Showing papers by "Georgia College & State University published in 2020"

Understanding financial auditing from a service perspective

Abstract: This paper draws from the service science and professional service literatures to conceptualize financial auditing as an economic service. A central characteristic of economic services is the participation of the customer/client in the production process. The necessity of having the customer/client be a co-producer introduces greater heterogeneity to the provision of services relative to the manufacturing of goods which, in turn, creates a tension between service quality and service efficiency. One implication of this tension is that standardization of the audit process may not increase audit quality. We further argue that audit research should give more attention to the idiosyncratic nature of audit engagements and the importance of successful cooperation between the service provider (the audit firm) and the client for improving audit quality. Utilizing research on service networks, we draw attention to a broader perspective than the dyadic relations of service provider and client to show that the possible frictions between the value of co-creation of the service and the independence of the service professional are endemic to the service process, implying that efforts to maximize auditor independence may have unexpected costs that impair audit quality.

Structural Insight into the Mechanism of N-Linked Glycosylation by Oligosaccharyltransferase

Abstract: Asparagine-linked glycosylation, also known as N-linked glycosylation is an essential and highly conserved post-translational protein modification that occurs in all three domains of life. This modification is essential for specific molecular recognition, protein folding, sorting in the endoplasmic reticulum, cell–cell communication, and stability. Defects in N-linked glycosylation results in a class of inherited diseases known as congenital disorders of glycosylation (CDG). N-linked glycosylation occurs in the endoplasmic reticulum (ER) lumen by a membrane associated enzyme complex called the oligosaccharyltransferase (OST). In the central step of this reaction, an oligosaccharide group is transferred from a lipid-linked dolichol pyrophosphate donor to the acceptor substrate, the side chain of a specific asparagine residue of a newly synthesized protein. The prokaryotic OST enzyme consists of a single polypeptide chain, also known as single subunit OST or ssOST. In contrast, the eukaryotic OST is a complex of multiple non-identical subunits. In this review, we will discuss the biochemical and structural characterization of the prokaryotic, yeast, and mammalian OST enzymes. This review explains the most recent high-resolution structures of OST determined thus far and the mechanistic implication of N-linked glycosylation throughout all domains of life. It has been shown that the ssOST enzyme, AglB protein of the archaeon Archaeoglobus fulgidus, and the PglB protein of the bacterium Campylobactor lari are structurally and functionally similar to the catalytic Stt3 subunit of the eukaryotic OST enzyme complex. Yeast OST enzyme complex contains a single Stt3 subunit, whereas the human OST complex is formed with either STT3A or STT3B, two paralogues of Stt3. Both human OST complexes, OST-A (with STT3A) and OST-B (containing STT3B), are involved in the N-linked glycosylation of proteins in the ER. The cryo-EM structures of both human OST-A and OST-B complexes were reported recently. An acceptor peptide and a donor substrate (dolichylphosphate) were observed to be bound to the OST-B complex whereas only dolichylphosphate was bound to the OST-A complex suggesting disparate affinities of two OST complexes for the acceptor substrates. However, we still lack an understanding of the independent role of each eukaryotic OST subunit in N-linked glycosylation or in the stabilization of the enzyme complex. Discerning the role of each subunit through structure and function studies will potentially reveal the mechanistic details of N-linked glycosylation in higher organisms. Thus, getting an insight into the requirement of multiple non-identical subunits in the N-linked glycosylation process in eukaryotes poses an important future goal.

Logistic Optimization for Multi Depots Loading Capacitated Electric Vehicle Routing Problem From Low Carbon Perspective

Abstract: In this paper, a multi depots capacitated electric vehicle routing problem where client demand is composed of two-dimensional weighted items (2L-MDEVRP) is addressed. This problem calls for the minimization of the transportation distance required for the delivery of the items which are demanded by the clients, carried out by a fleet of electric vehicles in several depots. Since the 2L-MDEVRP is an NP-hard problem, a heuristic algorithm combined variable neighborhood search algorithm (VNS) and space saving heuristic algorithm (SSH) is proposed. The VNS algorithm is used to solve the vehicle routing problem (VRP) sub-problem, and the SSH algorithm is used to solve the bin packing problem (BPP) sub-problem. We propose the space saving heuristic to find the best matching solution between the next loading item and the feasible loading position. The SSH-VNS algorithm is tested by using benchmark instances available from the literature. The results show that the SSH-VNS algorithm has better performance compared with other published results for solving capacity vehicle routing problem (CVRP) and two-dimensional capacity vehicle routing problem (2L-CVRP). Some new best-known solutions of the benchmark problem are also found by SSH-VNS. Moreover, the effectiveness of the proposed algorithm on 2L-MDEVRP is demonstrated through numerical experiments and a practical logistic distribution case. In the last section, the managerial implications and suggestions for future research are also discussed.

