scispace - formally typeset
Search or ask a question
Institution

Hokkaido University

EducationSapporo, Hokkaidô, Japan
About: Hokkaido University is a education organization based out in Sapporo, Hokkaidô, Japan. It is known for research contribution in the topics: Population & Catalysis. The organization has 53925 authors who have published 115403 publications receiving 2651647 citations. The organization is also known as: Hokudai & Hokkaidō daigaku.
Topics: Population, Catalysis, Gene, Transplantation, Virus


Papers
More filters
Journal ArticleDOI
TL;DR: The key role of E1 was demonstrated in repressing flowering and delaying maturity in soybean, suggesting its response to photoperiod and its dominant effect induced by long day length.
Abstract: The complex and coordinated regulation of flowering has high ecological and agricultural significance The maturity locus E1 has a large impact on flowering time in soybean, but the molecular basis for the E1 locus is largely unknown Through positional cloning, we delimited the E1 locus to a 174-kb region containing an intron-free gene (E1) The E1 protein contains a putative bipartite nuclear localization signal and a region distantly related to B3 domain In the recessive allele, a nonsynonymous substitution occurred in the putative nuclear localization signal, leading to the loss of localization specificity of the E1 protein and earlier flowering The early-flowering phenotype was consistently observed in three ethylmethanesulfonate-induced mutants and two natural mutations that harbored a premature stop codon or a deletion of the entire E1 gene E1 expression was significantly suppressed under short-day conditions and showed a bimodal diurnal pattern under long-day conditions, suggesting its response to photoperiod and its dominant effect induced by long day length When a functional E1 gene was transformed into the early-flowering cultivar Kariyutaka with low E1 expression, transgenic plants carrying exogenous E1 displayed late flowering Furthermore, the transcript abundance of E1 was negatively correlated with that of GmFT2a and GmFT5a, homologues of FLOWERING LOCUS T that promote flowering These findings demonstrated the key role of E1 in repressing flowering and delaying maturity in soybean The molecular identification of the maturity locus E1 will contribute to our understanding of the molecular mechanisms by which a short-day plant regulates flowering time and maturity

344 citations

Journal ArticleDOI
TL;DR: The long-term effects of different management practices on soil microbial biomass (SMB) (substrate-induced respiration (SIR) and chloroform fumigation incubation (CFI)) and micro-flora physiological and catabolic diversity (BIOLOG TM ecoplate well system) were evaluated by the International Maize and Wheat Improvement Center (CIMMYT) at its semi-arid highland experiment station in Mexico.

344 citations

Journal ArticleDOI
TL;DR: It is shown that pravastatin induced the VEGF-like angiogenic factor placental growth factor (PGF) and ameliorated the symptoms of preeclampsia, and the model identifies low-dose statins and PGF as candidates for preeClampsia treatment.
Abstract: Preeclampsia is a relatively common pregnancy-related disorder. Both maternal and fetal lives will be endangered if it proceeds unabated. Recently, the placenta-derived anti-angiogenic factors, such as soluble fms-like tyrosine kinase-1 (sFLT1) and soluble endoglin (sENG), have attracted attention in the progression of preeclampsia. Here, we established a unique experimental model to test the role of sFLT1 in preeclampsia using a lentiviral vector-mediated placenta-specific expression system. The model mice showed hypertension and proteinuria during pregnancy, and the symptoms regressed after parturition. Intrauterine growth restriction was also observed. We further showed that pravastatin induced the VEGF-like angiogenic factor placental growth factor (PGF) and ameliorated the symptoms. We conclude that our experimental preeclamptic murine model phenocopies the human case, and the model identifies low-dose statins and PGF as candidates for preeclampsia treatment.

