Showing papers by "Gifu University of Medical Science published in 2018"

Microtubule polyglutamylation and acetylation drive microtubule dynamics critical for platelet formation

Abstract: Upon maturation in the bone marrow, polyploid megakaryocytes elongate very long and thin cytoplasmic branches called proplatelets. Proplatelets enter the sinusoids blood vessels in which platelets are ultimately released. Microtubule dynamics, bundling, sliding, and coiling, drive these dramatic morphological changes whose regulation remains poorly understood. Microtubule properties are defined by tubulin isotype composition and post-translational modification patterns. It remains unknown whether microtubule post-translational modifications occur in proplatelets and if so, whether they contribute to platelet formation.

Existing Approaches to Chemical, Biological, Radiological, and Nuclear (CBRN) Education and Training for Health Professionals: Findings from an Integrative Literature Review.

Abstract: Objectives This review was conducted to explore the literature to determine the availability, content, and evaluation of existing chemical, biological, radiological, and nuclear (CBRN) education programs for health professionals. Methods An integrative review of the international literature describing disaster education for CBRN (2004-2016) was conducted. The following relevant databases were searched: Proquest, Pubmed, Science Direct, Scopus, Journals @ OVID, Google Scholar, Medline, and Ichuschi ver. 5 (Japanese database for health professionals). The search terms used were: “disaster,” “chemical,” “biological,” “radiological,” “nuclear,” “CBRN,” “health professional education,” and “method.” The following Medical Subject Headings (MeSH) terms, “education,” “nursing,” “continuing,” “disasters,” “disaster planning,” and “bioterrorism,” were used wherever possible and appropriate. The retrieved articles were narratively analyzed according to availability, content, and method. The content was thematically analyzed to provide an overview of the core content of the training. Results The literature search identified 619 potentially relevant articles for this study. Duplicates (n=104) were removed and 87 articles were identified for title review. In total, 67 articles were discarded, yielding 20 articles for all-text review, following 11 studies were retained for analysis, including one Japanese study. All articles published in English were from the USA, apart from the two studies located in Japan and Sweden. The most typical content in the selected literature was CBRN theory (n=11), followed by studies based on incident command (n=8), decontamination (n=7), disaster management (n=7), triage (n=7), personal protective equipment (PPE) use (n = 5), and post-training briefing (n=3). Conclusion While the CBRN training course requires the participants to gain specific skills and knowledge, proposed training courses should be effectively constructed to include approaches such as scenario-based simulations, depending on the participants’ needs. Kako M , Hammad K , Mitani S , Arbon P . Existing Approaches to Chemical, Biological, Radiological, and Nuclear (CBRN) Education and Training for Health Professionals: Findings from an Integrative Literature Review. Prehosp Disaster Med. 2018;33(2):182–190.

A novel CYCS mutation in the α-helix of the CYCS C-terminal domain causes non-syndromic thrombocytopenia.

Abstract: We report a patient with thrombocytopenia from a Japanese family with hemophilia A spanning four generations. Various etiologies of thrombocytopenia, including genetic, immunological, and hematopoietic abnormalities, determine the prognosis for this disease. In this study, we identified a novel heterozygous mutation in a gene encoding cytochrome c, somatic (CYCS, MIM123970) using whole exome sequencing. This variant (c.301_303del:p.Lys101del) is located in the α-helix of the cytochrome c (CYCS) C-terminal domain. In silico structural analysis suggested that this mutation results in protein folding instability. CYCS is one of the key factors regulating the intrinsic apoptotic pathway and the mitochondrial respiratory chain. Using the yeast model system, we clearly demonstrated that this one amino acid deletion (in-frame) resulted in significantly reduced cytochrome c protein expression and functional defects in the mitochondrial respiratory chain, indicating that the loss of function of cytochrome c underlies thrombocytopenia. The clinical features of known CYCS variants have been reported to be confined to mild or asymptomatic thrombocytopenia, as was observed for the patient in our study. This study clearly demonstrates that thrombocytopenia can result from CYCS loss-of-function variants.

Effect of Controlled Consciousness on Sense of Presence and Visually Induced Motion Sickness While Viewing Stereoscopic Movies

Abstract: In our previous study, we found it is possible to have an effect of change in the condition of consciousness (allocation of consciousness) on visually evoked postural response (VEPRs) Then, in this study, we verified effect of controlling consciousness on VEPRs, visually induced motion sickness (VIMS), a sense of presence while viewing 3D movies. Participants watched 3D movie consisted of several colored balls sinusoidal moving at 0.25 Hz in the depth direction for 3 min each under condition following pre-instruction. The detail of the pre-instruction were “sway body in a parallel/opposite direction”. The position of the body sway center of pressure was continuously recorded. As subjective evaluation, participants completed a simulator sickness questionnaire and reported three feelings (the sense of presence, motion and interactive) by using a visual analog scale. The results clearly showed that (1) The influence of pre-instruction appeared much stronger than that of VEPRs, and (2) The relationship between change in body sway and degree of VIMS or the feeling of presence do not always match under the situation included multi factors related to sense information or the condition of consciousness.

Evaluation of Tumor Tissue Fixation Effects of Formulation Modified Mohs Pastes in Mice and Their Water-Absorbing Properties.