Testing the viability of emotions and issue involvement as predictors of CSA response behaviors

Abstract: Corporate social advocacy (CSA) has become more prominent as companies continue taking stands on politically charged social issues. This study examines emotions and issue involvement as antecedents...

Young Children's Theory of Mind Predicts More Sharing With Friends Over Time.

Abstract: Children (predominantly white and middle class) between 3 and 6 years (M = 55.12 months, N = 145 at Time 1, N = 102 at Time 2) participated in the prosocial choice test at two time points approximately 10 months apart. Children could share with strangers, close friends, nonfriends, and in a control, no recipient condition. Children shared more rewards with friends over time. Age interacted with recipient type such that older children had a higher probability of prosocial allocations toward friends and strangers compared to younger children. Theory of mind (ToM) predicted more prosocial allocations to friends over time, and the youngest children with higher ToM scores showed the largest increase in sharing with friends over time. [ABSTRACT FROM AUTHOR]

The marijuana user in US news media: an examination of visual stereotypes of race, culture, criminality and normification:

Abstract: In the wake of growing legalization efforts, both medicinal and recreational marijuana use in the US is becoming more prevalent and societally acceptable. However, racial, criminal and cultural ste...

Chandra Observations of High-energy X-Ray Sources Discovered by INTEGRAL

Abstract: The International Gamma-Ray Astrophysics Laboratory (INTEGRAL) satellite has detected in excess of 1000 sources in the ~20-100 keV band during its surveys of the sky over the past 17 years. We obtained 5 ks observations of 15 unclassified IGR sources with the Chandra X-ray Observatory in order to localize them, to identify optical/IR counterparts, to measure their soft X-ray spectra, and to classify them. For 10 of the IGR sources, we detect Chandra sources that are likely (or in some cases certain) to be the counterparts. IGR J18007-4146 and IGR J15038-6021 both have Gaia parallax distances, placing them at 2.5+0.5-0.4 and 1.1+1.5-0.4 kpc, respectively. We tentatively classify both of them as intermediate polar-type Cataclysmic Variables. Also, IGR J17508-3219 is likely to be a Galactic source, but it is unclear if it is a Dwarf Nova or another type of transient. For IGR J17118-3155, we provide a Chandra localization, but it is unclear if the source is Galactic or extragalactic. Based on either near-IR/IR colors or the presence of extended near-IR emission, we classify four sources as Active Galactic Nuclei (IGR J16181-5407, IGR J16246-4556, IGR J17096-2527, and IGR J19294+1327), and IGR J20310+3835 and IGR J15541-5613 are AGN candidates. In addition, we identified an AGN in the INTEGRAL error circle of IGR J16120-3543 that is a possible counterpart.

Degradation of Transcriptional Repressor ATF4 during Long-Term Synaptic Plasticity

Abstract: Maintenance of long-term synaptic plasticity requires gene expression mediated by cAMP-responsive element binding protein (CREB). Gene expression driven by CREB can commence only if the inhibition by a transcriptional repressor activating transcription factor 4 (ATF4; also known as CREB2) is relieved. Previous research showed that the removal of ATF4 occurs through ubiquitin-proteasome-mediated proteolysis. Using chemically induced hippocampal long-term potentiation (cLTP) as a model system, we investigate the mechanisms that control ATF4 degradation. We observed that ATF4 phosphorylated at serine-219 increases upon induction of cLTP and decreases about 30 min thereafter. Proteasome inhibitor β-lactone prevents the decrease in ATF4. We found that the phosphorylation of ATF4 is mediated by cAMP-dependent protein kinase. Our initial experiments towards the identification of the ligase that mediates ubiquitination of ATF4 revealed a possible role for β-transducin repeat containing protein (β-TrCP). Regulation of ATF4 degradation is likely to be a mechanism for determining the threshold for gene expression underlying maintenance of long-term synaptic plasticity and by extension, long-term memory.