344 citations

Journal ArticleDOI
TL;DR: Although no prospective trial has yet been completed to evaluate the clinical significance of these serum markers, this literature survey suggests that combinations of CEA, CA19-9, and CA72-4 are the most effective ways for staging before surgery or chemotherapy.
Abstract: The aim of this review was to evaluate the clinical significance of serum tumor markers, particularly CEA, CA19-9, and CA72-4, in patients with gastric cancer. A systematic literature search was performed using PubMed/MEDLINE with the keywords “gastric cancer” and “tumor marker,” to select 4,925 relevant reports published before the end of November 2012. A total of 187 publications contained data for CEA and CA19-9, and 19 publications contained data related to all three tumor markers. The positive rates were 21.1 % for CEA, 27.8 % for CA19-9, and 30.0 % for CA72-4. These three markers were significantly associated with tumor stage and patient survival. Serum markers are not useful for early cancer, but they are useful for detecting recurrence and distant metastasis, predicting patient survival, and monitoring after surgery. Tumor marker monitoring may be useful for patients after surgery because the positive conversion of tumor markers usually occurs 2–3 months before imaging abnormalities. Among other tumor markers, alpha-fetoprotein (AFP) is useful for detecting and predicting liver metastases. Moreover, CA125 and sialyl Tn antigens (STN) are useful for detecting peritoneal metastases. Although no prospective trial has yet been completed to evaluate the clinical significance of these serum markers, this literature survey suggests that combinations of CEA, CA19-9, and CA72-4 are the most effective ways for staging before surgery or chemotherapy. In particular, monitoring tumor markers that were elevated before surgery or chemotherapy could be useful for detection of recurrence or evaluation of the response.

343 citations

Journal ArticleDOI
TL;DR: Mechanistic evidence is provided that NEAT1 promotes ATR signaling in response to replication stress and is thereby engaged in a negative feedback loop that attenuates oncogene-dependent activation of p53.
Abstract: In a search for mediators of the p53 tumor suppressor pathway, which induces pleiotropic and often antagonistic cellular responses, we identified the long noncoding RNA (lncRNA) NEAT1. NEAT1 is an essential architectural component of paraspeckle nuclear bodies, whose pathophysiological relevance remains unclear. Activation of p53, pharmacologically or by oncogene-induced replication stress, stimulated the formation of paraspeckles in mouse and human cells. Silencing Neat1 expression in mice, which prevents paraspeckle formation, sensitized preneoplastic cells to DNA-damage-induced cell death and impaired skin tumorigenesis. We provide mechanistic evidence that NEAT1 promotes ATR signaling in response to replication stress and is thereby engaged in a negative feedback loop that attenuates oncogene-dependent activation of p53. NEAT1 targeting in established human cancer cell lines induced synthetic lethality with genotoxic chemotherapeutics, including PARP inhibitors, and nongenotoxic activation of p53. This study establishes a key genetic link between NEAT1 paraspeckles, p53 biology and tumorigenesis and identifies NEAT1 as a promising target to enhance sensitivity of cancer cells to both chemotherapy and p53 reactivation therapy.

343 citations


Authors

Showing all 54156 results

NameH-indexPapersCitations
Shizuo Akira2611308320561
Yi Cui2201015199725
John F. Hartwig14571466472
Yoshihiro Kawaoka13988375087
David Y. Graham138104780886
Takashi Kadowaki13787389729
Kazunari Domen13090877964
Susumu Kitagawa12580969594
Toshikazu Nakamura12173251374
Toshio Hirano12040155721
Li-Jun Wan11363952128
Wenbin Lin11347456786
Xiaoming Li113193272445
Jinhua Ye11265849496
Terence Tao11160694316
Network Information
Related Institutions (5)
Kyoto University
217.2K papers, 6.5M citations

97% related

University of Tokyo
337.5K papers, 10.1M citations

97% related

Nagoya University
128.2K papers, 3.2M citations

97% related

Tohoku University
170.7K papers, 3.9M citations

96% related

Osaka University
185.6K papers, 5.1M citations

96% related

Performance
Metrics
No. of papers from the Institution in previous years
YearPapers
2023127
2022427
20214,743
20204,805
20194,363
20184,112