Institution
Henan University of Technology
Education•Zhengzhou, China•
About: Henan University of Technology is a education organization based out in Zhengzhou, China. It is known for research contribution in the topics: Catalysis & Starch. The organization has 7648 authors who have published 6503 publications receiving 73067 citations. The organization is also known as: Hénán Gōngyè Dàxué.
Papers published on a yearly basis
Papers
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TL;DR: The results indicate that the screening protocol in the present study achieves the highest suitability and efficiency for DNA isolation from raw soya seed flour.
Abstract: Soybean is the most important genetically modified (GM) oilseed worldwide. Regulations relating to the approval of biotech soybean varieties and product labeling demand accurate and reliable detection techniques to screen for GM soya. High-quality extracted DNA is essential for DNA-based monitoring methods. Thus, four widely used protocols (SDS, CTAB, DP305, and DNeasy Plant Mini Kit) were compared in the present study to explore the most efficient DNA extraction method for raw soya matrix. The SDS-based method showed the highest applicability. Then crucial factors influencing DNA yield and purity, such as SDS lysis buffer component concentrations and organic compounds used to isolate DNA, were further investigated to improve the DNA obtained from raw soybean seeds, which accounts for the innovation of this work. As a result, lysis buffer (2% SDS (w/v), 150 mM NaCl, 50 mM Tris/HCl, 50 mM EDTA, pH 8.0) and organic reagents including chloroform/isoamyl alcohol (24:1, v/v) (C: I), isopropanol, and ethanol corresponding to the extraction and first and second precipitation procedures, respectively, were used in the optimized SDS method. The optimized method was verified by extracting approximately 2020–2444 ng DNA/mg soybean with A260/280 ratios of 1.862–1.954 from five biotech and non-biotech soybean varieties. Only 0.5 mg of soya was required to obtain enough DNA for PCR amplification using the optimized SDS-based method. These results indicate that the screening protocol in the present study achieves the highest suitability and efficiency for DNA isolation from raw soya seed flour.
41 citations
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TL;DR: In this mini-review, recent advances in EA sensor based on various strategies/nanomaterials for CAP detection and elaborate their merits as well as drawbacks were summarized to clarify future research areas for researchers in the field of EA sensor forCAP detection, and further optimize current EA sensor.
41 citations
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TL;DR: The present study demonstrates that the fabricated FITC-FA-BSA-SPIO NPs hold promising perspectives by providing a dual-modal imaging as non-toxic and target-specific vehicles in human brain tumor treatment in future.
41 citations
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TL;DR: It was determined that a moderate temperature and short time are suitable for the preparation of polysaccharides of soy hulls, which can reduce the environmental impact of waste from the food industries.
41 citations
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TL;DR: In this article, the preparation of ferrate(VI) by the anodic dissolution of an iron wire gauze in concentrated NaOH solution is described, and an anolyte of 0.35-0.48 M Na2FeO4 can be produced during 3-6 h electrolysis in initial 16 M NaO solution at 35 degrees C.
41 citations
Authors
Showing all 7708 results
Name | H-index | Papers | Citations |
---|---|---|---|
Xin Li | 114 | 2778 | 71389 |
Yang Liu | 82 | 1695 | 33657 |
Qing-Hua Qin | 52 | 505 | 9939 |
Dong-Qing Wei | 48 | 418 | 7839 |
Feng Qi | 47 | 581 | 10687 |
Jian Jian Li | 46 | 119 | 7577 |
Hongshun Yang | 46 | 165 | 5539 |
Shuangqiang Chen | 41 | 73 | 5539 |
Fei Xu | 40 | 314 | 6102 |
Dennis R. Salahub | 39 | 132 | 9259 |
Lingbo Qu | 37 | 291 | 4894 |
Yuting Wang | 37 | 80 | 11820 |
Zhiyong Jiang | 36 | 135 | 3559 |
Baoping Tang | 31 | 83 | 2455 |
Jinliang Liu | 30 | 107 | 2317 |