Suffering in Silence: How COVID-19 School Closures Inhibit the Reporting of Child Maltreatment

Abstract: To combat the spread of COVID-19, many primary and secondary schools in the United States canceled classes and moved instruction online. This study examines an unexplored consequence of COVID-19 school closures: the broken link between child maltreatment victims and the number one source of reported maltreatment allegations---school personnel. Using current, county-level data from Florida, we estimate a counterfactual distribution of child maltreatment allegations for March and April 2020, the first two months in which Florida schools closed. While one would expect the financial, mental, and physical stress due to COVID-19 to result in additional child maltreatment cases, we find that the actual number of reported allegations was approximately 15,000 lower (27 percent) than expected for these two months. We leverage a detailed dataset of school district staffing and spending to show that the observed decline in allegations was largely driven by school closures. Finally, we discuss policy implications of our findings for the debate surrounding school reopenings and suggest a number of responses that may mitigate this hidden cost of school closures.

New and interesting aerial diatom assemblages from southwestern Iceland

Abstract: Examination of algal assemblages from aerial environments around the globe, especially those from pseudoaerial habitats found on moistened rocks underneath waterfalls or around springs and seeps, reveals the presence of unique diatom floras. Yet, diatom assemblages from northern regions like Iceland remain understudied, especially those from the volcanic rock outcrops and boulders that create euaerial habitats where biota receive moisture from the atmosphere or the rock itself. During the summers of 2013 and 2015, we examined the biodiversity of mostly euaerial, but also pseudoaerial, diatom assemblages collected from volcanic rock outcrops or large boulders on the landscape from southwestern Iceland. We used light and scanning electron microscopy to document the biodiversity of common, smaller, new, or interesting specimens, such as Humidophila and Eunotia. We describe one new Humidophila species, H. eldfjallii sp. nov., with triundulate valve margins and include information on another unidentified taxon, Humidophila sp. 1, naviculoid in shape with tapering to rounded ends, continuous striae through the length of the valve, and a circular central area. We formally transfer Diadesmis contenta var. biceps to Humidophila biceps. To correct the nomenclature, we recognized Humidophila parallela at the species level. Relative abundance estimates of diatom populations provided further characterization of the assemblages on these habitats. Humidophila taxa, especially H. gallica dominated the diverse diatom flora. We discuss adaptations for survival with access to mostly atmospheric water. The diatom flora described here adds to the flora for this region, highlights the diversity of diatom assemblages that can inhabit euaerial environments, and provides evidence of adaptive success of diatoms in extreme habitats with limited moisture and nutrients.

Online social viewing: Cross-cultural adoption and uses of bullet-screen videos

Abstract: Bullet-screen technology, an innovative way of interacting with online videos, allows viewers to contribute comments that simultaneously appear over videos. Popular in East Asia, the technology is ...

Evolution of MAXI J1631-479 during the January 2019 outburst observed by INTEGRAL/IBIS

Abstract: We report on a recent bright outburst from the new X-ray binary transient MAXIJ1631-479, observed in January 2019. In particular, we present the 30-200 keV analysis of spectral transitions observed with INTEGRAL/IBIS during its Galactic Plane monitoring program. In the MAXI and BAT monitoring period, we observed two different spectral transitions between the high/soft and low/hard states. The INTEGRAL spectrum from data taken soon before the second transition, is best described by a Comptonised thermal component with a temperature of kTe ~ 30 keV and a high luminosity value of L2-200 keV ~ 3×1038 erg s-1 (assuming a distance of 8 kpc). During the second transition, the source shows a hard, power-law spectrum. The lack of high energy cut-off indicates that the hard X-ray spectrum from MAXI J1631-479 is due to a non-thermal emission. Inverse Compton scattering of soft X-ray photons from a non-thermal or hybrid thermal/non-thermal electron distribution can explain the observed X-ray spectrum although a contribution to the hard X-ray emission from a jet cannot be determined at this stage. The outburst evolution in the hardness-intensity diagram, the spectral characteristics and the rise and decay times of the outburst are suggesting this system is a black hole candidate.

