Hokkaido University

About: Hokkaido University is a education organization based out in Sapporo, Hokkaidô, Japan. It is known for research contribution in the topics: Population & Catalysis. The organization has 53925 authors who have published 115403 publications receiving 2651647 citations. The organization is also known as: Hokudai & Hokkaidō daigaku.

The MHC class I ligand-generating system: roles of immunoproteasomes and the interferon-4gMY-inducible proteasome activator PA28

Abstract: Production of antigenic peptides that serve as MHC class I ligands is essential for initiation of cell-mediated immunity. Accumulating evidence indicates that the proteasome, a large multisubunit protein deg radative machine in eukaryotes, functions as a processing enzyme responsible for the generation of MHC class I ligands. This processing system is elaborately regulated by various immunomodulatory cytokines. In particular, interferon-gamma induces the formation of immunoproteasomes and a recently identified proteasomal regulatory factor. PA28, which in concert contribute to efficient production of MHC class I ligands. Many of the MHC-encoded genes including LMP appear to have emerged by an ancient chromosomal duplication, suggesting that modifications and renewal of pre-existing non-immune genes were instrumental in the emergence of adaptive immunity.

Visible light activity of rare earth metal doped (Er3+, Yb3+ or Er3+/Yb3+) titania photocatalysts

Abstract: A series of Er3+-TiO2, Yb3+-TiO2 and Er3+/Yb3+-TiO2 photocatalysts were obtained via sol–gel method, using lanthanides precursor ranging from 0.25 to 10 mol%. The experiments demonstrated that phenol in aqueous solutions was successfully degraded under visible light (λ > 450 nm) using Er/Yb-TiO2. The as-prepared samples were characterized by X-ray diffraction (XRD), X-ray photoelectron emission spectroscopy (XPS), UV–vis absorption measurement, BET surface area analysis and luminescent spectroscopy. XPS analysis revealed that erbium and ytterbium were present in the form of oxides. The sample showing the highest photoactivity was in the form of anatase, its surface area equalled to 125 m2/g, average crystals size was 13 nm, and it was prepared introducing 1 mol% of Yb3+ into reaction medium. 3 h of irradiation resulted in 89% of phenol degradation under visible light. Action spectra analysis performed for the selected Er/Yb-TiO2 samples, revealed that irradiation from 420 to 475 nm is responsible for visible light photoactivity.

A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy

Abstract: Dual-color fluorescence cross-correlation spectroscopy (FCCS) is a promising technique for quantifying protein-protein interactions1,2,3,4,5 In this technique, two different fluorescent labels are excited and detected simultaneously within a common measurement volume Difficulties in aligning two laser lines and emission crossover between the two fluorophores, however, make this technique complex To overcome these limitations, we developed a fluorescent protein with a large Stokes shift This protein, named Keima, absorbs and emits light maximally at 440 nm and 620 nm, respectively Combining a monomeric version of Keima with cyan fluorescent protein allowed dual-color FCCS with a single 458-nm laser line and complete separation of the fluorescent protein emissions This FCCS approach enabled sensitive detection of proteolysis by caspase-3 and the association of calmodulin with calmodulin-dependent enzymes In addition, Keima and a spectral variant that emits maximally at 570 nm might facilitate simultaneous multicolor imaging with single-wavelength excitation

Lake volume and groundwater storage variations in Tibetan Plateau's endorheic basin

Abstract: The Tibetan Plateau (TP), the highest and largest plateau in the world, with complex and competing cryospheric‐hydrologic‐geodynamic processes, is particularly sensitive to anthropogenic warming. The quantitative water mass budget in the TP is poorly known. Here we examine annual changes in lake area, level, and volume during 1970s–2015. We find that a complex pattern of lake volume changes during 1970s–2015: a slight decrease of −2.78 Gt yr−1 during 1970s–1995, followed by a rapid increase of 12.53 Gt yr−1 during 1996–2010, and then a recent deceleration (1.46 Gt yr−1) during 2011–2015. We then estimated the recent water mass budget for the Inner TP, 2003–2009, including changes in terrestrial water storage, lake volume, glacier mass, snow water equivalent (SWE), soil moisture, and permafrost. The dominant components of water mass budget, namely, changes in lake volume (7.72 ± 0.63 Gt yr−1) and groundwater storage (5.01 ± 1.59 Gt yr−1), increased at similar rates. We find that increased net precipitation contributes the majority of water supply (74%) for the lake volume increase, followed by glacier mass loss (13%), and ground ice melt due to permafrost degradation (12%). Other term such as SWE (1%) makes a relatively small contribution. These results suggest that the hydrologic cycle in the TP has intensified remarkably during recent decades.