Abstract: Mohs paste (MP) is a hospital preparation containing zinc hydrochloride and zinc oxide starch. It is a topical medication used to fixate tissues for the removal of inoperable skin tumors and the management of hemorrhage and exudates, and to prevent foul odor resulting from secondary infections. However, it has problems, such as changes in hardness and viscoelasticity with time and liquefaction by exudate. It has been reported that the modified MP with D-sorbitol (S-MP) and the modified MP using the cellulose instead of starch (C-MP) have excellent physicochemical stability and better handling than original MP (O-MP). In this study, the effect of prescription improvement of MP on the pharmacological effect was examined with reference to water absorbing property, and its tumor tissue invasion fixation depth as an indicator. In the S-MP and C-MP, the amounts of water absorption did not differ significantly from those in the O-MP. The hardness of S-MP was decreased and liquefied like O-MP after absorbing water. In contrast, C-MP retained its form even after water absorption. The subcutaneous tumors in mice treated with modified MP formulations were measured for invasion fixation depth at 6 and 24 h after application. And the tissue status was observed using computed tomography. In all MPs, invasion fixation depth increased depending on application time. S-MP and O-MP depths did not differ significantly. The invasion depths of the C-MP significantly increased compared with those in the O-MP. These results suggest that C-MP had a high tissue fixation rate.

The Impact of 3D Games on Brain Blood Flow Dynamics and its Utilization

Abstract: A 3D game machine/video clip is focused on to enhance the brain activity. Virtual space might enhance the brain activity for the mild cognitive impairment (MCI) that are now reported to increase in the aging society. We herein increase knowledge about the influence of stereoscopic images on the body, especially on the brain activity. The electroencephalograms (EEG) and the cerebral blood flow are measured in this study. Functional near-infrared imaging (fNIRS) was used to determine if either 2D or 3D video games changes brain activity.

Anatomo-functional basis of restless legs syndrome

Abstract: Restless legs syndrome (RLS) is a common sensory-motor disorder characterized by a variety of uncomfortable sensations associated with an urge to move the legs, relief with movement, strong circadian modulation, and often occurring at sleep onset, impairing sleep quality and inducing insomnia and reduced quality of life. RLS often responds to dopaminergic treatment, but over time, augmentation develops. Some additional key features of the disorder include greater somatotrophic spread when severe or with augmentation, painful sensations, and an association with brain iron deficiency. These features across neurophysiologic subsystems suggest a neural network disorder.

Examining Japanese women's preferences for a new style of postnatal care facility and its attributes.

Abstract: Perinatal care in rural Japan is currently facing a crisis because of the lack of medical staff, especially obstetricians. In this study, a new style of postnatal care facility that combines both medical and nonmedical support is considered. Contrary to most postnatal care facilities in Japan, this new postnatal care facility accepts a puerperant from the cooperating maternity facility soon after birth (≤2 days). We conducted a hypothetical choice experiment to investigate whether this new postnatal care facility could be accepted by women in Gero City, Hida, Gifu Prefecture and how these women evaluate different kinds of postnatal care services. The results show that after a 2-day hospital stay, women from Gero City preferred to move to the new postnatal care facility over the other alternatives (continued hospitalization or discharge home). In addition, the estimated choice probabilities for selecting the postnatal care facility under different scenarios show a high level of acceptance for this new postnatal care facility.

Effectiveness of an Imaging Plate System in Emergency 131I Thyroid Monitoring.

Abstract: The effect of temperature and shielding on the lower detection limit of a thyroid I monitoring system was investigated in an anthropomorphic thyroid-neck phantom fitted with an imaging plate. The phantom was loaded with an I aqueous solution and monitored with the imaging plate for 10 min. After exposure, the plates were incubated with or without the shield at 0, 10, 20, 25, 30, or 40°C. The latent image was read out at 0 min to 7 d after exposure. The thyroid equivalent doses corresponding to the detection limit were calculated in six age categories, using the inhalation equivalent dose coefficients recommended by the International Commission on Radiological Protection. The detection limit was distributed between 0.13 and 4.2 kBq, and depended on the age of subjects, elapsed time, temperature, and shielding provision. The maximum detection limit of 4.2 kBq was below the emergency screening level of 30 kBq in Japan. The thyroid equivalent dose corresponding to the detection limit ranged from 0.17 to 46 mSv. From the maximum equivalent dose of 46 mSv, the effective dose was estimated as 1.8 mSv, lower than the annual effective dose limit of 20 mSv for radiation workers. At 2 d after exposure, the measured dose was below the annual effective dose limit of 1 mSv for the public, regardless of age, temperature, and shielding provision. The imaging plate system effectively monitors the I thyroid levels in emergency situations.

Identification of breast tissue using the x-ray image measured with an energy-resolved cadmium telluride series detector based on photon-counting technique

Abstract: We have been developing a new mammography device with a cadmium-telluride series energy-resolved photon-counting X-ray detector. Using a photon-counting technique, we examined the sensitivity of the system for differentiating the composition of breast tissue and detecting breast tumors. To differentiate breast tissues, we prepared surgically resected specimens fixed in formalin, consisting of adipose, mammary-gland, and tumor tissues. In order to obtain the values of certain effective atomic numbers, we prepared phantoms with 0%, 50% and 100% simulated mammary-gland tissue. In our imaging system, the X-ray spectrum penetrating the object was measured using three energy bins, and the products of linear attenuation coefficients and thicknesses for the three bins were calculated. These linear attenuation coefficients were properly corrected for beam hardening and normalized, to ignore the thickness. These calculations were applied for each pixel, and the gravity point per ROI (region of interest) was plotted on scatterplots to examine their distribution. Adiposetissue values were similar to known values; however, mammary-gland values were distant from expected values. In most specimens, the tumor points were focused; however, in some specimens, it was difficult to distinguish between tumor and mammary-gland tissues given their close linear attenuation coefficients. Mammary-gland tissues may have been influenced by formalin, given its tubular structure.