The utility of the Psychopathy Checklist‐Revised (PCL‐R) facet and item scores in predicting violent recidivism

Abstract: There is relatively limited research on psychopathy in non-Caucasian ethnic groups and even less on the utility of the Psychopathy Checklist-Revised (PCL-R) that focuses on PCL-R facet and item scores in predicting violent recidivism. In this study, we assessed the utility of the PCL-R in prospectively predicting violent versus nonviolent recidivism during an 11-year follow-up window. A high-risk sample of 451 incarcerated Korean male offenders was assessed on the PCL-R at baseline. A total of 445 were reconvicted after release (353 violent and 92 nonviolent recidivists). Psychopathy facet scores were higher in violent compared to nonviolent recidivists. Facet 2 (affective) showed the strongest effect size (Cohen's d = 0.53; Percentage change in odds = 22.6%) in predicting violent recidivism. Analyses of the four items constituting the affective facet indicated that callous/lack of empathy (Percentage change in odds = 134.4%) and failure to accept responsibility (Percentage change in odds = 94.5%) were the strongest predictors of violent recidivism. Findings are to our knowledge the first to document the utility of the PCL-R in distinguishing violent from nonviolent recidivism and highlight the role of affective impairment (particularly lack of empathy) in violent recidivism.

A study of n-Lie-isoclinic Leibniz algebras

Abstract: In this paper, we introduce the concept of n-Lie-isoclinism on non-Lie Leibniz algebras. Among the results obtained, we provide several characterizations of n-Lie-isoclinic classes of Leibniz algeb...

Blow-Up Results for Space-Time Fractional Stochastic Partial Differential Equations

Abstract: Consider non-linear time-fractional stochastic reaction-diffusion equations of the following type, $$ \partial^{\beta}_{t}u_{t}(x)=- u(-{\Delta})^{\alpha/2} u_{t}(x)+I^{1-\beta}[b(u)+ \sigma(u)\stackrel{\cdot}{F}(t,x)] $$ in (d + 1) dimensions, where ν > 0,β ∈ (0, 1), α ∈ (0, 2]. The operator $\partial ^{\beta }_{t}$ is the Caputo fractional derivative while − (−Δ)α/2 is the generator of an isotropic α-stable Levy process and I1−β is the Riesz fractional integral operator. The forcing noise denoted by $\stackrel {\cdot }{F}(t,x)$ is a Gaussian noise. These equations might be used as a model for materials with random thermal memory. We derive non-existence (blow-up) of global random field solutions under some additional conditions, most notably on b, σ and the initial condition. Our results complement those of P. Chow in (Commun. Stoch. Anal. 3(2):211–222, 2009), Chow (J. Differential Equations 250(5):2567–2580, 2011), and Foondun et al. in (2016), Foondun and Parshad (Proc. Amer. Math. Soc. 143(9):4085–4094, 2015) among others.

Critical parameters for reaction–diffusion equations involving space–time fractional derivatives

Abstract: We will look at reaction–diffusion type equations of the following type, $$\begin{aligned} \partial ^\beta _tV(t,x)=-(-\Delta )^{\alpha /2} V(t,x)+I^{1-\beta }_t[V(t,x)^{1+\eta }]. \end{aligned}$$ We first study the equation on the whole space by making sense of it via an integral equation. Roughly speaking, we will show that when $$0<\eta \leqslant \eta _c$$ , there is no global solution other than the trivial one while for $$\eta >\eta _c$$ , non-trivial global solutions do exist. The critical parameter $$\eta _c$$ is shown to be $$\frac{1}{\eta ^*}$$ where $$\begin{aligned} \eta ^*:=\sup _{a>0}\left\{ \sup _{t\in (0,\,\infty ),x\in \mathbb {R}^d}t^a\int _{\mathbb {R}^d}G(t,\,x-y)V_0(y)\,\mathrm{d}y<\infty \right\} \end{aligned}$$ and $$G(t,\,x)$$ is the heat kernel of the corresponding unforced operator. $$V_0$$ is a non-negative initial function. We also study the equation on a bounded domain with Dirichlet boundary condition and show that the presence of the fractional time derivative induces a significant change in the behavior of the solution.

Phenotypic Comparability from Genotypic Variability among Physically Structured Microbial Consortia

