Henan University of Technology

About: Henan University of Technology is a education organization based out in Zhengzhou, China. It is known for research contribution in the topics: Catalysis & Starch. The organization has 7648 authors who have published 6503 publications receiving 73067 citations. The organization is also known as: Hénán Gōngyè Dàxué.

A novel impedimetric aptasensor based on AuNPs–carboxylic porous carbon for the ultrasensitive detection of ochratoxin A

Abstract: A novel aptasensor based on AuNPs–carboxylic porous carbon (cPC) is developed for the ultrasensitive detection of ochratoxin A (OTA) via electrochemical impedance spectroscopy The prepared AuNPs–cPC, which combines the porous structure of cPC with the good conductivity of AuNPs, is used as an excellent carrier for the immobilization of capture DNA (cDNA), which enhances the loading of the aptamer and amplifies the impedimetric signal When the target OTA is present, due to the complex effect of the three-dimensional macroporous structure of the AuNPs–cPC on the immobilization of cDNA, hybridization between the aptamer and cDNA and the conformational change of the OTA-aptamer, the OTA-aptamer complex may not be released from the electrode surface, which leads to an increase in the Rct signal Through optimization of the important parameters, the optimal aptamer concentration of 10 μmol L−1 and optimal incubation time of 9 min are obtained Under the optimal conditions, the ΔRct is linearly proportional to OTA concentration in a logarithmic way in the range of 1 × 10−8 to 01 ng mL−1, and the actual limit of detection is 1 × 10−8 ng mL−1

Highly Selective Turn-On Fluorescent Chemodosimeter for AlIII Detection through AlIII-Promoted Hydrolysis of C=N Double Bonds in the 8-Hydroxyquinoline Aldehyde Schiff Base

Abstract: Fluorescent chemodosimeters based on small organic molecules are widely used in the detection of various analytes. However, chemodosimeters for metal ion detection are still limited to a few transition metals, such as HgII , CuII , FeIII , AuIII , etc. In this work, a fluorescent chemodosimeter of 5-chloro-7-phenyliminomethyl-8-hydroxyquinoline (2) for the detection of AlIII is reported. Chemodosimeter 2 exhibits a turn-on fluorescence response to AlIII owing to an AlIII -promoted hydrolysis of carbon-nitrogen double bonds. This reaction yielded a 1-Al complex, which exhibited high fluorescence emission at 487 nm based on inhibition of excited-state intramolecular proton transfer (ESIPT). Because of the specific catalytic ability of AlIII , the selectivity of 2 to AlIII is excellent both in aqueous solutions and in solid matrix. The fluorescence enhancement was as high as 582-fold and the turn-on fluorescence can be observed even by the naked eye upon an irradiation with a UV lamp. The detection limit of 2 for AlIII sensing is 54 nmol L-1 and the linear range is 0-50 μmol L-1 . This work provides a new strategy for designing main-group-metal chemodosimeters based on small organic molecules.

Functional and Proteomic Investigations Reveal Major Royal Jelly Protein 1 Associated with Anti-hypertension Activity in Mouse Vascular Smooth Muscle Cells.

Abstract: Vascular smooth muscle cells (VSMCs) are a major cell type of the arterial wall and their functionality is associated with blood pressure regulation. Although royal jelly (RJ) has reported effects on anti-hypertension, the mechanism of blood pressure regulation by major royal jelly protein 1 (MRJP1), the most abundant RJ protein, is still unknown. The mrjp1 gene was inserted into mouse VSMCs to investigate how MRJP1 influences VSMC functionality by functional and proteomic analysis. The expression of MRJP1 in VSMCs significantly reduced cell contraction, migration, and proliferation, suggesting a potential role in decreasing hypertension via action on VSMCs. These anti-hypertension activities were further observed in the changes of the proteome setting of mouse VSMCs. Among 675 different proteins after MRJP1 expression, 646 were down-regulated and significantly enriched in pathways implicated in VSMC contraction and migration, which suggest MRJP1 lowers VSMC contraction and migration by inhibiting muscle filament movement. The down-regulated proteins also enriched pathways in proliferation, indicating that MRJP1 hinders VSMC proliferation by reducing the supply of energy and genetic material. This is the first report integrating MRJP1 into VSMC, revealing the function and mechanism correlated with anti-hypertensive activity. This offers a therapeutic potential to control hypertension by gene-therapy using bee-products.