Henan University of Technology

About: Henan University of Technology is a education organization based out in Zhengzhou, China. It is known for research contribution in the topics: Catalysis & Starch. The organization has 7648 authors who have published 6503 publications receiving 73067 citations. The organization is also known as: Hénán Gōngyè Dàxué.

Molecular Design, Structural Analysis and Antifungal Activity of Derivatives of Peptide CGA-N46.

Abstract: Chromogranin A (CGA)-N46, a derived peptide of human chromogranin A, has antifungal activity. To further research the active domain of CGA-N46, a series of derivatives were designed by successively deleting amino acid from both terminus of CGA-N46, and the amino acid sequence of each derivative was analyzed by bioinformatic software. Based on the predicted physicochemical properties of the peptides, including half-life time in mammalian reticulocytes (in vitro), yeast (in vivo) and E. coli (in vivo), instability index, aliphatic index and grand average of hydropathicity (GRAVY), the secondary structure, net charge, the distribution of hydrophobic residues and hydrophilic residues, the final derivatives CGA-N15, CGA-N16, CGA-N12 and CGA-N8 were synthesized by solid-phase peptide synthesis. The results of bioinformatic analysis showed that CGA-N46 and its derivatives were α-helix, neutral or weak positive charge, hydrophilic, and CGA-N12 and CGA-N8 were more stable than the other derivatives. The results of circular dichroism confirmed that CGA-N46 and its derived peptides displayed α-helical structure in an aqueous solution and 30 mM sodium dodecylsulfate, but α-helical contents decreased in hydrophobic lipid vesicles. CGA-N15, CGA-N16, CGA-N12 and CGA-N8 had higher antifungal activities than their mother peptide CGA-N46. Among of the derived peptides, CGA-N12 showed the least hemolytic activity. In conclusion, we have successfully identified the active domain of CGA-N46 with strong antifungal activity and weak hemolytic activity, which provides the possibility to develop a new class of antibiotics.

Immobilization of gold nanoparticles on multi-wall carbon nanotubes as an enhanced material for selective voltammetric determination of dopamine

Abstract: A novel and simple procedure is designed to prepare well-dispersed Au nanoparticles on multi-wall carbon nanotubes (MWNTs). The morphology and structure of the as-prepared Au/MWNTs nanocomposite were characterized by scanning electron microscopy (SEM) and energy dispersive spectrometer (EDS). Au nanoparticles effectively anchored on MWNTs. The excellent electrocatalytic activity of the Au/MWNTs nanocomposite for the oxidation of dopamine was studied by voltammetry.

Synthesis of pillar[n]arenes (n = 5 and 6) with deep eutectic solvent choline chloride 2FeCl3

Abstract: A novel and distinct method of preparing pillar[5]arene and pillar[6]arene with high selectivity and efficiency has been achieved by condensation of 1,4-dialkoxybenzene and paraformaldehyde with the choline chloride (ChCl)/ferric chloride (FeCl3) deep eutectic solvent in CH2Cl2 at room temperature. Under the optimal conditions, the yield of pillar[5]arene and pillar[6]arene is 35% and 53%, respectively. The reaction mechanism is investigated by room-temperature X-band Electron Spin Resonance (ESR), indicating that a free radical takes part in this cyclization reaction and acts as an intermediate. Our research is the first report about the application of DESs in supramolecular macrocyclic host synthesis.

A sensitive electrochemical sensor based on reduced graphene oxide/Fe3O4 nanorod composites for detection of nitrofurantoin and its metabolite

Abstract: In this work, a reduced graphene oxide/Fe3O4 nanorod composite (rGO/Fe3O4NR) modified glassy carbon electrode was fabricated for nitrofurantoin (NFT) and 1-aminohydantoin (AHD) detection. The structure of the synthesized nanocomposites was confirmed by transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction (XRD). We synthesized rGO/Fe3O4NR composites with the advantages of larger specific surface area, more active sites, and higher conductivity than those of a single material. Meanwhile, they exhibited higher catalytic activity and efficiently accelerated electron transfer, thereby greatly enhancing the electrode’s performance. Under optimal conditions, the modified electrode exhibited a linear relationship in the NFT concentration range from 0.005 to 100 μmol L−1 with a detection limit of 1.14 nmol L−1. The linear range of AHD was 0.1 to 100 μmol L−1 with a detection limit of 83.1 nmol L−1. The recoveries of NFT and AHD were 91.10–94.07% and 92.93–97.94%, respectively. In addition, the proposed electrode displayed an excellent analytical performance with great reproducibility, stability and anti-interference ability. It was successfully utilized for the determination of NFT and AHD in real samples.