Abstract: Microbiomes represent the collective bacteria, archaea, protist, fungi, and virus communities living in or on individual organisms that are typically multicellular eukaryotes. Such consortia have become recognized as having significant impacts on the development, health, and disease status of their hosts. Since understanding the mechanistic connections between an individual's genetic makeup and their complete set of traits (i.e., genome to phenome) requires consideration at different levels of biological organization, this should include interactions with, and the organization of, microbial consortia. To understand microbial consortia organization, we elucidated the genetic constituents among phenotypically similar (and hypothesized functionally-analogous) layers (i.e., top orange, second orange, pink, and green layers) in the unique laminated orange cyanobacterial-bacterial crusts endemic to Hawaii's anchialine ecosystem. High-throughput amplicon sequencing of ribosomal RNA hypervariable regions (i.e., Bacteria-specific V6 and Eukarya-biased V9) revealed microbial richness increasing by crust layer depth, with samples of a given layer more similar to different layers from the same geographic site than to their phenotypically-analogous layer from different sites. Furthermore, samples from sites on the same island were more similar to each other, regardless of which layer they originated from, than to analogous layers from another island. However, cyanobacterial and algal taxa were abundant in all surface and bottom layers, with anaerobic and chemoautotrophic taxa concentrated in the middle two layers, suggesting crust oxygenation from both above and below. Thus, the arrangement of oxygenated vs. anoxygenated niches in these orange crusts is functionally distinct relative to other laminated cyanobacterial-bacterial communities examined to date, with convergent evolution due to similar environmental conditions a likely driver for these phenotypically comparable but genetically distinct microbial consortia.

Toward developing an applied framework to help foster success in generational transition of family businesses

Abstract: Family business is a critical component of most economies worldwide as a majority of businesses are either owned or managed by family members. The transfer of ownership across generations that will...

Ideal cardiovascular health in urban Jamaica: prevalence estimates and relationship to community property value, household assets and educational attainment: a cross-sectional study.

Abstract: Objective Ideal cardiovascular health (ICH) is associated with greater longevity and reduced morbidity, but no research on ICH has been conducted in Jamaica. We aimed to estimate the prevalence of ICH in urban Jamaica and to evaluate associations between ICH and community, household, and individual socioeconomic status (SES). Design Cross-sectional study. Setting Urban communities in Jamaica. Participants 360 men and 665 women who were urban residents aged ≥20 years from a national survey, the Jamaica Health and Lifestyle Survey 2016–2017. Exposures Community SES, using median land values (MLV); household SES, using number of household assets; and individual SES, using education level. Primary outcome The main outcome variable was ICH, defined as having five or more of seven ICH characteristics (ICH-5): current non-smoking, healthy diet, moderate physical activity, normal body mass index, normal blood pressure, normal glucose and normal cholesterol. Prevalence was estimated using weighted survey design and logistic regression models were used to evaluate associations. Results The prevalence of overall ICH (seven characteristics) was 0.51%, while the prevalence of ICH-5 was 22.9% (male 24.5%, female 21.5%, p=0.447). In sex-specific multivariable models adjusted for age, education, and household assets, men in the lower tertiles of community MLV had lower odds of ICH-5 compared with men in the upper tertile (lowest tertile: OR 0.33, 95% CI 0.12 to 0.91, p=0.032; middle tertile: OR 0.46, 95% CI 0.20 to 1.04, p=0.062). Women from communities in the lower and middle tertiles of MLV also had lower odds of ICH-5, but the association was not statistically significant. Educational attainment was inversely associated with ICH-5 among men and positively associated among women. Conclusion Living in poorer communities was associated with lower odds of ICH-5 among men in Jamaica. The association between education level and ICH-5 differed in men and women.

Measuring What Students Know: SNAP’s Guidelines and Suggestions for Assessing Goal 1 Content in Psychology

Abstract: Although many psychology departments and instructors are aware of the American Psychological Association Guidelines for the Undergraduate Psychology Major Version 2.0, they are often less aware of ...

Improving Representation of Underrepresented Minority (URM) Students in Oncology Biomedical Research Workforce: Outcome Evaluation from the ReTOOL Program.

Abstract: The Research Training Opportunities for Outstanding Leaders (ReTOOL) program was implemented in 2012 to increase the representation of racial and ethnic minorities in the biomedical workforce. Specifically, the ReTOOL program aims to foster the capacity for scientific research among underserved populations as well as address the cultural appropriateness of research projects. This paper describes the impact of the ReTOOL program in enhancing the research training of underrepresented minority (URM) students. Forty URM students who completed the ReTOOL program between 2012 and 2019 were invited to participate in the program evaluation. The response rate was 73% with 29 participants. Of the 29 participants, 26 trainees self-identified as Black or African-American. A structured survey developed for the program was employed for data collection, using a Likert Scale ranging from 1 to 5, with 5 being the best. The item ratings ranged from 4.45 to 4.80. Responses to open-ended questions show that ReTOOL has been instrumental in socializing and acculturating participants into the habits of scientific thinking. The combined use of quantitative and qualitative inquiry depicts that ReTOOL has been highly successful in fostering participant enrollment in advanced health-related or professional degree programs.

