705-711. Thermal asymmetric interlaced polymerase chain reaction (TAIL-PCR) was performed to amplify the uncharacterized regions adjacent to the nusG-rplKAJL-rpoB gene cluster of citrus greening organism (GO) isolates from different locations in Japan and Indonesia. Conventional PCR was used to amplify the internal nusG-rplKAJL-rpoB gene cluster of these isolates, and the complete sequence of this 6.1-kb fragment was determined. Comparisons with other bacterial sequences showed that the fragment is the tufB-secE-nusG-rplKAJL-rpoB gene cluster. The organization of this gene cluster is similar to that of the homologous cluster found in Escherichia coli. Except for three nucleotide changes, the sequence was identical among Japanese and Indonesian isolates. A loop-mediated isothermal amplification (LAMP) assay based on the conserved sequence of the nusG-rplKAJL-rpoB gene cluster was developed for the detection of the GO. The LAMP product was rapidly detected on nylon membranes by staining with AzurB. LAMP could detect as low as about 300 copies of the nusG-rplKAJL-rpoB fragment of the Japanese and Indonesian isolates of GO. The LAMP-based detection method, which does not depend upon a thermal cycler and electrophoresis apparatus, will be useful for under-equipped laboratories, including those found in extension centers and quarantine offices. Additional keywords: Diaphorina citri, quantitative PCR