X-ray spectral and flux variability of the microquasar GRS 1758-258 on timescales from weeks to years

Abstract: We present the spectral and timing evolution of the persistent black hole X-ray binary GRS 1758−258 based on almost 12 years of observations using the Rossi X-ray Timing Explorer Proportional Counter Array. While the source was predominantly found in the hard state during this time, it entered the thermally dominated soft state seven times. In the soft state GRS 1758−258 shows a strong decline in flux above 3 keV rather than the pivoting flux around 10 keV more commonly shown by black hole transients. In its 3–20 keV hardness intensity diagram, GRS 1758−258 shows a hysteresis of hard and soft state fluxes typical for transient sources in outburst. The RXTE-PCA and RXTE-ASM long-term light curves do not show any orbital modulations in the range of 2–30 d. However, in the dynamic power spectra significant peaks drift between 18.47 and 18.04 d for the PCA data, while less significant signatures between 19 d and 20 d are seen for the ASM data as well as for the Swift /BAT data. We discuss different models for the hysteresis behavior during state transitions as well as possibilities for the origin of the long term variation in the context of a warped accretion disk.

Interpersonal and Affective Facets and Items of the Psychopathy Checklist-Revised (PCL-R) in Predicting Child Sex Offending**An earlier version of this study was presented at 2019 Annual Meeting of the American Society of Criminology.:

Abstract: Psychopathy of child sex offenders in non-Western and Asian population is not frequently reported. The study examined psychopathic traits assessed by the Psychopathy Checklist-Revised (PCL-R) in th...

Identifying the primary sources of fecal contamination along the beaches and rivers of Trinidad.

Abstract: The aim of this study was to identify the main sources of fecal pollution at popular beaches and rivers in the island of Trinidad. Escherichia coli enumeration and microbial source tracking (MST) were used to identify the primary sources of fecal bacteria contamination at the sites. Nineteen sites exceeded USEPA water quality standards for safe recreational use. Highest levels of fecal contamination were recorded on the central and west coasts of the island and included Brickfield River (4,839 MPN 100 ml-1), Orange Valley Bay (2,406.6 MPN 100 ml-1) and Chaguaramas Bay (1,921.2 MPN 100 ml-1). MST detected human (HF183) fecal pollution at ∼63%, birds at ∼67%, chicken at ∼36% and cattle (BacCow) at ∼34% of the sites. MST is a useful and rapid method for identifying major sources of fecal pollution in rivers and beaches. In Trinidad water bodies, the main sources of fecal pollution were humans and birds. The large number of sites with elevated levels of fecal pollution detected is particularly alarming and represents a serious public health risk.

Chloroplast mRNAs are 3' polyuridylylated in the Green Alga Pithophora roettleri (Cladophorales).

Abstract: Species within the green algal order Cladophorales have an unconventional plastome structure where individual coding regions or small numbers of genes occur as linear single-stranded DNAs folded into hairpin structures. Another group of photosynthetic organisms with an equivalently reduced chloroplast genome are the peridinin dinoflagellates of the Alveolata eukaryotic lineage whose plastomes are mini-circles carrying one or a few genes required for photosynthesis. One unusual aspect of the Alveolata is the polyuridylylation of mRNA 3' ends among peridinin dinoflagellates and the chromerid algae. This study was conducted to understand if an unconventional highly reduced plastome structure co-occurs with unconventional RNA processing. To address this, the 5' and 3' mRNA termini of the known chloroplast genes of Pithophora roettleri (order Cladophorales) were analyzed for evidence of post-transcriptional processing. Circular Reverse Transcriptase PCR (cRT-PCR) followed by deep sequencing of the amplicons was used to analyze 5' and 3' mRNA termini. Evidence of several processing events were collected, most notably the 3' termini of six of the eight genes were polyuridylylated, which has not been reported for any lineage outside of the Alveolata. Other processing events include poly(A) and heteropolymeric 3' additions, 5' primary transcript start sites, as well as the presence of circularized RNAs. Five other species representing other green algal lineages were also tested and poly(U) additions appear to be limited to the order Cladophorales. These results demonstrate that chloroplast mRNA polyuridylylation is not the sole provenance of photosynthetic alveolates and may have convergently evolved in two distinct photosynthetic lineages.